Impact of immunization with Protein F on pulmonary clearance of nontypeable Haemophilus influenzae.
Jalalvand, Farshid LU ; Littorin, Nils LU ; Su, Shanice YC LU and Riesbeck, Kristian LU
(2014)
In Vaccine
32(20).
p.2261-2264
- Abstract
- Nontypeable Haemophilus influenzae (NTHi) is one of the main aetiologies of childhood bacterial infections as well as exacerbations in COPD patients. Currently, no licensed NTHi vaccine exists. In the present study, we evaluated the potential of the conserved and ubiquitous surface protein Haemophilus Protein F (PF) as a vaccine candidate. Our results show that incubation of NTHi with anti-PF antibodies significantly increased the opsonophygocytosis of human promyelocytic leukemia cell line-derived granulocytes, leading to efficient killing of the bacteria (P≤0.05). The presence of anti-PF IgG titers in healthy adults (n=60) was investigated, and we found that 26% of healthy blood donors carried antibodies with the main antigenic epitope... (More)
- Nontypeable Haemophilus influenzae (NTHi) is one of the main aetiologies of childhood bacterial infections as well as exacerbations in COPD patients. Currently, no licensed NTHi vaccine exists. In the present study, we evaluated the potential of the conserved and ubiquitous surface protein Haemophilus Protein F (PF) as a vaccine candidate. Our results show that incubation of NTHi with anti-PF antibodies significantly increased the opsonophygocytosis of human promyelocytic leukemia cell line-derived granulocytes, leading to efficient killing of the bacteria (P≤0.05). The presence of anti-PF IgG titers in healthy adults (n=60) was investigated, and we found that 26% of healthy blood donors carried antibodies with the main antigenic epitope being PF(23-48). Finally, mice immunized with PF(23-48) attained a significantly increased capacity to clear NTHi as compared to a control group immunized with a peptide derived from Moraxella catarrhalis β-lactamase (P≤0.05). Taken together, our results indicate that PF is a potential NTHi-vaccine candidate. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4383205
- author
- Jalalvand, Farshid
LU
; Littorin, Nils
LU
; Su, Shanice YC
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Vaccine
- volume
- 32
- issue
- 20
- pages
- 2261 - 2264
- publisher
- Elsevier
- external identifiers
-
- pmid:24631068
- wos:000335100600001
- pmid:24631068
- scopus:84897514225
- ISSN
- 1873-2518
- DOI
- 10.1016/j.vaccine.2014.02.082
- language
- English
- LU publication?
- yes
- id
- d0211980-068e-40d0-95ad-11fa8e9cfe78 (old id 4383205)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24631068?dopt=Abstract
- date added to LUP
- 2016-04-01 10:59:02
- date last changed
- 2022-02-02 22:52:16
@article{d0211980-068e-40d0-95ad-11fa8e9cfe78,
abstract = {{Nontypeable Haemophilus influenzae (NTHi) is one of the main aetiologies of childhood bacterial infections as well as exacerbations in COPD patients. Currently, no licensed NTHi vaccine exists. In the present study, we evaluated the potential of the conserved and ubiquitous surface protein Haemophilus Protein F (PF) as a vaccine candidate. Our results show that incubation of NTHi with anti-PF antibodies significantly increased the opsonophygocytosis of human promyelocytic leukemia cell line-derived granulocytes, leading to efficient killing of the bacteria (P≤0.05). The presence of anti-PF IgG titers in healthy adults (n=60) was investigated, and we found that 26% of healthy blood donors carried antibodies with the main antigenic epitope being PF(23-48). Finally, mice immunized with PF(23-48) attained a significantly increased capacity to clear NTHi as compared to a control group immunized with a peptide derived from Moraxella catarrhalis β-lactamase (P≤0.05). Taken together, our results indicate that PF is a potential NTHi-vaccine candidate.}},
author = {{Jalalvand, Farshid and Littorin, Nils and Su, Shanice YC and Riesbeck, Kristian}},
issn = {{1873-2518}},
language = {{eng}},
number = {{20}},
pages = {{2261--2264}},
publisher = {{Elsevier}},
series = {{Vaccine}},
title = {{Impact of immunization with Protein F on pulmonary clearance of nontypeable Haemophilus influenzae.}},
url = {{http://dx.doi.org/10.1016/j.vaccine.2014.02.082}},
doi = {{10.1016/j.vaccine.2014.02.082}},
volume = {{32}},
year = {{2014}},
}