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Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis.

Petersson, Linn; Dexlin-Mellby, Linda; Bengtsson, Anders LU ; Sturfelt, Gunnar LU ; Borrebaeck, Carl A K and Wingren, Christer (2014) In Lab on A Chip 14(11). p.1931-1942
Abstract
In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first... (More)
In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first clinical application targeting SLE nephritis, a severe chronic autoimmune connective tissue disorder, as the model disease. To this end, crude, directly biotinylated serum samples were targeted. The results showed that the miniaturized and multiplexed array platform displayed adequate performance, and that SLE-associated serum biomarker panels reflecting the disease process could be deciphered, outlining the use of miniaturized antibody arrays for disease proteomics and biomarker discovery. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
14
issue
11
pages
1931 - 1942
publisher
Royal Society of Chemistry
external identifiers
  • wos:000335925400019
  • pmid:24763547
  • scopus:84899873765
ISSN
1473-0189
DOI
10.1039/c3lc51420j
language
English
LU publication?
yes
id
cd455539-64fe-47cb-a31c-e70d4fb289cd (old id 4429505)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24763547?dopt=Abstract
date added to LUP
2014-05-03 22:28:53
date last changed
2017-10-08 03:12:24
@article{cd455539-64fe-47cb-a31c-e70d4fb289cd,
  abstract     = {In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first clinical application targeting SLE nephritis, a severe chronic autoimmune connective tissue disorder, as the model disease. To this end, crude, directly biotinylated serum samples were targeted. The results showed that the miniaturized and multiplexed array platform displayed adequate performance, and that SLE-associated serum biomarker panels reflecting the disease process could be deciphered, outlining the use of miniaturized antibody arrays for disease proteomics and biomarker discovery.},
  author       = {Petersson, Linn and Dexlin-Mellby, Linda and Bengtsson, Anders and Sturfelt, Gunnar and Borrebaeck, Carl A K and Wingren, Christer},
  issn         = {1473-0189},
  language     = {eng},
  number       = {11},
  pages        = {1931--1942},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis.},
  url          = {http://dx.doi.org/10.1039/c3lc51420j},
  volume       = {14},
  year         = {2014},
}