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Permanent activation of HMGA2 in lipomas mimics its temporal physiological activation linked to the gain of adipose tissue

Thies, Helge W.; Nolte, Ingo; Wenk, Heiner; Mertens, Fredrik LU ; Bullerdiek, Joern and Markowski, Dominique N. (2014) In Obesity 22(1). p.141-150
Abstract
Objective: In this study the activation of HMGA2 and overexpression by FGF1-driven stimulation of adipose tissue derived stem cells (ADSCs) in adipose tissue tumors were analyzed. In addition, the expression of HMGA2 and PPAR-gamma mRNA were quantified in canine subcutaneous abdominal adipose tissue from normal and overweight purebred dogs. Design and Methods: ADSCs and adipose tissue explants stimulated with FGF1 followed by gene expression analyses of HMGA2 and p14Arf using Western-blot and qRT-PCR. Furthermore, canine subcutaneous white adipose tissue (WAT) were analyzed by qRT-PCR for their expression of HMGA2 and PPAR-gamma. Results: ADSCs and adipose tissue explants are able to execute a HMGA2 response upon FGF1 stimulation. FGF1... (More)
Objective: In this study the activation of HMGA2 and overexpression by FGF1-driven stimulation of adipose tissue derived stem cells (ADSCs) in adipose tissue tumors were analyzed. In addition, the expression of HMGA2 and PPAR-gamma mRNA were quantified in canine subcutaneous abdominal adipose tissue from normal and overweight purebred dogs. Design and Methods: ADSCs and adipose tissue explants stimulated with FGF1 followed by gene expression analyses of HMGA2 and p14Arf using Western-blot and qRT-PCR. Furthermore, canine subcutaneous white adipose tissue (WAT) were analyzed by qRT-PCR for their expression of HMGA2 and PPAR-gamma. Results: ADSCs and adipose tissue explants are able to execute a HMGA2 response upon FGF1 stimulation. FGF1 enhances proliferation of ADSCs by a HMGA2-dependent mechanism. In lipomas increase of HMGA2 is accompanied by increased expression of p14Arf. Furthermore, a significantly elevated level of HMGA2 in overweight dogs and a negative correlation between the expression of HMGA2 and PPAR-gamma in subcutaneous cWAT were noted. Conclusions: These results suggest that WAT contains cells that as essential part of adipogenesis up-regulate HMGA2 resulting from growth factor stimulation. In subgroups of lipoma, constitutive activation of HMGA2 due to rearrangements replaces the temporal response triggered by growth factors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Obesity
volume
22
issue
1
pages
141 - 150
publisher
Nature Publishing Group
external identifiers
  • wos:000329613600023
  • scopus:84892371029
ISSN
1930-739X
DOI
10.1002/oby.20137
language
English
LU publication?
yes
id
ba2b4173-9275-491b-824b-cdb9bb6e1068 (old id 4319666)
date added to LUP
2014-03-03 08:03:17
date last changed
2017-01-01 04:04:09
@article{ba2b4173-9275-491b-824b-cdb9bb6e1068,
  abstract     = {Objective: In this study the activation of HMGA2 and overexpression by FGF1-driven stimulation of adipose tissue derived stem cells (ADSCs) in adipose tissue tumors were analyzed. In addition, the expression of HMGA2 and PPAR-gamma mRNA were quantified in canine subcutaneous abdominal adipose tissue from normal and overweight purebred dogs. Design and Methods: ADSCs and adipose tissue explants stimulated with FGF1 followed by gene expression analyses of HMGA2 and p14Arf using Western-blot and qRT-PCR. Furthermore, canine subcutaneous white adipose tissue (WAT) were analyzed by qRT-PCR for their expression of HMGA2 and PPAR-gamma. Results: ADSCs and adipose tissue explants are able to execute a HMGA2 response upon FGF1 stimulation. FGF1 enhances proliferation of ADSCs by a HMGA2-dependent mechanism. In lipomas increase of HMGA2 is accompanied by increased expression of p14Arf. Furthermore, a significantly elevated level of HMGA2 in overweight dogs and a negative correlation between the expression of HMGA2 and PPAR-gamma in subcutaneous cWAT were noted. Conclusions: These results suggest that WAT contains cells that as essential part of adipogenesis up-regulate HMGA2 resulting from growth factor stimulation. In subgroups of lipoma, constitutive activation of HMGA2 due to rearrangements replaces the temporal response triggered by growth factors.},
  author       = {Thies, Helge W. and Nolte, Ingo and Wenk, Heiner and Mertens, Fredrik and Bullerdiek, Joern and Markowski, Dominique N.},
  issn         = {1930-739X},
  language     = {eng},
  number       = {1},
  pages        = {141--150},
  publisher    = {Nature Publishing Group},
  series       = {Obesity},
  title        = {Permanent activation of HMGA2 in lipomas mimics its temporal physiological activation linked to the gain of adipose tissue},
  url          = {http://dx.doi.org/10.1002/oby.20137},
  volume       = {22},
  year         = {2014},
}