Differential regulation of HIF-1α and HIF-2α in neuroblastoma: Estrogen-related receptor alpha (ERRα) regulates HIF2A transcription and correlates to poor outcome.
Hamidian, Arash LU ; von Stedingk, Kristoffer LU ; Thorén, Matilda LU ; Mohlin, Sofie LU
and Påhlman, Sven
LU
(2015)
In Biochemical and Biophysical Research Communications
461(3).
p.560-567
- Abstract
- Hypoxia-inducible factors (HIFs) are differentially regulated in tumor cells. While the current paradigm supports post-translational regulation of the HIF-α subunits, we recently showed that hypoxic HIF-2α is also transcriptionally regulated via insulin-like growth factor (IGF)-II in the childhood tumor neuroblastoma. Here, we demonstrate that transcriptional regulation of HIF-2α seems to be restricted to neural cell-derived tumors, while HIF-1α is canonically regulated at the post-translational level uniformly across different tumor forms. Enhanced expression of HIF2A mRNA at hypoxia is due to de novo transcription rather than increased mRNA stability, and chemical stabilization of the HIF-α proteins at oxygen-rich conditions unexpectedly... (More)
- Hypoxia-inducible factors (HIFs) are differentially regulated in tumor cells. While the current paradigm supports post-translational regulation of the HIF-α subunits, we recently showed that hypoxic HIF-2α is also transcriptionally regulated via insulin-like growth factor (IGF)-II in the childhood tumor neuroblastoma. Here, we demonstrate that transcriptional regulation of HIF-2α seems to be restricted to neural cell-derived tumors, while HIF-1α is canonically regulated at the post-translational level uniformly across different tumor forms. Enhanced expression of HIF2A mRNA at hypoxia is due to de novo transcription rather than increased mRNA stability, and chemical stabilization of the HIF-α proteins at oxygen-rich conditions unexpectedly leads to increased HIF2A transcription. The enhanced HIF2A levels do not seem to be dependent on active HIF-1. Using a transcriptome array approach, we identified members of the Peroxisome proliferator-activated receptor gamma coactivator (PGC)/Estrogen-related receptor (ERR) complex families as potential regulators of HIF2A. Knockdown or inhibition of one of the members, ERRα, leads to decreased expression of HIF2A, and high expression of the ERRα gene ESRRA correlates with poor overall and progression-free survival in a clinical neuroblastoma material consisting of 88 tumors. Thus, targeting of ERRα and pathways regulating transcriptional HIF-2α are promising therapeutic avenues in neuroblastoma. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5337855
- author
- Hamidian, Arash
LU
; von Stedingk, Kristoffer
LU
; Thorén, Matilda
LU
; Mohlin, Sofie
LU
and Påhlman, Sven
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- 461
- issue
- 3
- pages
- 560 - 567
- publisher
- Elsevier
- external identifiers
-
- pmid:25912138
- wos:000355157200021
- scopus:84937764558
- pmid:25912138
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2015.04.083
- language
- English
- LU publication?
- yes
- id
- 43d45027-a1d7-462e-9737-d2152a1ead00 (old id 5337855)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25912138?dopt=Abstract
- date added to LUP
- 2016-04-01 10:48:53
- date last changed
- 2022-02-02 21:17:43
@article{43d45027-a1d7-462e-9737-d2152a1ead00,
abstract = {{Hypoxia-inducible factors (HIFs) are differentially regulated in tumor cells. While the current paradigm supports post-translational regulation of the HIF-α subunits, we recently showed that hypoxic HIF-2α is also transcriptionally regulated via insulin-like growth factor (IGF)-II in the childhood tumor neuroblastoma. Here, we demonstrate that transcriptional regulation of HIF-2α seems to be restricted to neural cell-derived tumors, while HIF-1α is canonically regulated at the post-translational level uniformly across different tumor forms. Enhanced expression of HIF2A mRNA at hypoxia is due to de novo transcription rather than increased mRNA stability, and chemical stabilization of the HIF-α proteins at oxygen-rich conditions unexpectedly leads to increased HIF2A transcription. The enhanced HIF2A levels do not seem to be dependent on active HIF-1. Using a transcriptome array approach, we identified members of the Peroxisome proliferator-activated receptor gamma coactivator (PGC)/Estrogen-related receptor (ERR) complex families as potential regulators of HIF2A. Knockdown or inhibition of one of the members, ERRα, leads to decreased expression of HIF2A, and high expression of the ERRα gene ESRRA correlates with poor overall and progression-free survival in a clinical neuroblastoma material consisting of 88 tumors. Thus, targeting of ERRα and pathways regulating transcriptional HIF-2α are promising therapeutic avenues in neuroblastoma.}},
author = {{Hamidian, Arash and von Stedingk, Kristoffer and Thorén, Matilda and Mohlin, Sofie and Påhlman, Sven}},
issn = {{1090-2104}},
language = {{eng}},
number = {{3}},
pages = {{560--567}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Differential regulation of HIF-1α and HIF-2α in neuroblastoma: Estrogen-related receptor alpha (ERRα) regulates HIF2A transcription and correlates to poor outcome.}},
url = {{https://lup.lub.lu.se/search/files/2156546/8147126}},
doi = {{10.1016/j.bbrc.2015.04.083}},
volume = {{461}},
year = {{2015}},
}