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A mathematical model of the euglycemic hyperinsulinemic clamp

Picchini, Umberto LU ; De Gaetano, Andrea; Panunzi, Simona; Ditlevsen, Susanne and Mingrone, Geltrude (2005) In Theoretical Biology Medical Modelling 2(44).
Abstract
Background

The Euglycemic Hyperinsulinemic Clamp (EHC) is the most widely used experimental procedure for the determination of insulin sensitivity, and in its usual form the patient is followed under insulinization for two hours. In the present study, sixteen subjects with BMI between 18.5 and 63.6 kg/m2 were studied by long-duration (five hours) EHC.



Results

From the results of this series and from similar reports in the literature it is clear that, in obese subjects, glucose uptake rates continue to increase if the clamp procedure is prolonged beyond the customary 2 hours. A mathematical model of the EHC, incorporating delays, was fitted to the recorded data, and the insulin resistance behaviour of... (More)
Background

The Euglycemic Hyperinsulinemic Clamp (EHC) is the most widely used experimental procedure for the determination of insulin sensitivity, and in its usual form the patient is followed under insulinization for two hours. In the present study, sixteen subjects with BMI between 18.5 and 63.6 kg/m2 were studied by long-duration (five hours) EHC.



Results

From the results of this series and from similar reports in the literature it is clear that, in obese subjects, glucose uptake rates continue to increase if the clamp procedure is prolonged beyond the customary 2 hours. A mathematical model of the EHC, incorporating delays, was fitted to the recorded data, and the insulin resistance behaviour of obese subjects was assessed analytically. Obese subjects had significantly less effective suppression of hepatic glucose output and higher pancreatic insulin secretion than lean subjects. Tissue insulin resistance appeared to be higher in the obese group, but this difference did not reach statistical significance.



Conclusion

The use of a mathematical model allows a greater amount of information to be recovered from clamp data, making it easier to understand the components of insulin resistance in obese vs. normal subjects. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Theoretical Biology Medical Modelling
volume
2
issue
44
publisher
BioMed Central
external identifiers
  • scopus:28444441579
ISSN
1742-4682
DOI
10.1186/1742-4682-2-44
language
English
LU publication?
no
id
52308f78-81ed-4593-9999-3aefc7d69646 (old id 4216019)
date added to LUP
2014-01-13 13:52:01
date last changed
2017-07-30 04:43:05
@article{52308f78-81ed-4593-9999-3aefc7d69646,
  abstract     = {Background<br/><br>
The Euglycemic Hyperinsulinemic Clamp (EHC) is the most widely used experimental procedure for the determination of insulin sensitivity, and in its usual form the patient is followed under insulinization for two hours. In the present study, sixteen subjects with BMI between 18.5 and 63.6 kg/m2 were studied by long-duration (five hours) EHC.<br/><br>
<br/><br>
Results<br/><br>
From the results of this series and from similar reports in the literature it is clear that, in obese subjects, glucose uptake rates continue to increase if the clamp procedure is prolonged beyond the customary 2 hours. A mathematical model of the EHC, incorporating delays, was fitted to the recorded data, and the insulin resistance behaviour of obese subjects was assessed analytically. Obese subjects had significantly less effective suppression of hepatic glucose output and higher pancreatic insulin secretion than lean subjects. Tissue insulin resistance appeared to be higher in the obese group, but this difference did not reach statistical significance.<br/><br>
<br/><br>
Conclusion<br/><br>
The use of a mathematical model allows a greater amount of information to be recovered from clamp data, making it easier to understand the components of insulin resistance in obese vs. normal subjects.},
  author       = {Picchini, Umberto and De Gaetano, Andrea and Panunzi, Simona and Ditlevsen, Susanne and Mingrone, Geltrude},
  issn         = {1742-4682},
  language     = {eng},
  number       = {44},
  publisher    = {BioMed Central},
  series       = {Theoretical Biology Medical Modelling},
  title        = {A mathematical model of the euglycemic hyperinsulinemic clamp},
  url          = {http://dx.doi.org/10.1186/1742-4682-2-44},
  volume       = {2},
  year         = {2005},
}