Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Modular 3D printed platform for fluidically connected human brain organoid culture

Rezaei, Babak ; Giacomoni, Jessica LU ; Nilsson, Fredrik LU orcid ; Sozzi, Edoardo LU orcid ; Fiorenzano, Alessandro LU ; Parmar, Malin LU orcid ; Keller, Stephan S. and Kajtez, Janko LU orcid (2024) In Biofabrication 1(15014).
Abstract

Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely on commercially available tools and culturing platforms that had previously been established for different purposes, thus entailing suboptimal culturing conditions and excessive use of plasticware. To address these issues, we developed a 3D printing pipeline for the fabrication of tailor-made culturing platforms for fluidically connected... (More)

Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely on commercially available tools and culturing platforms that had previously been established for different purposes, thus entailing suboptimal culturing conditions and excessive use of plasticware. To address these issues, we developed a 3D printing pipeline for the fabrication of tailor-made culturing platforms for fluidically connected but spatially separated brain organoid array culture. This all-in-one platform allows all culturing steps—from cellular aggregation, spheroid growth, hydrogel embedding, and organoid maturation—to be performed in a single well plate without the need for organoid manipulation or transfer. Importantly, the approach relies on accessible materials and widely available 3D printing equipment. Furthermore, the developed design principles are modular and highly customizable. As such, we believe that the presented technology can be easily adapted by other research groups and fuel further development of culturing tools and platforms for brain organoids and other 3D cellular systems.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3D culture, 3D printing, brain organoids, modular design, neuroscience, stereolithography
in
Biofabrication
volume
1
issue
15014
article number
015014
publisher
IOP Publishing
external identifiers
  • pmid:37956452
  • scopus:85177500554
ISSN
1758-5082
DOI
10.1088/1758-5090/ad0c2c
language
English
LU publication?
yes
additional info
Funding Information: The authors would like to thank Bengt Mattsson for the creation of 3D models and illustrations. Lund University Bioimaging Centre (LBIC) is gratefully acknowledged for providing experimental resources for electron and fluorescence microscopy. B R and S S K acknowledge the financial support from the European Research Council under the Horizon 2020 framework program (Grant No. 772370-PHOENEEX). J K and M P acknowledge the financial support from The Swedish Research Council (2021-02967) and HORIZON-EIC-2021-PATHFINDEROPEN-01 project OpenMIND (101047177). A F is supported by Swedish Research Council (2022-01432) and Italian Ministry of Health (B53C22007860001). Publisher Copyright: © 2023 The Author(s). Published by IOP Publishing Ltd.
id
429f4047-e57a-4f7e-9790-e2bda12d1e12
date added to LUP
2023-12-19 14:41:12
date last changed
2024-04-18 00:57:14
@article{429f4047-e57a-4f7e-9790-e2bda12d1e12,
  abstract     = {{<p>Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely on commercially available tools and culturing platforms that had previously been established for different purposes, thus entailing suboptimal culturing conditions and excessive use of plasticware. To address these issues, we developed a 3D printing pipeline for the fabrication of tailor-made culturing platforms for fluidically connected but spatially separated brain organoid array culture. This all-in-one platform allows all culturing steps—from cellular aggregation, spheroid growth, hydrogel embedding, and organoid maturation—to be performed in a single well plate without the need for organoid manipulation or transfer. Importantly, the approach relies on accessible materials and widely available 3D printing equipment. Furthermore, the developed design principles are modular and highly customizable. As such, we believe that the presented technology can be easily adapted by other research groups and fuel further development of culturing tools and platforms for brain organoids and other 3D cellular systems.</p>}},
  author       = {{Rezaei, Babak and Giacomoni, Jessica and Nilsson, Fredrik and Sozzi, Edoardo and Fiorenzano, Alessandro and Parmar, Malin and Keller, Stephan S. and Kajtez, Janko}},
  issn         = {{1758-5082}},
  keywords     = {{3D culture; 3D printing; brain organoids; modular design; neuroscience; stereolithography}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{15014}},
  publisher    = {{IOP Publishing}},
  series       = {{Biofabrication}},
  title        = {{Modular 3D printed platform for fluidically connected human brain organoid culture}},
  url          = {{http://dx.doi.org/10.1088/1758-5090/ad0c2c}},
  doi          = {{10.1088/1758-5090/ad0c2c}},
  volume       = {{1}},
  year         = {{2024}},
}