Measurement of antihaemophilic factor A antigen (VII:CAg) with a solid phase immunoradiometric method based on homologous non-haemophilic antibodies.
(1979) In Scandinavian Journal of Haematology 23(1). p.17-24- Abstract
Antihaemophilic-factor-A-antibodies, which had spontaneously arisen in 2 patients, were used to develop an immunoradiometric method for measurement of antihaemophilic factor A antigen (VIII:CAg). 13 patients with severe haemophilia A had VIII: CAg below the limit of detection (0.01 U/ml). Patients with moderate and mild haemophilia A either had VIII:CAg roughly equal to factor VIII clotting activity (VIII:C) or a not detectable VIII:CAg, suggesting 2 different molecular mechanisms in moderate and mild haemophilia A. VIII:CAg could be detected in serum but in lower amounts than in plasma. In 2 patients with von Willebrand's disease VIII:CAg equalled VIII:C. The post-transfusional retarded increase of VIII:C in 1 patient with von... (More)
Antihaemophilic-factor-A-antibodies, which had spontaneously arisen in 2 patients, were used to develop an immunoradiometric method for measurement of antihaemophilic factor A antigen (VIII:CAg). 13 patients with severe haemophilia A had VIII: CAg below the limit of detection (0.01 U/ml). Patients with moderate and mild haemophilia A either had VIII:CAg roughly equal to factor VIII clotting activity (VIII:C) or a not detectable VIII:CAg, suggesting 2 different molecular mechanisms in moderate and mild haemophilia A. VIII:CAg could be detected in serum but in lower amounts than in plasma. In 2 patients with von Willebrand's disease VIII:CAg equalled VIII:C. The post-transfusional retarded increase of VIII:C in 1 patient with von Willebrand's disease was accompanied by a slight increase in VIII:CAg. Fetal plasma contained measurable amounts of VIII:CAg.
(Less)
- author
- Holmberg, Lars LU ; Borge, L. ; Ljung, R. LU and Nilsson, Inga Marie
- organization
- publishing date
- 1979
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Haematology
- volume
- 23
- issue
- 1
- pages
- 8 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0018666806
- pmid:91190
- ISSN
- 0036-553X
- language
- English
- LU publication?
- yes
- id
- 42e0e4d7-677a-4ee6-bc58-1610e1e3a0b5
- date added to LUP
- 2016-11-08 14:53:57
- date last changed
- 2024-01-04 15:57:42
@article{42e0e4d7-677a-4ee6-bc58-1610e1e3a0b5, abstract = {{<p>Antihaemophilic-factor-A-antibodies, which had spontaneously arisen in 2 patients, were used to develop an immunoradiometric method for measurement of antihaemophilic factor A antigen (VIII:CAg). 13 patients with severe haemophilia A had VIII: CAg below the limit of detection (0.01 U/ml). Patients with moderate and mild haemophilia A either had VIII:CAg roughly equal to factor VIII clotting activity (VIII:C) or a not detectable VIII:CAg, suggesting 2 different molecular mechanisms in moderate and mild haemophilia A. VIII:CAg could be detected in serum but in lower amounts than in plasma. In 2 patients with von Willebrand's disease VIII:CAg equalled VIII:C. The post-transfusional retarded increase of VIII:C in 1 patient with von Willebrand's disease was accompanied by a slight increase in VIII:CAg. Fetal plasma contained measurable amounts of VIII:CAg.</p>}}, author = {{Holmberg, Lars and Borge, L. and Ljung, R. and Nilsson, Inga Marie}}, issn = {{0036-553X}}, language = {{eng}}, number = {{1}}, pages = {{17--24}}, publisher = {{Wiley-Blackwell}}, series = {{Scandinavian Journal of Haematology}}, title = {{Measurement of antihaemophilic factor A antigen (VII:CAg) with a solid phase immunoradiometric method based on homologous non-haemophilic antibodies.}}, volume = {{23}}, year = {{1979}}, }