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Purification, crystallization and X-ray diffraction analysis of dihydropyrimidinase from Dictyostelium discoideum

Lohkamp, B ; Andersen, Birgit LU ; Piskur, Jure LU and Dobritzsch, D (2006) In Acta Crystallographica. Section F: Structural Biology and Crystallization Communications 62(1). p.36-38
Abstract
Dihydropyrimidinase (EC 3.5.2.2) is the second enzyme in the reductive pyrimidine-degradation pathway and catalyses the hydrolysis of 5,6-dihydrouracil and 5,6-dihydrothymine to the corresponding N-carbamylated beta-amino acids. The recombinant enzyme from the slime mould Dictyostelium discoideum was overexpressed, purified and crystallized by the vapour-diffusion method. One crystal diffracted to better than 1.8 angstrom resolution on a synchrotron source and was shown to belong to space group I222, with unit-cell parameters a = 84.6, b = 89.6, c = 134.9 angstrom and one molecule in the asymmetric unit.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
volume
62
issue
1
pages
36 - 38
publisher
Wiley-Blackwell
external identifiers
  • pmid:16511257
  • wos:000234169100011
  • scopus:33646860795
ISSN
2053-230X
DOI
10.1107/s174430910503976x
language
English
LU publication?
yes
id
abddacff-188a-402a-952c-9025759ad191 (old id 421719)
date added to LUP
2016-04-01 16:19:36
date last changed
2022-01-28 18:55:51
@article{abddacff-188a-402a-952c-9025759ad191,
  abstract     = {{Dihydropyrimidinase (EC 3.5.2.2) is the second enzyme in the reductive pyrimidine-degradation pathway and catalyses the hydrolysis of 5,6-dihydrouracil and 5,6-dihydrothymine to the corresponding N-carbamylated beta-amino acids. The recombinant enzyme from the slime mould Dictyostelium discoideum was overexpressed, purified and crystallized by the vapour-diffusion method. One crystal diffracted to better than 1.8 angstrom resolution on a synchrotron source and was shown to belong to space group I222, with unit-cell parameters a = 84.6, b = 89.6, c = 134.9 angstrom and one molecule in the asymmetric unit.}},
  author       = {{Lohkamp, B and Andersen, Birgit and Piskur, Jure and Dobritzsch, D}},
  issn         = {{2053-230X}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{36--38}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Acta Crystallographica. Section F: Structural Biology and Crystallization Communications}},
  title        = {{Purification, crystallization and X-ray diffraction analysis of dihydropyrimidinase from Dictyostelium discoideum}},
  url          = {{http://dx.doi.org/10.1107/s174430910503976x}},
  doi          = {{10.1107/s174430910503976x}},
  volume       = {{62}},
  year         = {{2006}},
}