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Purification and Functional Characterization of C4b-Binding Protein (C4BP).

Mohlin, Frida LU and Blom, Anna LU (2014) In Methods in Molecular Biology 1100. p.169-176
Abstract
C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1100
pages
169 - 176
publisher
Springer
external identifiers
  • pmid:24218259
  • scopus:84934444808
ISSN
1940-6029
DOI
10.1007/978-1-62703-724-2_14
language
English
LU publication?
yes
id
77f2adb9-cf26-44e9-af99-3fd257b09075 (old id 4179575)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24218259?dopt=Abstract
date added to LUP
2013-12-06 15:21:21
date last changed
2017-07-23 04:53:27
@article{77f2adb9-cf26-44e9-af99-3fd257b09075,
  abstract     = {C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.},
  author       = {Mohlin, Frida and Blom, Anna},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {169--176},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Purification and Functional Characterization of C4b-Binding Protein (C4BP).},
  url          = {http://dx.doi.org/10.1007/978-1-62703-724-2_14},
  volume       = {1100},
  year         = {2014},
}