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Determining Short Chain Fatty Acids in Sewage Sludge Hydrolysate: A Comparison of Three Analytical Methods and Investigation of Sample Storage Effects

Ibrahim, Victor LU ; Hey, Tobias LU and Jönsson, Karin LU (2014) In Journal of Environmental Sciences(China) 26(4). p.926-933
Abstract
In anaerobic digestion (AD), the production of short-chain fatty acids (SCFAs) can be beneficial or harmful to the overall process, depending on the concentration of accumulated acids. Therefore, the accurate determination of the SCFA concentration in both fresh and stored sludge hydrolysates is important. To select a suitable method for monitoring SCFAs during the AD of sewage sludge, the accuracy of three available analytical methods, including 5 pH point acid titration (TITRA5), gas chromatography (GC), and spectrophotometry, were compared in the present study. The results revealed that TITRA5 and GC displayed better agreement in the achieved measurements and higher precision and accuracy than the spectrophotometric assay, as supported... (More)
In anaerobic digestion (AD), the production of short-chain fatty acids (SCFAs) can be beneficial or harmful to the overall process, depending on the concentration of accumulated acids. Therefore, the accurate determination of the SCFA concentration in both fresh and stored sludge hydrolysates is important. To select a suitable method for monitoring SCFAs during the AD of sewage sludge, the accuracy of three available analytical methods, including 5 pH point acid titration (TITRA5), gas chromatography (GC), and spectrophotometry, were compared in the present study. The results revealed that TITRA5 and GC displayed better agreement in the achieved measurements and higher precision and accuracy than the spectrophotometric assay, as supported by the application of different statistical models. TITRA5 excelled in titrating unfiltered hydrolysate while simultaneously measuring the alkalinity, whereas the GC method provided detailed information on the contribution of different fatty acids to the total acidity. In contrast, the spectrophotometric assay suffered from many forms of interference, depending on the sample’s matrix. SCFA production followed the pattern of enzymatic reactions and fitted the Michaelis-Menten model. In addition to promoting TITRA5 as an accurate and robust analytical tool for routine SCFA analyses, this comparative study also demonstrated the possibility of storing hydrolysate samples at different temperatures and durations without altering the SCFA measurements. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Five pH point titration, gas chromatography, spectrophotometry, short-chain fatty acids, sludge hydrolysis, sample storage
in
Journal of Environmental Sciences(China)
volume
26
issue
4
pages
926 - 933
publisher
Science China Press
external identifiers
  • wos:000334259000024
  • pmid:25079424
  • scopus:84898618410
ISSN
1001-0742
DOI
10.1016/S1001-0742(13)60516-1
language
English
LU publication?
yes
id
e5d0dd04-87a1-49b2-aef8-1fc9a29022da (old id 4145358)
date added to LUP
2013-11-12 16:27:00
date last changed
2017-08-27 03:24:55
@article{e5d0dd04-87a1-49b2-aef8-1fc9a29022da,
  abstract     = {In anaerobic digestion (AD), the production of short-chain fatty acids (SCFAs) can be beneficial or harmful to the overall process, depending on the concentration of accumulated acids. Therefore, the accurate determination of the SCFA concentration in both fresh and stored sludge hydrolysates is important. To select a suitable method for monitoring SCFAs during the AD of sewage sludge, the accuracy of three available analytical methods, including 5 pH point acid titration (TITRA5), gas chromatography (GC), and spectrophotometry, were compared in the present study. The results revealed that TITRA5 and GC displayed better agreement in the achieved measurements and higher precision and accuracy than the spectrophotometric assay, as supported by the application of different statistical models. TITRA5 excelled in titrating unfiltered hydrolysate while simultaneously measuring the alkalinity, whereas the GC method provided detailed information on the contribution of different fatty acids to the total acidity. In contrast, the spectrophotometric assay suffered from many forms of interference, depending on the sample’s matrix. SCFA production followed the pattern of enzymatic reactions and fitted the Michaelis-Menten model. In addition to promoting TITRA5 as an accurate and robust analytical tool for routine SCFA analyses, this comparative study also demonstrated the possibility of storing hydrolysate samples at different temperatures and durations without altering the SCFA measurements.},
  author       = {Ibrahim, Victor and Hey, Tobias and Jönsson, Karin},
  issn         = {1001-0742},
  keyword      = {Five pH point titration,gas chromatography,spectrophotometry,short-chain fatty acids,sludge hydrolysis,sample storage},
  language     = {eng},
  number       = {4},
  pages        = {926--933},
  publisher    = {Science China Press},
  series       = {Journal of Environmental Sciences(China)},
  title        = {Determining Short Chain Fatty Acids in Sewage Sludge Hydrolysate: A Comparison of Three Analytical Methods and Investigation of Sample Storage Effects},
  url          = {http://dx.doi.org/10.1016/S1001-0742(13)60516-1},
  volume       = {26},
  year         = {2014},
}