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Inflammatory Markers in Huntington’s Disease Plasma – A Robust NanoLC-MRM-MS Assay Development

Rezeli, Melinda LU ; Végvári, Ákos LU ; Silajdzic, Edina LU ; Björkqvist, Maria LU ; Tabrizi, Sarah; Laurell, Thomas LU and Marko-Varga, György LU (2014) In EuPA Open Proteomics 3. p.68-75
Abstract
The development of a quantitative multiplex reaction monitoring (MRM) assay is presented for the measurements of seven inflammatory markers in human plasma, using nanoLC-MS/MS analysis with stable isotope dilution strategy. We report a robust and sensitive, mass spectrometry based, quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing, while the analytical reproducibility of the assay was 3.6%. We used plasma from Huntington´s disease (HD) gene carriers and healthy controls to compare our MRM method with established antibody based methods. Interestingly, we found a good agreement between assays for the measurement of C-reactive protein (CRP).
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
multiple reaction monitoring, complement components, C-reactive protein, Huntington´s disease
in
EuPA Open Proteomics
volume
3
pages
68 - 75
publisher
Elsevier
external identifiers
  • scopus:84902440223
ISSN
2212-9685
DOI
10.1016/j.euprot.2014.02.003
language
English
LU publication?
yes
id
9cbc16b1-88d5-4ad8-b58e-9b38d282b6db (old id 4227339)
date added to LUP
2014-04-04 12:55:14
date last changed
2017-01-01 06:32:22
@article{9cbc16b1-88d5-4ad8-b58e-9b38d282b6db,
  abstract     = {The development of a quantitative multiplex reaction monitoring (MRM) assay is presented for the measurements of seven inflammatory markers in human plasma, using nanoLC-MS/MS analysis with stable isotope dilution strategy. We report a robust and sensitive, mass spectrometry based, quantitative assay with a relative standard deviation of &lt;15% that accounts for the entire sample processing, while the analytical reproducibility of the assay was 3.6%. We used plasma from Huntington´s disease (HD) gene carriers and healthy controls to compare our MRM method with established antibody based methods. Interestingly, we found a good agreement between assays for the measurement of C-reactive protein (CRP).},
  author       = {Rezeli, Melinda and Végvári, Ákos and Silajdzic, Edina and Björkqvist, Maria and Tabrizi, Sarah and Laurell, Thomas and Marko-Varga, György},
  issn         = {2212-9685},
  keyword      = {multiple reaction monitoring,complement components,C-reactive protein,Huntington´s disease},
  language     = {eng},
  pages        = {68--75},
  publisher    = {Elsevier},
  series       = {EuPA Open Proteomics},
  title        = {Inflammatory Markers in Huntington’s Disease Plasma – A Robust NanoLC-MRM-MS Assay Development},
  url          = {http://dx.doi.org/10.1016/j.euprot.2014.02.003},
  volume       = {3},
  year         = {2014},
}