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Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions

Stella, Roberto; Arrigoni, Giorgio; Biancotto, Giancarlo; Krogh, Morten LU ; Vascellari, Marta; Lega, Francesca; Pozza, Giandomenico; Angeletti, Roberto; Andrighetto, Igino and James, Peter LU (2014) In Journal of Proteome Research 13(4). p.1794-1799
Abstract
In veal calf production, growth promoters are still illicitly used. Surveillance of misuse of such molecules is necessary to preserve human health. Methods currently adopted for their analysis are based on liquid chromatography tandem mass spectrometry, but their efficacy can be affected by undetectable residual concentrations in biological matrices due to treatments at low-dosage or based on unknown anabolic compounds. The development of screening methods to identify the indirect biological effects of administration of growth promoters can improve the efficiency of drug residue monitoring. To this purpose, an integrated approach has been used to further validate the set of protein biomarkers defined in a previous controlled study to... (More)
In veal calf production, growth promoters are still illicitly used. Surveillance of misuse of such molecules is necessary to preserve human health. Methods currently adopted for their analysis are based on liquid chromatography tandem mass spectrometry, but their efficacy can be affected by undetectable residual concentrations in biological matrices due to treatments at low-dosage or based on unknown anabolic compounds. The development of screening methods to identify the indirect biological effects of administration of growth promoters can improve the efficiency of drug residue monitoring. To this purpose, an integrated approach has been used to further validate the set of protein biomarkers defined in a previous controlled study to detect the use of corticosteroids through the changes caused in muscle protein expression. The thymus morphology of 48 samples collected under field conditions was evaluated to assess the presence of potential corticosteroids treatment. Animals were divided on the basis of their thymus characteristics in negative or suspected for illegal corticosteroids treatment. Drug residue analyses were performed on the liver, giving a satisfactory correlation with the histological examination (similar to 85%). Finally, the proteomics analysis of muscle protein extracts was carried out by 2D differential in gel electrophoresis, and proteins that were differentially expressed between the two animal groups (p value <0.01) were selected for MALDI-MS/MS analysis. This approach allowed us to identify 29 different proteins, and our findings indicate that the altered protein expression pattern can be used as an indirect method for the detection of illicit corticosteroids administration. A subset of the identified proteins was already reported in a previous controlled study, proving that these biomarkers can be used to develop a screening assay to improve the tools currently available for the detection of corticosteroids abuse in bovine meat production. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
corticosteroids, veal calves, indirect markers, growth promoters, proteomics
in
Journal of Proteome Research
volume
13
issue
4
pages
1794 - 1799
publisher
The American Chemical Society
external identifiers
  • wos:000334016400002
  • scopus:84898735674
ISSN
1535-3893
DOI
10.1021/pr401193r
language
English
LU publication?
yes
id
4f6c32a1-b9d1-4f7b-aee6-ae95f3e18714 (old id 4439612)
date added to LUP
2014-05-20 11:24:10
date last changed
2017-01-01 04:16:50
@article{4f6c32a1-b9d1-4f7b-aee6-ae95f3e18714,
  abstract     = {In veal calf production, growth promoters are still illicitly used. Surveillance of misuse of such molecules is necessary to preserve human health. Methods currently adopted for their analysis are based on liquid chromatography tandem mass spectrometry, but their efficacy can be affected by undetectable residual concentrations in biological matrices due to treatments at low-dosage or based on unknown anabolic compounds. The development of screening methods to identify the indirect biological effects of administration of growth promoters can improve the efficiency of drug residue monitoring. To this purpose, an integrated approach has been used to further validate the set of protein biomarkers defined in a previous controlled study to detect the use of corticosteroids through the changes caused in muscle protein expression. The thymus morphology of 48 samples collected under field conditions was evaluated to assess the presence of potential corticosteroids treatment. Animals were divided on the basis of their thymus characteristics in negative or suspected for illegal corticosteroids treatment. Drug residue analyses were performed on the liver, giving a satisfactory correlation with the histological examination (similar to 85%). Finally, the proteomics analysis of muscle protein extracts was carried out by 2D differential in gel electrophoresis, and proteins that were differentially expressed between the two animal groups (p value &lt;0.01) were selected for MALDI-MS/MS analysis. This approach allowed us to identify 29 different proteins, and our findings indicate that the altered protein expression pattern can be used as an indirect method for the detection of illicit corticosteroids administration. A subset of the identified proteins was already reported in a previous controlled study, proving that these biomarkers can be used to develop a screening assay to improve the tools currently available for the detection of corticosteroids abuse in bovine meat production.},
  author       = {Stella, Roberto and Arrigoni, Giorgio and Biancotto, Giancarlo and Krogh, Morten and Vascellari, Marta and Lega, Francesca and Pozza, Giandomenico and Angeletti, Roberto and Andrighetto, Igino and James, Peter},
  issn         = {1535-3893},
  keyword      = {corticosteroids,veal calves,indirect markers,growth promoters,proteomics},
  language     = {eng},
  number       = {4},
  pages        = {1794--1799},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions},
  url          = {http://dx.doi.org/10.1021/pr401193r},
  volume       = {13},
  year         = {2014},
}