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Recombinant Antibody Microarray for Profiling the Serum Proteome of SLE.

Borrebaeck, Carl A K; Sturfelt, Gunnar LU and Wingren, Christer (2014) In Methods in Molecular Biology 1134. p.67-78
Abstract
Systemic lupus erythematosus (SLE) is a severe autoimmune connective tissue disease. Our current knowledge about the serum proteome, or serum biomarker panels, reflecting disease and disease status is still very limited. Affinity proteomics, represented by recombinant antibody arrays, is a novel, multiplex technology for high-throughput protein expression profiling of crude serum proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are deposited one by one in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified. The binding pattern is then converted into a relative protein expression map, or protein map, deciphering the... (More)
Systemic lupus erythematosus (SLE) is a severe autoimmune connective tissue disease. Our current knowledge about the serum proteome, or serum biomarker panels, reflecting disease and disease status is still very limited. Affinity proteomics, represented by recombinant antibody arrays, is a novel, multiplex technology for high-throughput protein expression profiling of crude serum proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are deposited one by one in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified. The binding pattern is then converted into a relative protein expression map, or protein map, deciphering the composition of the sample at the molecular level. The methodology provides unique opportunities for delineating serum biomarkers reflecting SLE, thus paving the way for improved diagnosis, classification, and prognosis. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1134
pages
67 - 78
publisher
Springer
external identifiers
  • pmid:24497355
  • scopus:84896301105
ISSN
1940-6029
DOI
10.1007/978-1-4939-0326-9_6
language
English
LU publication?
yes
id
e86231d8-7a49-44ee-a895-3474d9a34362 (old id 4335617)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24497355?dopt=Abstract
date added to LUP
2014-03-06 21:20:18
date last changed
2017-01-01 07:40:36
@article{e86231d8-7a49-44ee-a895-3474d9a34362,
  abstract     = {Systemic lupus erythematosus (SLE) is a severe autoimmune connective tissue disease. Our current knowledge about the serum proteome, or serum biomarker panels, reflecting disease and disease status is still very limited. Affinity proteomics, represented by recombinant antibody arrays, is a novel, multiplex technology for high-throughput protein expression profiling of crude serum proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are deposited one by one in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified. The binding pattern is then converted into a relative protein expression map, or protein map, deciphering the composition of the sample at the molecular level. The methodology provides unique opportunities for delineating serum biomarkers reflecting SLE, thus paving the way for improved diagnosis, classification, and prognosis.},
  author       = {Borrebaeck, Carl A K and Sturfelt, Gunnar and Wingren, Christer},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {67--78},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Recombinant Antibody Microarray for Profiling the Serum Proteome of SLE.},
  url          = {http://dx.doi.org/10.1007/978-1-4939-0326-9_6},
  volume       = {1134},
  year         = {2014},
}