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Minimal and hybrid hydrogenases are active from archaea

Greening, Chris ; Cabotaje, Princess R ; Valentin Alvarado, Luis E ; Leung, Pok Man ; Land, Henrik ; Rodrigues-Oliveira, Thiago ; Ponce-Toledo, Rafael I ; Senger, Moritz ; Klamke, Max A and Milton, Michael , et al. (2024) In Cell 187.
Abstract

Microbial hydrogen (H
2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H
2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H
2. Moreover, we identify and characterize... (More)

Microbial hydrogen (H
2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H
2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H
2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H
2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H
2-metabolizing enzymes.

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organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Cell
volume
187
publisher
Cell Press
external identifiers
  • pmid:38866018
ISSN
1097-4172
DOI
10.1016/j.cell.2024.05.032
language
English
LU publication?
yes
additional info
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.
id
44d20f73-2549-4f7c-a17a-f1c4cc1c021a
date added to LUP
2024-06-17 09:09:00
date last changed
2024-06-17 17:09:05
@article{44d20f73-2549-4f7c-a17a-f1c4cc1c021a,
  abstract     = {{<p>Microbial hydrogen (H<br>
 2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H<br>
 2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H<br>
 2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H <br>
 2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H<br>
 2-metabolizing enzymes.<br>
 </p>}},
  author       = {{Greening, Chris and Cabotaje, Princess R and Valentin Alvarado, Luis E and Leung, Pok Man and Land, Henrik and Rodrigues-Oliveira, Thiago and Ponce-Toledo, Rafael I and Senger, Moritz and Klamke, Max A and Milton, Michael and Lappan, Rachael and Mullen, Susan and West-Roberts, Jacob and Mao, Jie and Song, Jiangning and Schoelmerich, Marie and Stairs, Courtney W and Schleper, Christa and Grinter, Rhys and Spang, Anja and Banfield, Jillian F and Berggren, Gustav}},
  issn         = {{1097-4172}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{Cell Press}},
  series       = {{Cell}},
  title        = {{Minimal and hybrid hydrogenases are active from archaea}},
  url          = {{http://dx.doi.org/10.1016/j.cell.2024.05.032}},
  doi          = {{10.1016/j.cell.2024.05.032}},
  volume       = {{187}},
  year         = {{2024}},
}