Inflammatory Markers in Huntington’s Disease Plasma – A Robust NanoLC-MRM-MS Assay Development
(2014) In EuPA Open Proteomics 3. p.68-75- Abstract
- The development of a quantitative multiplex reaction monitoring (MRM) assay is presented for the measurements of seven inflammatory markers in human plasma, using nanoLC-MS/MS analysis with stable isotope dilution strategy. We report a robust and sensitive, mass spectrometry based, quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing, while the analytical reproducibility of the assay was 3.6%. We used plasma from Huntington´s disease (HD) gene carriers and healthy controls to compare our MRM method with established antibody based methods. Interestingly, we found a good agreement between assays for the measurement of C-reactive protein (CRP).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4227339
- author
- Rezeli, Melinda
LU
; Végvári, Ákos LU ; Silajdzic, Edina LU ; Björkqvist, Maria LU
; Tabrizi, Sarah ; Laurell, Thomas LU and Marko-Varga, György LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- multiple reaction monitoring, complement components, C-reactive protein, Huntington´s disease
- in
- EuPA Open Proteomics
- volume
- 3
- pages
- 68 - 75
- publisher
- Elsevier
- external identifiers
-
- scopus:84902440223
- ISSN
- 2212-9685
- DOI
- 10.1016/j.euprot.2014.02.003
- language
- English
- LU publication?
- yes
- id
- 9cbc16b1-88d5-4ad8-b58e-9b38d282b6db (old id 4227339)
- date added to LUP
- 2016-04-01 14:58:58
- date last changed
- 2023-09-03 21:42:13
@article{9cbc16b1-88d5-4ad8-b58e-9b38d282b6db, abstract = {{The development of a quantitative multiplex reaction monitoring (MRM) assay is presented for the measurements of seven inflammatory markers in human plasma, using nanoLC-MS/MS analysis with stable isotope dilution strategy. We report a robust and sensitive, mass spectrometry based, quantitative assay with a relative standard deviation of <15% that accounts for the entire sample processing, while the analytical reproducibility of the assay was 3.6%. We used plasma from Huntington´s disease (HD) gene carriers and healthy controls to compare our MRM method with established antibody based methods. Interestingly, we found a good agreement between assays for the measurement of C-reactive protein (CRP).}}, author = {{Rezeli, Melinda and Végvári, Ákos and Silajdzic, Edina and Björkqvist, Maria and Tabrizi, Sarah and Laurell, Thomas and Marko-Varga, György}}, issn = {{2212-9685}}, keywords = {{multiple reaction monitoring; complement components; C-reactive protein; Huntington´s disease}}, language = {{eng}}, pages = {{68--75}}, publisher = {{Elsevier}}, series = {{EuPA Open Proteomics}}, title = {{Inflammatory Markers in Huntington’s Disease Plasma – A Robust NanoLC-MRM-MS Assay Development}}, url = {{http://dx.doi.org/10.1016/j.euprot.2014.02.003}}, doi = {{10.1016/j.euprot.2014.02.003}}, volume = {{3}}, year = {{2014}}, }