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Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells.

Guloglu, Oktar LU ; Larsen, Anna and Brundin, Patrik LU (2014) In Journal of Neuroscience Research 92(5). p.564-573
Abstract
The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem... (More)
The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem cells with PA6-derived adipocytes reliably induces their differentiation into midbrain dopaminergic neurons. © 2014 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Neuroscience Research
volume
92
issue
5
pages
564 - 573
publisher
John Wiley & Sons
external identifiers
  • pmid:24482287
  • wos:000332553400003
  • scopus:84895929762
ISSN
1097-4547
DOI
10.1002/jnr.23355
language
English
LU publication?
yes
id
3c709665-47a7-4eca-a5d4-5007044db433 (old id 4336127)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24482287?dopt=Abstract
date added to LUP
2014-03-06 17:49:39
date last changed
2017-01-01 04:07:47
@article{3c709665-47a7-4eca-a5d4-5007044db433,
  abstract     = {The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem cells with PA6-derived adipocytes reliably induces their differentiation into midbrain dopaminergic neurons. © 2014 Wiley Periodicals, Inc.},
  author       = {Guloglu, Oktar and Larsen, Anna and Brundin, Patrik},
  issn         = {1097-4547},
  language     = {eng},
  number       = {5},
  pages        = {564--573},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells.},
  url          = {http://dx.doi.org/10.1002/jnr.23355},
  volume       = {92},
  year         = {2014},
}