Selective Generation of Gut Tropic T Cells in Gut-associated Lymphoid Tissue (GALT): Requirement for GALT Dendritic Cells and Adjuvant.
(2003) In Journal of Experimental Medicine 198(6). p.963-969- Abstract
- n the current study, we address the underlying mechanism for the selective generation of gut-homing T cells in the gut-associated lymphoid tissues (GALT). We demonstrate that DCs in the GALT are unique in their capacity to establish T cell gut tropism but in vivo only confer this property to T cells in the presence of DC maturational stimuli, including toll-like receptor-dependent and -independent adjuvants. Thus, DCs from mesenteric LNs (MLNs), but not from spleen, supported expression of the chemokine receptor CCR9 and integrin {alpha}4ß7 by activated CD8+ T cells. While DCs were also required for an efficient down-regulation of CD62L, this function was not restricted to MLN DCs. In an adoptive CD8+ T cell transfer model,... (More)
- n the current study, we address the underlying mechanism for the selective generation of gut-homing T cells in the gut-associated lymphoid tissues (GALT). We demonstrate that DCs in the GALT are unique in their capacity to establish T cell gut tropism but in vivo only confer this property to T cells in the presence of DC maturational stimuli, including toll-like receptor-dependent and -independent adjuvants. Thus, DCs from mesenteric LNs (MLNs), but not from spleen, supported expression of the chemokine receptor CCR9 and integrin {alpha}4ß7 by activated CD8+ T cells. While DCs were also required for an efficient down-regulation of CD62L, this function was not restricted to MLN DCs. In an adoptive CD8+ T cell transfer model, antigen-specific T cells entering the small intestinal epithelium were homogeneously CCR9+{alpha}4ß7+CD62Llow, and this phenotype was only generated in GALT and in the presence of adjuvant. Consistent with the CCR9+ phenotype of the gut-homing T cells, CCR9 was found to play a critical role in the localization of T cells to the small intestinal epithelium. Together, these results demonstrate that GALT DCs and T cell expression of CCR9 play critical and integrated roles during T cell homing to the gut. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/117884
- author
- Johansson Lindbom, Bengt LU ; Svensson Frej, Marcus LU ; Wurbel, Marc-Andre ; Malissen, Bernard ; Marquez, Gabriel and Agace, William LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- lymphocytes, antigen-presenting cell, inflammation, chemokines, intestinal mucosa
- in
- Journal of Experimental Medicine
- volume
- 198
- issue
- 6
- pages
- 963 - 969
- publisher
- Rockefeller University Press
- external identifiers
-
- wos:000185416200013
- pmid:12963696
- scopus:0141449950
- ISSN
- 1540-9538
- DOI
- 10.1084/jem.20031244
- language
- English
- LU publication?
- yes
- id
- 44c0ee98-387b-4b99-be4f-2bd37f3ff837 (old id 117884)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12963696&dopt=Abstract
- date added to LUP
- 2016-04-01 15:38:43
- date last changed
- 2022-04-22 08:41:42
@article{44c0ee98-387b-4b99-be4f-2bd37f3ff837, abstract = {{n the current study, we address the underlying mechanism for the selective generation of gut-homing T cells in the gut-associated lymphoid tissues (GALT). We demonstrate that DCs in the GALT are unique in their capacity to establish T cell gut tropism but in vivo only confer this property to T cells in the presence of DC maturational stimuli, including toll-like receptor-dependent and -independent adjuvants. Thus, DCs from mesenteric LNs (MLNs), but not from spleen, supported expression of the chemokine receptor CCR9 and integrin {alpha}4ß7 by activated CD8+ T cells. While DCs were also required for an efficient down-regulation of CD62L, this function was not restricted to MLN DCs. In an adoptive CD8+ T cell transfer model, antigen-specific T cells entering the small intestinal epithelium were homogeneously CCR9+{alpha}4ß7+CD62Llow, and this phenotype was only generated in GALT and in the presence of adjuvant. Consistent with the CCR9+ phenotype of the gut-homing T cells, CCR9 was found to play a critical role in the localization of T cells to the small intestinal epithelium. Together, these results demonstrate that GALT DCs and T cell expression of CCR9 play critical and integrated roles during T cell homing to the gut.}}, author = {{Johansson Lindbom, Bengt and Svensson Frej, Marcus and Wurbel, Marc-Andre and Malissen, Bernard and Marquez, Gabriel and Agace, William}}, issn = {{1540-9538}}, keywords = {{lymphocytes; antigen-presenting cell; inflammation; chemokines; intestinal mucosa}}, language = {{eng}}, number = {{6}}, pages = {{963--969}}, publisher = {{Rockefeller University Press}}, series = {{Journal of Experimental Medicine}}, title = {{Selective Generation of Gut Tropic T Cells in Gut-associated Lymphoid Tissue (GALT): Requirement for GALT Dendritic Cells and Adjuvant.}}, url = {{https://lup.lub.lu.se/search/files/4439696/623867.pdf}}, doi = {{10.1084/jem.20031244}}, volume = {{198}}, year = {{2003}}, }