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Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach

Bengtsson, Therese; Weighill, Deborah; Proux-Wera, Estelle; Levander, Fredrik LU ; Resjo, Svante; Burra, Dharani Dhar; Moushib, Laith LU ; Hedley, Pete E.; Liljeroth, Erland and Jacobson, Dan, et al. (2014) In BMC Genomics 15.
Abstract
Background: Induced resistance (IR) can be part of a sustainable plant protection strategy against important plant diseases. beta-aminobutyric acid (BABA) can induce resistance in a wide range of plants against several types of pathogens, including potato infected with Phytophthora infestans. However, the molecular mechanisms behind this are unclear and seem to be dependent on the system studied. To elucidate the defence responses activated by BABA in potato, a genome-wide transcript microarray analysis in combination with label-free quantitative proteomics analysis of the apoplast secretome were performed two days after treatment of the leaf canopy with BABA at two concentrations, 1 and 10 mM. Results: Over 5000 transcripts were... (More)
Background: Induced resistance (IR) can be part of a sustainable plant protection strategy against important plant diseases. beta-aminobutyric acid (BABA) can induce resistance in a wide range of plants against several types of pathogens, including potato infected with Phytophthora infestans. However, the molecular mechanisms behind this are unclear and seem to be dependent on the system studied. To elucidate the defence responses activated by BABA in potato, a genome-wide transcript microarray analysis in combination with label-free quantitative proteomics analysis of the apoplast secretome were performed two days after treatment of the leaf canopy with BABA at two concentrations, 1 and 10 mM. Results: Over 5000 transcripts were differentially expressed and over 90 secretome proteins changed in abundance indicating a massive activation of defence mechanisms with 10 mM BABA, the concentration effective against late blight disease. To aid analysis, we present a more comprehensive functional annotation of the microarray probes and gene models by retrieving information from orthologous gene families across 26 sequenced plant genomes. The new annotation provided GO terms to 8616 previously un-annotated probes. Conclusions: BABA at 10 mM affected several processes related to plant hormones and amino acid metabolism. A major accumulation of PR proteins was also evident, and in the mevalonate pathway, genes involved in sterol biosynthesis were down-regulated, whereas several enzymes involved in the sesquiterpene phytoalexin biosynthesis were up-regulated. Interestingly, abscisic acid (ABA) responsive genes were not as clearly regulated by BABA in potato as previously reported in Arabidopsis. Together these findings provide candidates and markers for improved resistance in potato, one of the most important crops in the world. (Less)
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keywords
Functional annotation, Mevalonate pathway, Phytophthora infestans, Secretome, Solanum tuberosum, Sterol biosynthesis
in
BMC Genomics
volume
15
publisher
BioMed Central
external identifiers
  • wos:000335409900001
  • scopus:84899967180
ISSN
1471-2164
DOI
10.1186/1471-2164-15-315
language
English
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yes
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35fb1d7a-e1e5-4d65-9370-906539749a14 (old id 4482575)
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2014-06-19 14:40:56
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2017-10-01 04:14:43
@article{35fb1d7a-e1e5-4d65-9370-906539749a14,
  abstract     = {Background: Induced resistance (IR) can be part of a sustainable plant protection strategy against important plant diseases. beta-aminobutyric acid (BABA) can induce resistance in a wide range of plants against several types of pathogens, including potato infected with Phytophthora infestans. However, the molecular mechanisms behind this are unclear and seem to be dependent on the system studied. To elucidate the defence responses activated by BABA in potato, a genome-wide transcript microarray analysis in combination with label-free quantitative proteomics analysis of the apoplast secretome were performed two days after treatment of the leaf canopy with BABA at two concentrations, 1 and 10 mM. Results: Over 5000 transcripts were differentially expressed and over 90 secretome proteins changed in abundance indicating a massive activation of defence mechanisms with 10 mM BABA, the concentration effective against late blight disease. To aid analysis, we present a more comprehensive functional annotation of the microarray probes and gene models by retrieving information from orthologous gene families across 26 sequenced plant genomes. The new annotation provided GO terms to 8616 previously un-annotated probes. Conclusions: BABA at 10 mM affected several processes related to plant hormones and amino acid metabolism. A major accumulation of PR proteins was also evident, and in the mevalonate pathway, genes involved in sterol biosynthesis were down-regulated, whereas several enzymes involved in the sesquiterpene phytoalexin biosynthesis were up-regulated. Interestingly, abscisic acid (ABA) responsive genes were not as clearly regulated by BABA in potato as previously reported in Arabidopsis. Together these findings provide candidates and markers for improved resistance in potato, one of the most important crops in the world.},
  author       = {Bengtsson, Therese and Weighill, Deborah and Proux-Wera, Estelle and Levander, Fredrik and Resjo, Svante and Burra, Dharani Dhar and Moushib, Laith and Hedley, Pete E. and Liljeroth, Erland and Jacobson, Dan and Alexandersson, Erik and Andreasson, Erik},
  issn         = {1471-2164},
  keyword      = {Functional annotation,Mevalonate pathway,Phytophthora infestans,Secretome,Solanum tuberosum,Sterol biosynthesis},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Genomics},
  title        = {Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach},
  url          = {http://dx.doi.org/10.1186/1471-2164-15-315},
  volume       = {15},
  year         = {2014},
}