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The expression of a Pichia stipitis xylose reductase mutant with higher K-M for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae

Jeppsson, Marie LU ; Bengtsson, Oskar LU ; Franke, K ; Lee, H ; Hahn-Hägerdal, Bärbel LU and Gorwa-Grauslund, Marie-Francoise LU (2006) In Biotechnology and Bioengineering 93(4). p.665-673
Abstract
Xylose fermentation by Saccharomyces cerevisiae requires the introduction of a xylose pathway, either similar to that found in the natural xylose-utilizing yeasts Pichia stipitis and Candida shehatae or similar to the bacterial pathway. The use of NAD(P)H-dependent XR and NAD(+)-dependent XDH from P. stipitis creates a cofactor imbalance resulting in xylitol formation. The effect of replacing the native P. stipitis XR with a mutated XR with increased K-M for NADPH (Kostrzynska et al., 1998: FEMS Microbiol Lett 159:107-112) was investigated for xylose fermentation to ethanol by recombinant S. cerevisiae strains. Enhanced ethanol yields accompanied by decreased xylitol yields were obtained in strains carrying the mutated XR. Flux analysis... (More)
Xylose fermentation by Saccharomyces cerevisiae requires the introduction of a xylose pathway, either similar to that found in the natural xylose-utilizing yeasts Pichia stipitis and Candida shehatae or similar to the bacterial pathway. The use of NAD(P)H-dependent XR and NAD(+)-dependent XDH from P. stipitis creates a cofactor imbalance resulting in xylitol formation. The effect of replacing the native P. stipitis XR with a mutated XR with increased K-M for NADPH (Kostrzynska et al., 1998: FEMS Microbiol Lett 159:107-112) was investigated for xylose fermentation to ethanol by recombinant S. cerevisiae strains. Enhanced ethanol yields accompanied by decreased xylitol yields were obtained in strains carrying the mutated XR. Flux analysis showed that strains harboring the mutated XR utilized a larger fraction of NADH for xylose reduction. The overproduction of the mutated XR resulted in an ethanol yield of 0.40 g per gram of sugar and a xylose consumption rate of 0.16 g per gram of biomass per hour in chemostat culture (0.06/h) with 10 g/L glucose and 10 g/L xylose as carbon source. (c) 2005 Wiley Periodicals, Inc. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
xylitol, site-specific mutagenesis, xylose reductase, Saccharomyces cerevisiae, NAD(P)H
in
Biotechnology and Bioengineering
volume
93
issue
4
pages
665 - 673
publisher
John Wiley and Sons
external identifiers
  • wos:000235630600007
  • pmid:16372361
  • scopus:33644879465
  • pmid:16372361
ISSN
1097-0290
DOI
10.1002/bit.20737
language
English
LU publication?
yes
id
ba39a84a-d15e-4841-847e-eba0c9c69d08 (old id 417236)
date added to LUP
2016-04-01 12:26:06
date last changed
2021-04-06 01:35:45
@article{ba39a84a-d15e-4841-847e-eba0c9c69d08,
  abstract     = {Xylose fermentation by Saccharomyces cerevisiae requires the introduction of a xylose pathway, either similar to that found in the natural xylose-utilizing yeasts Pichia stipitis and Candida shehatae or similar to the bacterial pathway. The use of NAD(P)H-dependent XR and NAD(+)-dependent XDH from P. stipitis creates a cofactor imbalance resulting in xylitol formation. The effect of replacing the native P. stipitis XR with a mutated XR with increased K-M for NADPH (Kostrzynska et al., 1998: FEMS Microbiol Lett 159:107-112) was investigated for xylose fermentation to ethanol by recombinant S. cerevisiae strains. Enhanced ethanol yields accompanied by decreased xylitol yields were obtained in strains carrying the mutated XR. Flux analysis showed that strains harboring the mutated XR utilized a larger fraction of NADH for xylose reduction. The overproduction of the mutated XR resulted in an ethanol yield of 0.40 g per gram of sugar and a xylose consumption rate of 0.16 g per gram of biomass per hour in chemostat culture (0.06/h) with 10 g/L glucose and 10 g/L xylose as carbon source. (c) 2005 Wiley Periodicals, Inc.},
  author       = {Jeppsson, Marie and Bengtsson, Oskar and Franke, K and Lee, H and Hahn-Hägerdal, Bärbel and Gorwa-Grauslund, Marie-Francoise},
  issn         = {1097-0290},
  language     = {eng},
  number       = {4},
  pages        = {665--673},
  publisher    = {John Wiley and Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {The expression of a Pichia stipitis xylose reductase mutant with higher K-M for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae},
  url          = {http://dx.doi.org/10.1002/bit.20737},
  doi          = {10.1002/bit.20737},
  volume       = {93},
  year         = {2006},
}