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Acoustic actuated fluorescence activated sorting of microparticles.

Jakobsson, Ola LU ; Grenvall, Carl LU ; Nordin, Maria LU ; Evander, Mikael LU and Laurell, Thomas LU (2014) In Lab on A Chip 14(11). p.1943-1950
Abstract
In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a... (More)
In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
14
issue
11
pages
1943 - 1950
publisher
Royal Society of Chemistry
external identifiers
  • pmid:24763517
  • wos:000335925400020
  • scopus:84899824858
ISSN
1473-0189
DOI
10.1039/c3lc51408k
language
English
LU publication?
yes
id
1171b335-8c35-49bd-96dc-b3593d11f48f (old id 4429514)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24763517?dopt=Abstract
date added to LUP
2014-05-03 22:30:59
date last changed
2017-10-01 03:18:21
@article{1171b335-8c35-49bd-96dc-b3593d11f48f,
  abstract     = {In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications.},
  author       = {Jakobsson, Ola and Grenvall, Carl and Nordin, Maria and Evander, Mikael and Laurell, Thomas},
  issn         = {1473-0189},
  language     = {eng},
  number       = {11},
  pages        = {1943--1950},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Acoustic actuated fluorescence activated sorting of microparticles.},
  url          = {http://dx.doi.org/10.1039/c3lc51408k},
  volume       = {14},
  year         = {2014},
}