Purification and functional characterization of factor I.
Nilsson, Sara LU and Blom, Anna LU
(2014)
In Methods in Molecular Biology
1100.
p.177-188
- Abstract
- Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4179570
- author
- Nilsson, Sara
LU
and Blom, Anna
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 1100
- pages
- 177 - 188
- publisher
- Springer
- external identifiers
-
- pmid:24218260
- scopus:84934443229
- pmid:24218260
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-62703-724-2_15
- language
- English
- LU publication?
- yes
- id
- 9ab440a8-1504-4b29-953f-87e66a7526a6 (old id 4179570)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24218260?dopt=Abstract
- date added to LUP
- 2016-04-04 08:56:19
- date last changed
- 2022-01-29 07:55:47
@article{9ab440a8-1504-4b29-953f-87e66a7526a6,
abstract = {{Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.}},
author = {{Nilsson, Sara and Blom, Anna}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{177--188}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Purification and functional characterization of factor I.}},
url = {{http://dx.doi.org/10.1007/978-1-62703-724-2_15}},
doi = {{10.1007/978-1-62703-724-2_15}},
volume = {{1100}},
year = {{2014}},
}