Purification and Functional Characterization of C4b-Binding Protein (C4BP).
Mohlin, Frida LU and Blom, Anna LU
(2014)
In Methods in Molecular Biology
1100.
p.169-176
- Abstract
- C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4179575
- author
- Mohlin, Frida
LU
and Blom, Anna
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 1100
- pages
- 169 - 176
- publisher
- Springer
- external identifiers
-
- pmid:24218259
- scopus:84934444808
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-62703-724-2_14
- language
- English
- LU publication?
- yes
- id
- 77f2adb9-cf26-44e9-af99-3fd257b09075 (old id 4179575)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24218259?dopt=Abstract
- date added to LUP
- 2016-04-04 08:18:41
- date last changed
- 2022-05-16 21:13:33
@article{77f2adb9-cf26-44e9-af99-3fd257b09075,
abstract = {{C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.}},
author = {{Mohlin, Frida and Blom, Anna}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{169--176}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Purification and Functional Characterization of C4b-Binding Protein (C4BP).}},
url = {{http://dx.doi.org/10.1007/978-1-62703-724-2_14}},
doi = {{10.1007/978-1-62703-724-2_14}},
volume = {{1100}},
year = {{2014}},
}