Advanced

Genes for cytochrome c and cytochrome c oxidase synthesis in Bacillus subtilis

Bengtsson, Jenny LU (2001)
Abstract (Swedish)
Popular Abstract in Swedish

Cytokromer är hem-innehållande proteiner som transporterar elektroner. Hem är den järninnehållande molekyl som binder syre i hemoglobin i de röda blodkropparna i blodet hos t ex människor. Cytokromer har viktiga funktioner i olika typer av organismer: bakterier, växter och djur. Genom att studera vilka gener som behövs för syntesen av cytokromer i bakterien Bacillus subtilis kan vi lära oss om hur syntesen av cytokromer och andra metallinnehållande proteiner fungerar i andra organismer samt lära oss mer om hur bakterien Bacillus subtilis fungerar.
Abstract
Cytochromes are heme-containing proteins that function in electron transfer reactions, e.g. respiration and photosynthesis. There are several different types of cytochromes. C-type cytochromes differ from other types of cytochromes in that the heme group is covalently attached to the polypeptide via thioether bonds to two cysteinyl residues in a consensus motif, Cys-Xaa-Yaa-Cys-His. Cytochrome c oxidase is an energy transducing membrane-bound enzyme with two heme groups and two copper centres. This enzyme reduces molecular oxygen to water and is essential for animals, plants and many micro-organisms. This thesis addresses questions about how cytochrome c and cytochrome c oxidases are synthesised in living cells. From these studies one also... (More)
Cytochromes are heme-containing proteins that function in electron transfer reactions, e.g. respiration and photosynthesis. There are several different types of cytochromes. C-type cytochromes differ from other types of cytochromes in that the heme group is covalently attached to the polypeptide via thioether bonds to two cysteinyl residues in a consensus motif, Cys-Xaa-Yaa-Cys-His. Cytochrome c oxidase is an energy transducing membrane-bound enzyme with two heme groups and two copper centres. This enzyme reduces molecular oxygen to water and is essential for animals, plants and many micro-organisms. This thesis addresses questions about how cytochrome c and cytochrome c oxidases are synthesised in living cells. From these studies one also obtains general knowledge about the biosynthesis of other types of metal-containing proteins. The Gram-positive soil bacterium Bacillus subtilis was previously known to contain three c-type cytochromes: QcrC, the cytochrome c of the bc complex; CtaC, the subunit II of cytochrome c oxidase; and CccA, the cytochrome c550. In the thesis it is demonstrated that the cccB gene encodes a fourth cytochrome c, cytochrome c551. Furthermore it is shown that CtaC is a lipoprotein and that the processing of the polypeptide is important for the function of cytochrome c oxidase. By screening different libraries of mutants for deficiency in functional cytochrome c oxidase and lack of all cytochrome c the proteins encoded by the resB and resC genes were found to be important for cytochrome c synthesis in B. subtilis. Two genes, ctaG and ypmQ, were found to encode proteins important for synthesis of functional cytochrome c oxidase. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Prof Gennis, Robert B., Dept. of Biochemistry, School of Chemical Sciences, University of Illinois, USA
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Microbiology, cytochrome, cytochrome c oxidase, Bacillus subtilis, cytochrome c synthesis, bacteriology, virology, mycology, Mikrobiologi, bakteriologi, virologi, mykologi
pages
136 pages
publisher
Department of Microbiology, Lund University
defense location
Rune Grubb hall, BMC, Sölveg 21, Lund
defense date
2001-10-28 10:00
external identifiers
  • Other:ISRN: LUNBDS/NBNB-1040/1-75 (2001)
ISBN
91-628-4902-6
language
English
LU publication?
yes
id
d02421d7-bda6-4f40-bbe4-bbee9332f709 (old id 41866)
date added to LUP
2007-06-20 11:51:57
date last changed
2016-09-19 08:45:03
@phdthesis{d02421d7-bda6-4f40-bbe4-bbee9332f709,
  abstract     = {Cytochromes are heme-containing proteins that function in electron transfer reactions, e.g. respiration and photosynthesis. There are several different types of cytochromes. C-type cytochromes differ from other types of cytochromes in that the heme group is covalently attached to the polypeptide via thioether bonds to two cysteinyl residues in a consensus motif, Cys-Xaa-Yaa-Cys-His. Cytochrome c oxidase is an energy transducing membrane-bound enzyme with two heme groups and two copper centres. This enzyme reduces molecular oxygen to water and is essential for animals, plants and many micro-organisms. This thesis addresses questions about how cytochrome c and cytochrome c oxidases are synthesised in living cells. From these studies one also obtains general knowledge about the biosynthesis of other types of metal-containing proteins. The Gram-positive soil bacterium Bacillus subtilis was previously known to contain three c-type cytochromes: QcrC, the cytochrome c of the bc complex; CtaC, the subunit II of cytochrome c oxidase; and CccA, the cytochrome c550. In the thesis it is demonstrated that the cccB gene encodes a fourth cytochrome c, cytochrome c551. Furthermore it is shown that CtaC is a lipoprotein and that the processing of the polypeptide is important for the function of cytochrome c oxidase. By screening different libraries of mutants for deficiency in functional cytochrome c oxidase and lack of all cytochrome c the proteins encoded by the resB and resC genes were found to be important for cytochrome c synthesis in B. subtilis. Two genes, ctaG and ypmQ, were found to encode proteins important for synthesis of functional cytochrome c oxidase.},
  author       = {Bengtsson, Jenny},
  isbn         = {91-628-4902-6},
  keyword      = {Microbiology,cytochrome,cytochrome c oxidase,Bacillus subtilis,cytochrome c synthesis,bacteriology,virology,mycology,Mikrobiologi,bakteriologi,virologi,mykologi},
  language     = {eng},
  pages        = {136},
  publisher    = {Department of Microbiology, Lund University},
  school       = {Lund University},
  title        = {Genes for cytochrome c and cytochrome c oxidase synthesis in Bacillus subtilis},
  year         = {2001},
}