Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Clostridium lundense sp nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen

Cirne, Dores LU ; Delgado, Osvaldo LU ; Marichamy, Sankar LU and Mattiasson, Bo LU (2006) In International Journal of Systematic and Evolutionary Microbiology 56(3). p.625-628
Abstract
A strictly anaerobic, mesophilic, endospore-forming, lipolytic bacterium, designated strain R1(T), was isolated from bovine rumen fluid and characterized. Cells of this isolate were Gram-positive, non-motile rods that formed spherical terminal spores. The overall biochemical and physiological characteristics indicated that this strain should be placed in the genus Clostridium. The strain grew at temperatures between 25 and 47 degrees C (optimum, 37 degrees C), at pH between 5(.)0 and 8(.)5 (optimum pH 5(.)5-7(.)0) and in NaCl concentrations of 0-3% (w/v). The isolate was not able to utilize glucose or other carbohydrates as carbon sources. The DNA G+C content was 31(.)2 mol%. Sequence analysis of the 16S rRNA gene of R1(T) revealed that it... (More)
A strictly anaerobic, mesophilic, endospore-forming, lipolytic bacterium, designated strain R1(T), was isolated from bovine rumen fluid and characterized. Cells of this isolate were Gram-positive, non-motile rods that formed spherical terminal spores. The overall biochemical and physiological characteristics indicated that this strain should be placed in the genus Clostridium. The strain grew at temperatures between 25 and 47 degrees C (optimum, 37 degrees C), at pH between 5(.)0 and 8(.)5 (optimum pH 5(.)5-7(.)0) and in NaCl concentrations of 0-3% (w/v). The isolate was not able to utilize glucose or other carbohydrates as carbon sources. The DNA G+C content was 31(.)2 mol%. Sequence analysis of the 16S rRNA gene of R1(T) revealed that it has the closest match (98 % similarity) with Clostridium tetanomorphum DSM 4474(T). The highest levels of DNA-DNA relatedness of the isolate were 61(.)9 and 54(.)3 % with Clostridium pascui DSM 10365(T) and C. tetanomorphum DSM 4474(T), respectively. Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA-DNA hybridization data and distinct phenotypic characteristics, strain R1(T) (=DSM 17049(T)=CCUG 50446(T)) was classified in the genus Clostridium, as a member of a novel species, for which the name Clostridium lundense sp. nov. is proposed. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Systematic and Evolutionary Microbiology
volume
56
issue
3
pages
625 - 628
publisher
Microbiology Society
external identifiers
  • wos:000236519800022
  • pmid:16514039
  • scopus:33645225420
ISSN
1466-5026
DOI
10.1099/ijs.0.63730-0
language
English
LU publication?
yes
id
4a4f6698-fbac-4db9-9839-dba197033a63 (old id 414449)
date added to LUP
2016-04-01 12:29:13
date last changed
2021-03-03 02:33:37
@article{4a4f6698-fbac-4db9-9839-dba197033a63,
  abstract     = {A strictly anaerobic, mesophilic, endospore-forming, lipolytic bacterium, designated strain R1(T), was isolated from bovine rumen fluid and characterized. Cells of this isolate were Gram-positive, non-motile rods that formed spherical terminal spores. The overall biochemical and physiological characteristics indicated that this strain should be placed in the genus Clostridium. The strain grew at temperatures between 25 and 47 degrees C (optimum, 37 degrees C), at pH between 5(.)0 and 8(.)5 (optimum pH 5(.)5-7(.)0) and in NaCl concentrations of 0-3% (w/v). The isolate was not able to utilize glucose or other carbohydrates as carbon sources. The DNA G+C content was 31(.)2 mol%. Sequence analysis of the 16S rRNA gene of R1(T) revealed that it has the closest match (98 % similarity) with Clostridium tetanomorphum DSM 4474(T). The highest levels of DNA-DNA relatedness of the isolate were 61(.)9 and 54(.)3 % with Clostridium pascui DSM 10365(T) and C. tetanomorphum DSM 4474(T), respectively. Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA-DNA hybridization data and distinct phenotypic characteristics, strain R1(T) (=DSM 17049(T)=CCUG 50446(T)) was classified in the genus Clostridium, as a member of a novel species, for which the name Clostridium lundense sp. nov. is proposed.},
  author       = {Cirne, Dores and Delgado, Osvaldo and Marichamy, Sankar and Mattiasson, Bo},
  issn         = {1466-5026},
  language     = {eng},
  number       = {3},
  pages        = {625--628},
  publisher    = {Microbiology Society},
  series       = {International Journal of Systematic and Evolutionary Microbiology},
  title        = {Clostridium lundense sp nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen},
  url          = {http://dx.doi.org/10.1099/ijs.0.63730-0},
  doi          = {10.1099/ijs.0.63730-0},
  volume       = {56},
  year         = {2006},
}