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Monitoring monohydroperoxides in docosahexaenoic acid using high-performance liquid chromatography

Lyberg, Ann-Marie LU and Adlercreutz, Patrick LU orcid (2006) In Lipids 41(1). p.67-76
Abstract
The oxidation of free DHA has been investigated with respect to monohydroperoxides and polyhydroperoxides, which were analyzed with a novel HPLC method. The temperature and physical system, i.e., bulk and liposome, were varied. We have also studied the effects of antioxidants Such as alpha-tocopherol, ascorbic acid, and juice from sea buckthorn on DHA. The HPLC method, which was performed isocratically, eluted eight peaks, each containing one or two isomers of monohydroperoxy-DHA. This method showed that the double bond farthest. from the carboxyl group was easily oxidized, as shown by the rapid increase in the amount of C20-monohydroperoxy-DHA, which always provided the largest contribution to the total amount of monohydroperoxides. The... (More)
The oxidation of free DHA has been investigated with respect to monohydroperoxides and polyhydroperoxides, which were analyzed with a novel HPLC method. The temperature and physical system, i.e., bulk and liposome, were varied. We have also studied the effects of antioxidants Such as alpha-tocopherol, ascorbic acid, and juice from sea buckthorn on DHA. The HPLC method, which was performed isocratically, eluted eight peaks, each containing one or two isomers of monohydroperoxy-DHA. This method showed that the double bond farthest. from the carboxyl group was easily oxidized, as shown by the rapid increase in the amount of C20-monohydroperoxy-DHA, which always provided the largest contribution to the total amount of monohydroperoxides. The monohydroperoxy-DHA containing the hydroperoxy group located oil the double bond nearest the carboxyl group also was shown to increase considerably during an increase in the total amount of monohydroperoxides. This demonstrates that the double bonds located nearest and farthest from the carboxyl group were the most prone to hydroperoxide formation. DHA was more stable when stored in liposomes than as bulk. Addition of alpha-tocopherol to the DHA-containing liposomes reduced the oxidation of these double bonds. The antioxidant effect of alpha-tocopherol was prolonged when combined with ascorbic acid, since alpha-tocopherol was regenerated. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lipids
volume
41
issue
1
pages
67 - 76
publisher
Springer
external identifiers
  • wos:000235634800008
  • pmid:16555474
  • scopus:33644584057
ISSN
0024-4201
DOI
10.1007/s11745-006-5072-z
language
English
LU publication?
yes
id
e62008a7-6cae-42ba-b878-6a6ef4317684 (old id 417075)
date added to LUP
2016-04-01 11:34:09
date last changed
2021-02-17 03:42:08
@article{e62008a7-6cae-42ba-b878-6a6ef4317684,
  abstract     = {The oxidation of free DHA has been investigated with respect to monohydroperoxides and polyhydroperoxides, which were analyzed with a novel HPLC method. The temperature and physical system, i.e., bulk and liposome, were varied. We have also studied the effects of antioxidants Such as alpha-tocopherol, ascorbic acid, and juice from sea buckthorn on DHA. The HPLC method, which was performed isocratically, eluted eight peaks, each containing one or two isomers of monohydroperoxy-DHA. This method showed that the double bond farthest. from the carboxyl group was easily oxidized, as shown by the rapid increase in the amount of C20-monohydroperoxy-DHA, which always provided the largest contribution to the total amount of monohydroperoxides. The monohydroperoxy-DHA containing the hydroperoxy group located oil the double bond nearest the carboxyl group also was shown to increase considerably during an increase in the total amount of monohydroperoxides. This demonstrates that the double bonds located nearest and farthest from the carboxyl group were the most prone to hydroperoxide formation. DHA was more stable when stored in liposomes than as bulk. Addition of alpha-tocopherol to the DHA-containing liposomes reduced the oxidation of these double bonds. The antioxidant effect of alpha-tocopherol was prolonged when combined with ascorbic acid, since alpha-tocopherol was regenerated.},
  author       = {Lyberg, Ann-Marie and Adlercreutz, Patrick},
  issn         = {0024-4201},
  language     = {eng},
  number       = {1},
  pages        = {67--76},
  publisher    = {Springer},
  series       = {Lipids},
  title        = {Monitoring monohydroperoxides in docosahexaenoic acid using high-performance liquid chromatography},
  url          = {http://dx.doi.org/10.1007/s11745-006-5072-z},
  doi          = {10.1007/s11745-006-5072-z},
  volume       = {41},
  year         = {2006},
}