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Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Xie, Haiyan LU ; Xie, Zhiyuan; Mousavi, Monirehalsadat LU ; Bendsöe, Niels LU ; Brydegaard, Mikkel LU ; Axelsson, Johan LU and Andersson-Engels, Stefan LU (2014) In Journal of Biomedical Optics 19(7). p.11-071408
Abstract (Swedish)
Abstract in Undetermined

ABSTRACT. Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue... (More)
Abstract in Undetermined

ABSTRACT. Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue optical properties, and ambient light suppression. Laboratory measurements show the system is insensitive to strong ambient light. Validation measurements of tissue phantoms using nonlinear least squares support vector machines (LS-SVM) regression analysis demonstrate an error of <5% for PpIX concentration ranging from 400 to 1000 nM, even in the presence of large variations in phantom optical properties. The mean error is 3% for reduced scattering coefficient and 5% for blood concentration. Diagnostic precision of 100% was obtained by LS-SVM classification for in vivo skin tumors with topically applied 5-aminolevulinic acid during photodynamic therapy. The probe could easily be generalized to other tissue types and fluorophores for therapy guidance and monitoring. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fluorescence spectroscopy, fiber optic sensors, reflectance, tissue, light-emitting diodes
in
Journal of Biomedical Optics
volume
19
issue
7
pages
11 - 071408
publisher
Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
external identifiers
  • pmid:24623193
  • wos:000340490400013
  • scopus:84896354280
ISSN
1083-3668
DOI
10.1117/1.JBO.19.7.071408
language
English
LU publication?
yes
id
025e6923-08c4-49a9-82d2-14aed67eb41f (old id 4446699)
alternative location
http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1849759
date added to LUP
2014-05-20 23:18:18
date last changed
2017-01-01 03:08:55
@article{025e6923-08c4-49a9-82d2-14aed67eb41f,
  abstract     = {<b>Abstract in Undetermined</b><br/><br>
ABSTRACT. Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue optical properties, and ambient light suppression. Laboratory measurements show the system is insensitive to strong ambient light. Validation measurements of tissue phantoms using nonlinear least squares support vector machines (LS-SVM) regression analysis demonstrate an error of &lt;5% for PpIX concentration ranging from 400 to 1000 nM, even in the presence of large variations in phantom optical properties. The mean error is 3% for reduced scattering coefficient and 5% for blood concentration. Diagnostic precision of 100% was obtained by LS-SVM classification for in vivo skin tumors with topically applied 5-aminolevulinic acid during photodynamic therapy. The probe could easily be generalized to other tissue types and fluorophores for therapy guidance and monitoring.},
  author       = {Xie, Haiyan and Xie, Zhiyuan and Mousavi, Monirehalsadat and Bendsöe, Niels and Brydegaard, Mikkel and Axelsson, Johan and Andersson-Engels, Stefan},
  issn         = {1083-3668},
  keyword      = {fluorescence spectroscopy,fiber optic sensors,reflectance,tissue,light-emitting diodes},
  language     = {eng},
  number       = {7},
  pages        = {11--071408},
  publisher    = {Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society},
  series       = {Journal of Biomedical Optics},
  title        = {Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring},
  url          = {http://dx.doi.org/10.1117/1.JBO.19.7.071408},
  volume       = {19},
  year         = {2014},
}