Low density lipoprotein induces upregulation of vasoconstrictive endothelin type B receptor expression.
(2014) In Vascular Pharmacology 60(1). p.42-48- Abstract
- Vasoconstrictive endothelin type B (ETB) receptors promote vasospasm and ischemic cerebro- and cardiovascular diseases. The present study was designed to examine if low density lipoprotein (LDL) induces upregulation of vasoconstrictive ETB receptor expression and if extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) signal pathways are involved in this process. Rat mesenteric artery segments were organ cultured in the presence and absence of LDL with or without inhibitors for MAPK kinase 1 and 2 (MEK1/2), p38 and transcription. The upregulation of vasoconstrictive ETB receptor expression was studied using a sensitive myograph, real-time PCR and Western blot. LDL (11, 22 and 44mg... (More)
- Vasoconstrictive endothelin type B (ETB) receptors promote vasospasm and ischemic cerebro- and cardiovascular diseases. The present study was designed to examine if low density lipoprotein (LDL) induces upregulation of vasoconstrictive ETB receptor expression and if extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) signal pathways are involved in this process. Rat mesenteric artery segments were organ cultured in the presence and absence of LDL with or without inhibitors for MAPK kinase 1 and 2 (MEK1/2), p38 and transcription. The upregulation of vasoconstrictive ETB receptor expression was studied using a sensitive myograph, real-time PCR and Western blot. LDL (11, 22 and 44mg protein/L) concentration-dependently induced upregulation of vasoconstrictive ETB receptor expression with increase in the receptor-mediated vasoconstriction, elevated levels of the ETB receptor mRNA and protein expressions, and activation of ERK1/2 and p38 MAPK. Blockage of ERK1/2 and p38 MAPK signal pathways using MEK1/2 inhibitors (PD98059 and U0126) or p38 inhibitors (SB203580 and SB239063) significantly abolished the LDL-induced upregulation of vasoconstrictive ETB receptor expression. Actinomycin D (general transcriptional inhibitor) almost completely inhibited the LDL effects. In conclusion, LDL induces upregulation of vasoconstrictive ETB receptor expression through activation of ERK1/2 and p38 MAPK signal pathway-dependent transcriptional mechanisms. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4225057
- author
- Xu, Cang-Bao LU ; Zheng, Jianpu LU ; Zhang, Wei LU ; Liu, Enqi ; Edvinsson, Lars LU and Zhang, Yaping LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Vascular Pharmacology
- volume
- 60
- issue
- 1
- pages
- 42 - 48
- publisher
- Elsevier
- external identifiers
-
- pmid:24315856
- wos:000330600100006
- scopus:84892493929
- pmid:24315856
- ISSN
- 1537-1891
- DOI
- 10.1016/j.vph.2013.11.004
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medicine (Lund) (013230025), Chemical Physics (S) (011001060)
- id
- 603afb83-2ca0-4754-a0f6-7d3244008a43 (old id 4225057)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24315856?dopt=Abstract
- date added to LUP
- 2016-04-01 10:16:25
- date last changed
- 2024-01-06 12:27:00
@article{603afb83-2ca0-4754-a0f6-7d3244008a43, abstract = {{Vasoconstrictive endothelin type B (ETB) receptors promote vasospasm and ischemic cerebro- and cardiovascular diseases. The present study was designed to examine if low density lipoprotein (LDL) induces upregulation of vasoconstrictive ETB receptor expression and if extracellular signal-regulated kinases 1 and 2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) signal pathways are involved in this process. Rat mesenteric artery segments were organ cultured in the presence and absence of LDL with or without inhibitors for MAPK kinase 1 and 2 (MEK1/2), p38 and transcription. The upregulation of vasoconstrictive ETB receptor expression was studied using a sensitive myograph, real-time PCR and Western blot. LDL (11, 22 and 44mg protein/L) concentration-dependently induced upregulation of vasoconstrictive ETB receptor expression with increase in the receptor-mediated vasoconstriction, elevated levels of the ETB receptor mRNA and protein expressions, and activation of ERK1/2 and p38 MAPK. Blockage of ERK1/2 and p38 MAPK signal pathways using MEK1/2 inhibitors (PD98059 and U0126) or p38 inhibitors (SB203580 and SB239063) significantly abolished the LDL-induced upregulation of vasoconstrictive ETB receptor expression. Actinomycin D (general transcriptional inhibitor) almost completely inhibited the LDL effects. In conclusion, LDL induces upregulation of vasoconstrictive ETB receptor expression through activation of ERK1/2 and p38 MAPK signal pathway-dependent transcriptional mechanisms.}}, author = {{Xu, Cang-Bao and Zheng, Jianpu and Zhang, Wei and Liu, Enqi and Edvinsson, Lars and Zhang, Yaping}}, issn = {{1537-1891}}, language = {{eng}}, number = {{1}}, pages = {{42--48}}, publisher = {{Elsevier}}, series = {{Vascular Pharmacology}}, title = {{Low density lipoprotein induces upregulation of vasoconstrictive endothelin type B receptor expression.}}, url = {{https://lup.lub.lu.se/search/files/1703788/4437311.pdf}}, doi = {{10.1016/j.vph.2013.11.004}}, volume = {{60}}, year = {{2014}}, }