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Purification and functional characterization of factor I.

Nilsson, Sara LU and Blom, Anna LU orcid (2014) In Methods in Molecular Biology 1100. p.177-188
Abstract
Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1100
pages
177 - 188
publisher
Springer
external identifiers
  • pmid:24218260
  • scopus:84934443229
  • pmid:24218260
ISSN
1940-6029
DOI
10.1007/978-1-62703-724-2_15
language
English
LU publication?
yes
id
9ab440a8-1504-4b29-953f-87e66a7526a6 (old id 4179570)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24218260?dopt=Abstract
date added to LUP
2016-04-04 08:56:19
date last changed
2021-08-11 01:09:50
@article{9ab440a8-1504-4b29-953f-87e66a7526a6,
  abstract     = {Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentration of approximately 35 mg/L. FI inhibits all complement pathways as it degrades activated C4b and C3b when these are bound to a cofactor such as C4b-binding protein or factor H. Here, we describe a method for purification of FI from human plasma, which is based on affinity chromatography followed by anion exchange chromatography. We also describe a functional assay, in which activity of FI can be assessed.},
  author       = {Nilsson, Sara and Blom, Anna},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {177--188},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Purification and functional characterization of factor I.},
  url          = {http://dx.doi.org/10.1007/978-1-62703-724-2_15},
  doi          = {10.1007/978-1-62703-724-2_15},
  volume       = {1100},
  year         = {2014},
}