Minimal and hybrid hydrogenases are active from archaea
(2024) In Cell 187.- Abstract
Microbial hydrogen (H
2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H
2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H
2. Moreover, we identify and characterize... (More)Microbial hydrogen (H
(Less)
2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H
2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H
2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H
2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H
2-metabolizing enzymes.
- author
- organization
- publishing date
- 2024-06-07
- type
- Contribution to journal
- publication status
- epub
- subject
- in
- Cell
- volume
- 187
- publisher
- Cell Press
- external identifiers
-
- pmid:38866018
- ISSN
- 1097-4172
- DOI
- 10.1016/j.cell.2024.05.032
- language
- English
- LU publication?
- yes
- additional info
- Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.
- id
- 44d20f73-2549-4f7c-a17a-f1c4cc1c021a
- date added to LUP
- 2024-06-17 09:09:00
- date last changed
- 2024-06-17 17:09:05
@article{44d20f73-2549-4f7c-a17a-f1c4cc1c021a, abstract = {{<p>Microbial hydrogen (H<br> 2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H<br> 2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H<br> 2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H <br> 2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H<br> 2-metabolizing enzymes.<br> </p>}}, author = {{Greening, Chris and Cabotaje, Princess R and Valentin Alvarado, Luis E and Leung, Pok Man and Land, Henrik and Rodrigues-Oliveira, Thiago and Ponce-Toledo, Rafael I and Senger, Moritz and Klamke, Max A and Milton, Michael and Lappan, Rachael and Mullen, Susan and West-Roberts, Jacob and Mao, Jie and Song, Jiangning and Schoelmerich, Marie and Stairs, Courtney W and Schleper, Christa and Grinter, Rhys and Spang, Anja and Banfield, Jillian F and Berggren, Gustav}}, issn = {{1097-4172}}, language = {{eng}}, month = {{06}}, publisher = {{Cell Press}}, series = {{Cell}}, title = {{Minimal and hybrid hydrogenases are active from archaea}}, url = {{http://dx.doi.org/10.1016/j.cell.2024.05.032}}, doi = {{10.1016/j.cell.2024.05.032}}, volume = {{187}}, year = {{2024}}, }