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Biotransformation of glycerol to 3-hydroxypropionaldehyde: Improved production by in situ complexation with bisulfite in a fed-batch mode and separation on anion exchanger.

Sardari, Roya; Dishisha, Tarek LU ; Pyo, Sang-Hyun LU and Hatti-Kaul, Rajni LU (2013) In Journal of Biotechnology 168(4). p.534-542
Abstract
3-Hydroxypropionaldehyde (3HPA) is an important C3 chemical that can be produced from renewable glycerol by resting whole cells of Lactobacillus reuteri. However the process efficiency is limited due to substrate inhibition, product-mediated loss of enzyme activity and cell viability, and also formation of by-products. Complex formation of 3HPA with sodium bisulfite and subsequent binding to Amberlite IRA-400 was investigated as a means of in situ product recovery and for overcoming inhibition. The adsorption capacity and -isotherm of the resin were evaluated using the Langmuir model. The resin exhibited maximum capacity of 2.92mmol complex/g when equilibrated with 45mL solution containing an equilibrium mixture of 2.74mmol 3HPA-bisulfite... (More)
3-Hydroxypropionaldehyde (3HPA) is an important C3 chemical that can be produced from renewable glycerol by resting whole cells of Lactobacillus reuteri. However the process efficiency is limited due to substrate inhibition, product-mediated loss of enzyme activity and cell viability, and also formation of by-products. Complex formation of 3HPA with sodium bisulfite and subsequent binding to Amberlite IRA-400 was investigated as a means of in situ product recovery and for overcoming inhibition. The adsorption capacity and -isotherm of the resin were evaluated using the Langmuir model. The resin exhibited maximum capacity of 2.92mmol complex/g when equilibrated with 45mL solution containing an equilibrium mixture of 2.74mmol 3HPA-bisulfite complex and 2.01mmol free 3HPA. The dynamic binding capacity based on the breakthrough curve of 3HPA and its complex on passing a solution with 2.49mmol complex and 1.65mmol free 3HPA was 2.01mmol/g resin. The bound 3HPA was desorbed from the resin using 0.20M NaCl with a high purity as a mixture of complexed- and free 3HPA at a ratio of 0.77mol/mol. Fed-batch biotransformation of glycerol (818.85mmol) with in situ 3HPA complexation and separation on the bisulfite-functionalized resin resulted in an improved process with consumption of 481.36mmol glycerol yielding 325.54mmol 3HPA at a rate of 17.13mmol/h and a yield of 68 mol%. Also, the cell activity was maintained for at least 28h. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biotechnology
volume
168
issue
4
pages
534 - 542
publisher
Elsevier
external identifiers
  • wos:000327843200034
  • pmid:24060827
  • scopus:84888836289
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2013.09.009
language
English
LU publication?
yes
id
41d4a71d-63c1-447f-ba7f-36a368cba5e0 (old id 4065467)
date added to LUP
2013-11-13 17:17:59
date last changed
2019-02-20 01:21:25
@article{41d4a71d-63c1-447f-ba7f-36a368cba5e0,
  abstract     = {3-Hydroxypropionaldehyde (3HPA) is an important C3 chemical that can be produced from renewable glycerol by resting whole cells of Lactobacillus reuteri. However the process efficiency is limited due to substrate inhibition, product-mediated loss of enzyme activity and cell viability, and also formation of by-products. Complex formation of 3HPA with sodium bisulfite and subsequent binding to Amberlite IRA-400 was investigated as a means of in situ product recovery and for overcoming inhibition. The adsorption capacity and -isotherm of the resin were evaluated using the Langmuir model. The resin exhibited maximum capacity of 2.92mmol complex/g when equilibrated with 45mL solution containing an equilibrium mixture of 2.74mmol 3HPA-bisulfite complex and 2.01mmol free 3HPA. The dynamic binding capacity based on the breakthrough curve of 3HPA and its complex on passing a solution with 2.49mmol complex and 1.65mmol free 3HPA was 2.01mmol/g resin. The bound 3HPA was desorbed from the resin using 0.20M NaCl with a high purity as a mixture of complexed- and free 3HPA at a ratio of 0.77mol/mol. Fed-batch biotransformation of glycerol (818.85mmol) with in situ 3HPA complexation and separation on the bisulfite-functionalized resin resulted in an improved process with consumption of 481.36mmol glycerol yielding 325.54mmol 3HPA at a rate of 17.13mmol/h and a yield of 68 mol%. Also, the cell activity was maintained for at least 28h.},
  author       = {Sardari, Roya and Dishisha, Tarek and Pyo, Sang-Hyun and Hatti-Kaul, Rajni},
  issn         = {1873-4863},
  language     = {eng},
  number       = {4},
  pages        = {534--542},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Biotransformation of glycerol to 3-hydroxypropionaldehyde: Improved production by in situ complexation with bisulfite in a fed-batch mode and separation on anion exchanger.},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2013.09.009},
  volume       = {168},
  year         = {2013},
}