Acoustic actuated fluorescence activated sorting of microparticles.
(2014) In Lab on a Chip 14(11). p.1943-1950- Abstract
- In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a... (More)
- In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4429514
- author
- Jakobsson, Ola LU ; Grenvall, Carl LU ; Nordin, Maria LU ; Evander, Mikael LU and Laurell, Thomas LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 14
- issue
- 11
- pages
- 1943 - 1950
- publisher
- Royal Society of Chemistry
- external identifiers
-
- pmid:24763517
- wos:000335925400020
- scopus:84899824858
- pmid:24763517
- ISSN
- 1473-0189
- DOI
- 10.1039/c3lc51408k
- language
- English
- LU publication?
- yes
- id
- 1171b335-8c35-49bd-96dc-b3593d11f48f (old id 4429514)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24763517?dopt=Abstract
- date added to LUP
- 2016-04-01 10:34:10
- date last changed
- 2022-04-27 23:20:52
@article{1171b335-8c35-49bd-96dc-b3593d11f48f,
abstract = {{In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications.}},
author = {{Jakobsson, Ola and Grenvall, Carl and Nordin, Maria and Evander, Mikael and Laurell, Thomas}},
issn = {{1473-0189}},
language = {{eng}},
number = {{11}},
pages = {{1943--1950}},
publisher = {{Royal Society of Chemistry}},
series = {{Lab on a Chip}},
title = {{Acoustic actuated fluorescence activated sorting of microparticles.}},
url = {{http://dx.doi.org/10.1039/c3lc51408k}},
doi = {{10.1039/c3lc51408k}},
volume = {{14}},
year = {{2014}},
}