Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Characterization of chemical substitution of hydroxypropyl cellulose using enzymatic degradation

Schagerlöf, Herje LU ; Richardson, S ; Momcilovic, D ; Brinkmalm, G ; Wittgren, B and Tjerneld, Folke LU (2006) In Biomacromolecules 7(1). p.80-85
Abstract
The distribution of substituents along the polymer backbone will have a strong influence on the properties of modified cellulose. Endoglucanases were used to degrade a series of hydroxypropyl cellulose (HPC) derivatives with a high degree of substitution. The HPCs were characterized with cloud-point analysis prior to degradation. The extent of enzymatic degradation was determined with size-exclusion chromatography with online multi-angle light scattering and refractive index detection and also with high-pH anion exchange chromatography with pulsed amperometric detection. To further characterize the formed products, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was employed for analysis of short-chained... (More)
The distribution of substituents along the polymer backbone will have a strong influence on the properties of modified cellulose. Endoglucanases were used to degrade a series of hydroxypropyl cellulose (HPC) derivatives with a high degree of substitution. The HPCs were characterized with cloud-point analysis prior to degradation. The extent of enzymatic degradation was determined with size-exclusion chromatography with online multi-angle light scattering and refractive index detection and also with high-pH anion exchange chromatography with pulsed amperometric detection. To further characterize the formed products, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was employed for analysis of short-chained oligosaccharides. The different endoglucanases showed varying degradation capability depending on structure of the active site. The highly substituted HPCs had different susceptibility to degradation by the endoglucanases. The results show a difference in substituent distribution between HPCs, which would explain the differing cloud-point behaviors. Increased number of regions with low substitution could be, correlated with lower polymer cloud point. The study shows the usefulness of enzymatic degradation to study the distribution of substituents in soluble biopolymer derivates. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
7
issue
1
pages
80 - 85
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:16398501
  • wos:000234549100014
  • scopus:31544442935
  • pmid:16398501
ISSN
1526-4602
DOI
10.1021/bm050430n
language
English
LU publication?
yes
id
b3a66b3b-247e-46d4-8c5f-4f85d9ceab53 (old id 421434)
date added to LUP
2016-04-01 12:04:01
date last changed
2020-02-19 01:54:28
@article{b3a66b3b-247e-46d4-8c5f-4f85d9ceab53,
  abstract     = {The distribution of substituents along the polymer backbone will have a strong influence on the properties of modified cellulose. Endoglucanases were used to degrade a series of hydroxypropyl cellulose (HPC) derivatives with a high degree of substitution. The HPCs were characterized with cloud-point analysis prior to degradation. The extent of enzymatic degradation was determined with size-exclusion chromatography with online multi-angle light scattering and refractive index detection and also with high-pH anion exchange chromatography with pulsed amperometric detection. To further characterize the formed products, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was employed for analysis of short-chained oligosaccharides. The different endoglucanases showed varying degradation capability depending on structure of the active site. The highly substituted HPCs had different susceptibility to degradation by the endoglucanases. The results show a difference in substituent distribution between HPCs, which would explain the differing cloud-point behaviors. Increased number of regions with low substitution could be, correlated with lower polymer cloud point. The study shows the usefulness of enzymatic degradation to study the distribution of substituents in soluble biopolymer derivates.},
  author       = {Schagerlöf, Herje and Richardson, S and Momcilovic, D and Brinkmalm, G and Wittgren, B and Tjerneld, Folke},
  issn         = {1526-4602},
  language     = {eng},
  number       = {1},
  pages        = {80--85},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Biomacromolecules},
  title        = {Characterization of chemical substitution of hydroxypropyl cellulose using enzymatic degradation},
  url          = {http://dx.doi.org/10.1021/bm050430n},
  doi          = {10.1021/bm050430n},
  volume       = {7},
  year         = {2006},
}