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Micro-immuno supported liquid membrane assay (mu-ISLMA)

Tudorache, Madalina LU and Emnéus, Jenny LU (2006) In Biosensors & Bioelectronics 21(8). p.1513-1520
Abstract
A chemiluminescent (CL) based micro-immuno supported liquid membrane assay (mu-ISLMA) has been developed that enables clean up, enrichment and detection of simazine in a single miniaturised cartridge system. The mu-ISLM cartridge contains a supported liquid membrane (SLM) sandwiched between a donor and an acceptor plate (channel volumes 1.65 mu L), the latter being covered by a thin layer of gold on to which anti-simazine antibodies were covalently immobilised via a self assembled monolayer (SAM) of either dithiobis(11-aminoundecane, hydrochloride) (DTAU) or beta-mercaptoethylamine (beta-MEA). The mu-ISLMA based on DTAU was characterised by both a high apparent extraction efficiency (E-app = 136%) and high apparent enrichment factor... (More)
A chemiluminescent (CL) based micro-immuno supported liquid membrane assay (mu-ISLMA) has been developed that enables clean up, enrichment and detection of simazine in a single miniaturised cartridge system. The mu-ISLM cartridge contains a supported liquid membrane (SLM) sandwiched between a donor and an acceptor plate (channel volumes 1.65 mu L), the latter being covered by a thin layer of gold on to which anti-simazine antibodies were covalently immobilised via a self assembled monolayer (SAM) of either dithiobis(11-aminoundecane, hydrochloride) (DTAU) or beta-mercaptoethylamine (beta-MEA). The mu-ISLMA based on DTAU was characterised by both a high apparent extraction efficiency (E-app = 136%) and high apparent enrichment factor (E-e(app) = 544), which resulted in a very high sensitivity for simazine (LOD = 0.1 ng L-1). The paper discusses the influence of the different SAMs and three different anti-simazine-antibody preparations (polyclonal, affinity purified polyclonal and monoclonal) on the extraction parameters and assay sensitivity. The influence of the sample matrix (e.g. mineral water, orange juice and milk) on the simazine mu-ISLMA was also investigated. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
simazine, chemiluminescence, micro-immuno supported liquid membrane assay (mu-ISLMA), micro-immunoassay (mu-IA), self-assembled, monolayer, gold
in
Biosensors & Bioelectronics
volume
21
issue
8
pages
1513 - 1520
publisher
Elsevier
external identifiers
  • pmid:16102960
  • wos:000235492900014
  • scopus:31044445791
  • pmid:16102960
ISSN
1873-4235
DOI
10.1016/j.bios.2005.07.002
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
8e1b9458-6057-4a57-9037-b2678e58c4d5 (old id 417243)
date added to LUP
2016-04-01 15:54:56
date last changed
2021-02-18 01:04:13
@article{8e1b9458-6057-4a57-9037-b2678e58c4d5,
  abstract     = {A chemiluminescent (CL) based micro-immuno supported liquid membrane assay (mu-ISLMA) has been developed that enables clean up, enrichment and detection of simazine in a single miniaturised cartridge system. The mu-ISLM cartridge contains a supported liquid membrane (SLM) sandwiched between a donor and an acceptor plate (channel volumes 1.65 mu L), the latter being covered by a thin layer of gold on to which anti-simazine antibodies were covalently immobilised via a self assembled monolayer (SAM) of either dithiobis(11-aminoundecane, hydrochloride) (DTAU) or beta-mercaptoethylamine (beta-MEA). The mu-ISLMA based on DTAU was characterised by both a high apparent extraction efficiency (E-app = 136%) and high apparent enrichment factor (E-e(app) = 544), which resulted in a very high sensitivity for simazine (LOD = 0.1 ng L-1). The paper discusses the influence of the different SAMs and three different anti-simazine-antibody preparations (polyclonal, affinity purified polyclonal and monoclonal) on the extraction parameters and assay sensitivity. The influence of the sample matrix (e.g. mineral water, orange juice and milk) on the simazine mu-ISLMA was also investigated. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Tudorache, Madalina and Emnéus, Jenny},
  issn         = {1873-4235},
  language     = {eng},
  number       = {8},
  pages        = {1513--1520},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Micro-immuno supported liquid membrane assay (mu-ISLMA)},
  url          = {http://dx.doi.org/10.1016/j.bios.2005.07.002},
  doi          = {10.1016/j.bios.2005.07.002},
  volume       = {21},
  year         = {2006},
}