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In vitro preparation of amelogenin nanoparticles carrying nucleic acids.

Svensson Bonde, Johan LU and Bülow, Leif LU (2014) In Biotechnology Letters 36(6). p.1349-1357
Abstract
Amelogenin, a matrix protein involved in biomineralization of enamel, can self-assemble to form nanospheres in a pH-dependent manner. Nucleic acids (single-stranded, double-stranded, and plasmid DNA, as well as RNA) could be co-precipitated with amelogenin, demonstrating a strong binding of nucleic acids to amelogenin. The amounts of co-precipitated nucleic acids were analyzed and binding levels upto 90 μg DNA/mg amelogenin was achieved. The co-precipitation could also be carried out in a bacterial cell homogenate, and no bacterial proteins were found in the amelogenin aggregates, suggesting specificity for nucleic acid binding. Dynamic light scattering showed that amelogenin nanosphere structure is maintained upon DNA binding with an upto... (More)
Amelogenin, a matrix protein involved in biomineralization of enamel, can self-assemble to form nanospheres in a pH-dependent manner. Nucleic acids (single-stranded, double-stranded, and plasmid DNA, as well as RNA) could be co-precipitated with amelogenin, demonstrating a strong binding of nucleic acids to amelogenin. The amounts of co-precipitated nucleic acids were analyzed and binding levels upto 90 μg DNA/mg amelogenin was achieved. The co-precipitation could also be carried out in a bacterial cell homogenate, and no bacterial proteins were found in the amelogenin aggregates, suggesting specificity for nucleic acid binding. Dynamic light scattering showed that amelogenin nanosphere structure is maintained upon DNA binding with an upto 2.6 nm increase in diameter. The reported binding of nucleic acids to amelogenin can be explored practically for nucleic acid separation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
36
issue
6
pages
1349 - 1357
publisher
Springer
external identifiers
  • pmid:24563322
  • wos:000335145800029
  • scopus:84899911653
ISSN
1573-6776
DOI
10.1007/s10529-014-1492-7
language
English
LU publication?
yes
id
dcce0355-3048-4212-9b22-5a750b5f0489 (old id 4334076)
date added to LUP
2014-03-06 12:43:44
date last changed
2017-01-01 03:56:59
@article{dcce0355-3048-4212-9b22-5a750b5f0489,
  abstract     = {Amelogenin, a matrix protein involved in biomineralization of enamel, can self-assemble to form nanospheres in a pH-dependent manner. Nucleic acids (single-stranded, double-stranded, and plasmid DNA, as well as RNA) could be co-precipitated with amelogenin, demonstrating a strong binding of nucleic acids to amelogenin. The amounts of co-precipitated nucleic acids were analyzed and binding levels upto 90 μg DNA/mg amelogenin was achieved. The co-precipitation could also be carried out in a bacterial cell homogenate, and no bacterial proteins were found in the amelogenin aggregates, suggesting specificity for nucleic acid binding. Dynamic light scattering showed that amelogenin nanosphere structure is maintained upon DNA binding with an upto 2.6 nm increase in diameter. The reported binding of nucleic acids to amelogenin can be explored practically for nucleic acid separation.},
  author       = {Svensson Bonde, Johan and Bülow, Leif},
  issn         = {1573-6776},
  language     = {eng},
  number       = {6},
  pages        = {1349--1357},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {In vitro preparation of amelogenin nanoparticles carrying nucleic acids.},
  url          = {http://dx.doi.org/10.1007/s10529-014-1492-7},
  volume       = {36},
  year         = {2014},
}