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Mineralisation of soft and hard tissues and the stability of biofluids.

Holt, Carl; Lenton, Samuel; Nylander, Tommy LU ; Sørensen, Esben S and Teixeira, Susana C M (2014) In Journal of Structural Biology 185(3). p.383-396
Abstract
Evidence is provided from studies on natural and artificial biofluids that the sequestration of amorphous calcium phosphate by peptides or proteins to form nanocluster complexes is of general importance in the control of physiological calcification. A naturally occurring mixture of osteopontin peptides was shown, by light and neutron scattering, to form calcium phosphate nanoclusters with a core-shell structure. In blood serum and stimulated saliva, an invariant calcium phosphate ion activity product was found which corresponds closely in form and magnitude to the ion activity product observed in solutions of these osteopontin nanoclusters. This suggests that types of nanocluster complexes are present in these biofluids as well as in milk.... (More)
Evidence is provided from studies on natural and artificial biofluids that the sequestration of amorphous calcium phosphate by peptides or proteins to form nanocluster complexes is of general importance in the control of physiological calcification. A naturally occurring mixture of osteopontin peptides was shown, by light and neutron scattering, to form calcium phosphate nanoclusters with a core-shell structure. In blood serum and stimulated saliva, an invariant calcium phosphate ion activity product was found which corresponds closely in form and magnitude to the ion activity product observed in solutions of these osteopontin nanoclusters. This suggests that types of nanocluster complexes are present in these biofluids as well as in milk. Precipitation of amorphous calcium phosphate from artificial blood serum, urine and saliva was determined as a function of pH and the concentration of osteopontin or casein phosphopeptides. The position of the boundary between stability and precipitation was found to agree quantitatively with the theory of nanocluster formation. Artificial biofluids were prepared that closely matched their natural counterparts in calcium and phosphate concentrations, pH, saturation, ionic strength and osmolality. Such fluids, stabilised by a low concentration of sequestering phosphopeptides, were found to be highly stable and may have a number of beneficial applications in medicine. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Structural Biology
volume
185
issue
3
pages
383 - 396
publisher
Elsevier
external identifiers
  • pmid:24316224
  • wos:000332815400017
  • scopus:84894504806
ISSN
1095-8657
DOI
10.1016/j.jsb.2013.11.009
language
English
LU publication?
yes
id
f7ec5a03-2cb2-47f1-8bd9-d8ff8bf6a336 (old id 4225041)
date added to LUP
2014-02-05 17:46:23
date last changed
2017-05-21 03:04:24
@article{f7ec5a03-2cb2-47f1-8bd9-d8ff8bf6a336,
  abstract     = {Evidence is provided from studies on natural and artificial biofluids that the sequestration of amorphous calcium phosphate by peptides or proteins to form nanocluster complexes is of general importance in the control of physiological calcification. A naturally occurring mixture of osteopontin peptides was shown, by light and neutron scattering, to form calcium phosphate nanoclusters with a core-shell structure. In blood serum and stimulated saliva, an invariant calcium phosphate ion activity product was found which corresponds closely in form and magnitude to the ion activity product observed in solutions of these osteopontin nanoclusters. This suggests that types of nanocluster complexes are present in these biofluids as well as in milk. Precipitation of amorphous calcium phosphate from artificial blood serum, urine and saliva was determined as a function of pH and the concentration of osteopontin or casein phosphopeptides. The position of the boundary between stability and precipitation was found to agree quantitatively with the theory of nanocluster formation. Artificial biofluids were prepared that closely matched their natural counterparts in calcium and phosphate concentrations, pH, saturation, ionic strength and osmolality. Such fluids, stabilised by a low concentration of sequestering phosphopeptides, were found to be highly stable and may have a number of beneficial applications in medicine.},
  author       = {Holt, Carl and Lenton, Samuel and Nylander, Tommy and Sørensen, Esben S and Teixeira, Susana C M},
  issn         = {1095-8657},
  language     = {eng},
  number       = {3},
  pages        = {383--396},
  publisher    = {Elsevier},
  series       = {Journal of Structural Biology},
  title        = {Mineralisation of soft and hard tissues and the stability of biofluids.},
  url          = {http://dx.doi.org/10.1016/j.jsb.2013.11.009},
  volume       = {185},
  year         = {2014},
}