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Basophil allergen threshold sensitivity: a useful approach to anti-IgE treatment efficacy evaluation

Nopp, A ; Johansson, SGO ; Ankerst, Jaro LU orcid ; Bylin, G ; Cardell, Lars-Olaf LU ; Gronneberg, R ; Irander, K ; Palmqvist, M and Oman, H (2006) In Allergy 61(3). p.298-302
Abstract
Background: Monitoring of the allergen sensitivity of a patient is most important for optimal patient care and a basic prerequisite for immunomodulating treatment. The objective of this study was to investigate how basophil allergen sensitivity can be applied in the monitoring of anti-immunoglobulin E (IgE) treatment. Methods: Basophils from timothy grass pollen allergic patients were, by flow cytometry, analysed for allergen threshold sensitivity (CD-sens) by measuring CD63 up-regulation on CD203c-identified basophils. The results were compared with maximal percentage CD63 up-regulation at one allergen dose (CD-max), skin prick test end-point allergen titration, (SPT-sens), nasal provocation titration tests (nasal provocation titre) and... (More)
Background: Monitoring of the allergen sensitivity of a patient is most important for optimal patient care and a basic prerequisite for immunomodulating treatment. The objective of this study was to investigate how basophil allergen sensitivity can be applied in the monitoring of anti-immunoglobulin E (IgE) treatment. Methods: Basophils from timothy grass pollen allergic patients were, by flow cytometry, analysed for allergen threshold sensitivity (CD-sens) by measuring CD63 up-regulation on CD203c-identified basophils. The results were compared with maximal percentage CD63 up-regulation at one allergen dose (CD-max), skin prick test end-point allergen titration, (SPT-sens), nasal provocation titration tests (nasal provocation titre) and serum IgE and IgE antibody concentrations. Results: There was a significant correlation (r = 0.50, P = 0.01) between CD-sens and SPT-sens, CD-sens and the IgE antibody concentration in percentage of 'total IgE' (relative IgE antibody concentration) (r = 0.72, P < 0.001) as well as between CD-sens and nasal provocation titre (r = 0.54, P < 0.05) but, in contrast, CD-max did not correlate with any of the sensitization parameters, i.e. SPT-sens, nasal provocation titre, absolute and relative IgE antibody concentration or CD-sens. CD-sens could be used to monitor omalizumab treatment efficacy while, based on CD-max, four of seven symptom-free patients on omalizumab would have been classified as having ongoing allergy. Conclusions: CD-sens seems to be very useful for the determination of a patient's allergen sensitivity and should be evaluated for the measurement and monitoring of anti-IgE treatment efficacy. CD-max, the conventional approach to basophil allergen challenge, which mirrors cell reactivity, gives incorrect information. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
omalizumab, immunoglobulin E, CD63, CD-sens, CD203c
in
Allergy
volume
61
issue
3
pages
298 - 302
publisher
Wiley-Blackwell
external identifiers
  • pmid:16436137
  • wos:000234853900005
  • scopus:33644903489
  • pmid:16436137
ISSN
1398-9995
DOI
10.1111/j.1398-9995.2006.00987.x
language
English
LU publication?
yes
id
5f110f19-8744-458d-88f8-4d0178ad97f6 (old id 419580)
date added to LUP
2016-04-01 16:32:30
date last changed
2021-10-06 04:36:46
@article{5f110f19-8744-458d-88f8-4d0178ad97f6,
  abstract     = {Background: Monitoring of the allergen sensitivity of a patient is most important for optimal patient care and a basic prerequisite for immunomodulating treatment. The objective of this study was to investigate how basophil allergen sensitivity can be applied in the monitoring of anti-immunoglobulin E (IgE) treatment. Methods: Basophils from timothy grass pollen allergic patients were, by flow cytometry, analysed for allergen threshold sensitivity (CD-sens) by measuring CD63 up-regulation on CD203c-identified basophils. The results were compared with maximal percentage CD63 up-regulation at one allergen dose (CD-max), skin prick test end-point allergen titration, (SPT-sens), nasal provocation titration tests (nasal provocation titre) and serum IgE and IgE antibody concentrations. Results: There was a significant correlation (r = 0.50, P = 0.01) between CD-sens and SPT-sens, CD-sens and the IgE antibody concentration in percentage of 'total IgE' (relative IgE antibody concentration) (r = 0.72, P &lt; 0.001) as well as between CD-sens and nasal provocation titre (r = 0.54, P &lt; 0.05) but, in contrast, CD-max did not correlate with any of the sensitization parameters, i.e. SPT-sens, nasal provocation titre, absolute and relative IgE antibody concentration or CD-sens. CD-sens could be used to monitor omalizumab treatment efficacy while, based on CD-max, four of seven symptom-free patients on omalizumab would have been classified as having ongoing allergy. Conclusions: CD-sens seems to be very useful for the determination of a patient's allergen sensitivity and should be evaluated for the measurement and monitoring of anti-IgE treatment efficacy. CD-max, the conventional approach to basophil allergen challenge, which mirrors cell reactivity, gives incorrect information.},
  author       = {Nopp, A and Johansson, SGO and Ankerst, Jaro and Bylin, G and Cardell, Lars-Olaf and Gronneberg, R and Irander, K and Palmqvist, M and Oman, H},
  issn         = {1398-9995},
  language     = {eng},
  number       = {3},
  pages        = {298--302},
  publisher    = {Wiley-Blackwell},
  series       = {Allergy},
  title        = {Basophil allergen threshold sensitivity: a useful approach to anti-IgE treatment efficacy evaluation},
  url          = {http://dx.doi.org/10.1111/j.1398-9995.2006.00987.x},
  doi          = {10.1111/j.1398-9995.2006.00987.x},
  volume       = {61},
  year         = {2006},
}