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Impact of immunization with Protein F on pulmonary clearance of nontypeable Haemophilus influenzae.

Jalalvand, Farshid LU ; Littorin, Nils LU ; Su, Shanice YC LU and Riesbeck, Kristian LU (2014) In Vaccine 32(20). p.2261-2264
Abstract
Nontypeable Haemophilus influenzae (NTHi) is one of the main aetiologies of childhood bacterial infections as well as exacerbations in COPD patients. Currently, no licensed NTHi vaccine exists. In the present study, we evaluated the potential of the conserved and ubiquitous surface protein Haemophilus Protein F (PF) as a vaccine candidate. Our results show that incubation of NTHi with anti-PF antibodies significantly increased the opsonophygocytosis of human promyelocytic leukemia cell line-derived granulocytes, leading to efficient killing of the bacteria (P≤0.05). The presence of anti-PF IgG titers in healthy adults (n=60) was investigated, and we found that 26% of healthy blood donors carried antibodies with the main antigenic epitope... (More)
Nontypeable Haemophilus influenzae (NTHi) is one of the main aetiologies of childhood bacterial infections as well as exacerbations in COPD patients. Currently, no licensed NTHi vaccine exists. In the present study, we evaluated the potential of the conserved and ubiquitous surface protein Haemophilus Protein F (PF) as a vaccine candidate. Our results show that incubation of NTHi with anti-PF antibodies significantly increased the opsonophygocytosis of human promyelocytic leukemia cell line-derived granulocytes, leading to efficient killing of the bacteria (P≤0.05). The presence of anti-PF IgG titers in healthy adults (n=60) was investigated, and we found that 26% of healthy blood donors carried antibodies with the main antigenic epitope being PF(23-48). Finally, mice immunized with PF(23-48) attained a significantly increased capacity to clear NTHi as compared to a control group immunized with a peptide derived from Moraxella catarrhalis β-lactamase (P≤0.05). Taken together, our results indicate that PF is a potential NTHi-vaccine candidate. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vaccine
volume
32
issue
20
pages
2261 - 2264
publisher
Elsevier
external identifiers
  • pmid:24631068
  • wos:000335100600001
  • scopus:84897514225
ISSN
1873-2518
DOI
10.1016/j.vaccine.2014.02.082
language
English
LU publication?
yes
id
d0211980-068e-40d0-95ad-11fa8e9cfe78 (old id 4383205)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24631068?dopt=Abstract
date added to LUP
2014-04-03 16:36:32
date last changed
2017-05-28 03:22:31
@article{d0211980-068e-40d0-95ad-11fa8e9cfe78,
  abstract     = {Nontypeable Haemophilus influenzae (NTHi) is one of the main aetiologies of childhood bacterial infections as well as exacerbations in COPD patients. Currently, no licensed NTHi vaccine exists. In the present study, we evaluated the potential of the conserved and ubiquitous surface protein Haemophilus Protein F (PF) as a vaccine candidate. Our results show that incubation of NTHi with anti-PF antibodies significantly increased the opsonophygocytosis of human promyelocytic leukemia cell line-derived granulocytes, leading to efficient killing of the bacteria (P≤0.05). The presence of anti-PF IgG titers in healthy adults (n=60) was investigated, and we found that 26% of healthy blood donors carried antibodies with the main antigenic epitope being PF(23-48). Finally, mice immunized with PF(23-48) attained a significantly increased capacity to clear NTHi as compared to a control group immunized with a peptide derived from Moraxella catarrhalis β-lactamase (P≤0.05). Taken together, our results indicate that PF is a potential NTHi-vaccine candidate.},
  author       = {Jalalvand, Farshid and Littorin, Nils and Su, Shanice YC and Riesbeck, Kristian},
  issn         = {1873-2518},
  language     = {eng},
  number       = {20},
  pages        = {2261--2264},
  publisher    = {Elsevier},
  series       = {Vaccine},
  title        = {Impact of immunization with Protein F on pulmonary clearance of nontypeable Haemophilus influenzae.},
  url          = {http://dx.doi.org/10.1016/j.vaccine.2014.02.082},
  volume       = {32},
  year         = {2014},
}