Detection of relevant pathogens and contaminants in blood cultures after implementation of single-sampling strategy and initial specimen diversion
Oldberg, Karl LU
; Kahn, Fredrik
LU
; Rasmussen, Magnus
LU
and Walles, John
LU
(2025)
In European Journal of Clinical Microbiology and Infectious Diseases
44(9).
p.2275-2282
- Abstract
- Purpose
Blood culture collection with all bottles filled from a single venipuncture (single-sampling strategy, SSS) is increasingly used. More data on the effects of SSS on blood culture contamination is needed. After combined implementation of SSS and initial specimen diversion (ISDT) in our region, we evaluated the proportion of sampling events with at least four bottles, with growth of relevant pathogens and with growth of coagulase-negative staphylococci (CoNS) before and after the intervention.
Methods
We retrospectively reviewed all blood cultures collected from adults in emergency departments in our region in southern Sweden during six years before and 18 months after the intervention.
Results
Among 117... (More) - Purpose
Blood culture collection with all bottles filled from a single venipuncture (single-sampling strategy, SSS) is increasingly used. More data on the effects of SSS on blood culture contamination is needed. After combined implementation of SSS and initial specimen diversion (ISDT) in our region, we evaluated the proportion of sampling events with at least four bottles, with growth of relevant pathogens and with growth of coagulase-negative staphylococci (CoNS) before and after the intervention.
Methods
We retrospectively reviewed all blood cultures collected from adults in emergency departments in our region in southern Sweden during six years before and 18 months after the intervention.
Results
Among 117 142 sampling events included in the analysis, the proportion where at least four bottles were obtained increased from 87.6 to 92.3% (p < 0.001), while growth of relevant pathogens remained unchanged at 13.6%. In the subset of sampling events with no more than one species, there was a decrease in the proportion with CoNS in 1–2 out of 4 bottles (4.0 to 3.3%, p < 0.001), while events with CoNS in 3–4 out of 4 bottles increased (0.39 to 0.49%, p = 0.029).
Conclusion
We confirm positive effects of SSS and ISDT on adequate sample volume and a net decrease in the proportion of sampling events with growth of CoNS. Conversely, we observed an increase in CoNS in multiple bottles, probably caused by contamination. This possible drawback with SSS has not been reported before and should be studied further. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/42459340-25bf-46bc-90bc-d2d2ff259872
- author
- Oldberg, Karl
LU
; Kahn, Fredrik
LU
; Rasmussen, Magnus
LU
and Walles, John
LU
- organization
- publishing date
- 2025-06-25
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Clinical Microbiology and Infectious Diseases
- volume
- 44
- issue
- 9
- pages
- 2275 - 2282
- publisher
- Springer Science and Business Media B.V.
- external identifiers
-
- scopus:105008960633
- pmid:40560506
- ISSN
- 0934-9723
- DOI
- 10.1007/s10096-025-05196-4
- language
- English
- LU publication?
- yes
- id
- 42459340-25bf-46bc-90bc-d2d2ff259872
- date added to LUP
- 2025-09-22 09:05:23
- date last changed
- 2025-10-21 00:55:49
@article{42459340-25bf-46bc-90bc-d2d2ff259872,
abstract = {{Purpose<br/>Blood culture collection with all bottles filled from a single venipuncture (single-sampling strategy, SSS) is increasingly used. More data on the effects of SSS on blood culture contamination is needed. After combined implementation of SSS and initial specimen diversion (ISDT) in our region, we evaluated the proportion of sampling events with at least four bottles, with growth of relevant pathogens and with growth of coagulase-negative staphylococci (CoNS) before and after the intervention.<br/><br/>Methods<br/>We retrospectively reviewed all blood cultures collected from adults in emergency departments in our region in southern Sweden during six years before and 18 months after the intervention.<br/><br/>Results<br/>Among 117 142 sampling events included in the analysis, the proportion where at least four bottles were obtained increased from 87.6 to 92.3% (p < 0.001), while growth of relevant pathogens remained unchanged at 13.6%. In the subset of sampling events with no more than one species, there was a decrease in the proportion with CoNS in 1–2 out of 4 bottles (4.0 to 3.3%, p < 0.001), while events with CoNS in 3–4 out of 4 bottles increased (0.39 to 0.49%, p = 0.029).<br/><br/>Conclusion<br/>We confirm positive effects of SSS and ISDT on adequate sample volume and a net decrease in the proportion of sampling events with growth of CoNS. Conversely, we observed an increase in CoNS in multiple bottles, probably caused by contamination. This possible drawback with SSS has not been reported before and should be studied further.}},
author = {{Oldberg, Karl and Kahn, Fredrik and Rasmussen, Magnus and Walles, John}},
issn = {{0934-9723}},
language = {{eng}},
month = {{06}},
number = {{9}},
pages = {{2275--2282}},
publisher = {{Springer Science and Business Media B.V.}},
series = {{European Journal of Clinical Microbiology and Infectious Diseases}},
title = {{Detection of relevant pathogens and contaminants in blood cultures after implementation of single-sampling strategy and initial specimen diversion}},
url = {{http://dx.doi.org/10.1007/s10096-025-05196-4}},
doi = {{10.1007/s10096-025-05196-4}},
volume = {{44}},
year = {{2025}},
}