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Microarray Analysis Reveals Moderate Gene Expression Changes in Cortical Neural Stem Cells Cultured on Nanowire Arrays

SanMartin, Agneta LU ; Johansson, Fredrik LU ; Samuelson, Lars LU and Prinz, Christelle LU (2014) In Journal of Nanoscience and Nanotechnology 14(7). p.4880-4885
Abstract
Although nanowires are widely used in biological applications, especially as cell-manipulation tools, the effect of nanowires on living cells has not been fully investigated. Here, we examined the full gene expression of mouse cortical stem cells cultured on vertical nanowire substrates using RNA microarrays. Genes involved in cell adhesion, cell morphology regulation and cell metabolism were up regulated on the nanowire substrates as compared to flat controls. Scanning electron microscopy images of the samples corroborated our findings, revealing significant differences in cell morphology and adhesion between the nanowire and the flat substrates. Cells on nanowires exhibited a round morphology with a mature appearance characterized by... (More)
Although nanowires are widely used in biological applications, especially as cell-manipulation tools, the effect of nanowires on living cells has not been fully investigated. Here, we examined the full gene expression of mouse cortical stem cells cultured on vertical nanowire substrates using RNA microarrays. Genes involved in cell adhesion, cell morphology regulation and cell metabolism were up regulated on the nanowire substrates as compared to flat controls. Scanning electron microscopy images of the samples corroborated our findings, revealing significant differences in cell morphology and adhesion between the nanowire and the flat substrates. Cells on nanowires exhibited a round morphology with a mature appearance characterized by numerous and long processes adhering to the nanowires, while cells on flat substrates had a flat, spread-out morphology and very little neurite outgrowth. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gene Expression, Nanowires, Stem Cells, Cellular Stress, Cell, Morphology, Microarray
in
Journal of Nanoscience and Nanotechnology
volume
14
issue
7
pages
4880 - 4885
publisher
American Scientific Publishers
external identifiers
  • wos:000332926400016
  • scopus:84901633074
ISSN
1533-4880
DOI
10.1166/jnn.2014.8669
language
English
LU publication?
yes
id
1afeaae2-6908-41c6-83d0-986a0dcd59f4 (old id 4407443)
date added to LUP
2014-04-28 14:43:27
date last changed
2017-07-23 03:06:09
@article{1afeaae2-6908-41c6-83d0-986a0dcd59f4,
  abstract     = {Although nanowires are widely used in biological applications, especially as cell-manipulation tools, the effect of nanowires on living cells has not been fully investigated. Here, we examined the full gene expression of mouse cortical stem cells cultured on vertical nanowire substrates using RNA microarrays. Genes involved in cell adhesion, cell morphology regulation and cell metabolism were up regulated on the nanowire substrates as compared to flat controls. Scanning electron microscopy images of the samples corroborated our findings, revealing significant differences in cell morphology and adhesion between the nanowire and the flat substrates. Cells on nanowires exhibited a round morphology with a mature appearance characterized by numerous and long processes adhering to the nanowires, while cells on flat substrates had a flat, spread-out morphology and very little neurite outgrowth.},
  author       = {SanMartin, Agneta and Johansson, Fredrik and Samuelson, Lars and Prinz, Christelle},
  issn         = {1533-4880},
  keyword      = {Gene Expression,Nanowires,Stem Cells,Cellular Stress,Cell,Morphology,Microarray},
  language     = {eng},
  number       = {7},
  pages        = {4880--4885},
  publisher    = {American Scientific Publishers},
  series       = {Journal of Nanoscience and Nanotechnology},
  title        = {Microarray Analysis Reveals Moderate Gene Expression Changes in Cortical Neural Stem Cells Cultured on Nanowire Arrays},
  url          = {http://dx.doi.org/10.1166/jnn.2014.8669},
  volume       = {14},
  year         = {2014},
}