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Automated flow-injection immunosensor based on current pulse capacitive measurements

Erlandsson, Dag; Teeparuksapun, Kosin LU ; Mattiasson, Bo LU and Hedström, Martin LU (2014) In Sensors and Actuators B: Chemical 190. p.295-304
Abstract
This document describes a new concept for assessing capacitance based on a constant current pulse to the biosensor transducer. The biosensor has a working electrode that is coated with an insulating molecular layer including a ligand which forms an affinity surface. A sensor electrode is brought into contact with electrolyte solution, and the new measuring principle then involves steps where three different constant currents (I-1, I-2 and I-3) are serially pulsed on the sensor surface during pre-determined time periods. The potential that is built up (rising) across the sensor surface is sampled every 6.8 mu s. The inclination of the registered potential profile corresponding to the current pulsed was utilized to calculate both capacitance... (More)
This document describes a new concept for assessing capacitance based on a constant current pulse to the biosensor transducer. The biosensor has a working electrode that is coated with an insulating molecular layer including a ligand which forms an affinity surface. A sensor electrode is brought into contact with electrolyte solution, and the new measuring principle then involves steps where three different constant currents (I-1, I-2 and I-3) are serially pulsed on the sensor surface during pre-determined time periods. The potential that is built up (rising) across the sensor surface is sampled every 6.8 mu s. The inclination of the registered potential profile corresponding to the current pulsed was utilized to calculate both capacitance and resistance. The new current-based measurement method shows a 10-fold increase in stability for the capacitive measurement as compared to the potential pulse technique. Quantitation of HIV-1 p24 using monoclonal anti-HIV-1 p24 antibodies was used as a model system for the evaluation of the technique. The binding of HIV-1 p24 antigens to the immobilized antibodies causes the capacitance to decrease. The change in capacitance was proportional to the concentration of HIV-1 p24. The capacitance measurement using the current pulse method offers a stable sensing technique with a broad range of potential applications. (C) 2013 Elsevier B. V. All rights reserved. (Less)
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author
organization
publishing date
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Contribution to journal
publication status
published
subject
keywords
Current pulse, Capacitive, Automated flow-injection, HIV-1 p24
in
Sensors and Actuators B: Chemical
volume
190
pages
295 - 304
publisher
Elsevier
external identifiers
  • wos:000326687700039
  • scopus:84884678128
ISSN
0925-4005
DOI
10.1016/j.snb.2013.08.076
language
English
LU publication?
yes
id
f86869f3-427f-401d-b82a-319e7ff95fd2 (old id 4197876)
date added to LUP
2014-01-13 14:18:54
date last changed
2017-07-30 04:23:51
@article{f86869f3-427f-401d-b82a-319e7ff95fd2,
  abstract     = {This document describes a new concept for assessing capacitance based on a constant current pulse to the biosensor transducer. The biosensor has a working electrode that is coated with an insulating molecular layer including a ligand which forms an affinity surface. A sensor electrode is brought into contact with electrolyte solution, and the new measuring principle then involves steps where three different constant currents (I-1, I-2 and I-3) are serially pulsed on the sensor surface during pre-determined time periods. The potential that is built up (rising) across the sensor surface is sampled every 6.8 mu s. The inclination of the registered potential profile corresponding to the current pulsed was utilized to calculate both capacitance and resistance. The new current-based measurement method shows a 10-fold increase in stability for the capacitive measurement as compared to the potential pulse technique. Quantitation of HIV-1 p24 using monoclonal anti-HIV-1 p24 antibodies was used as a model system for the evaluation of the technique. The binding of HIV-1 p24 antigens to the immobilized antibodies causes the capacitance to decrease. The change in capacitance was proportional to the concentration of HIV-1 p24. The capacitance measurement using the current pulse method offers a stable sensing technique with a broad range of potential applications. (C) 2013 Elsevier B. V. All rights reserved.},
  author       = {Erlandsson, Dag and Teeparuksapun, Kosin and Mattiasson, Bo and Hedström, Martin},
  issn         = {0925-4005},
  keyword      = {Current pulse,Capacitive,Automated flow-injection,HIV-1 p24},
  language     = {eng},
  pages        = {295--304},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Automated flow-injection immunosensor based on current pulse capacitive measurements},
  url          = {http://dx.doi.org/10.1016/j.snb.2013.08.076},
  volume       = {190},
  year         = {2014},
}