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Miniaturization of multiplexed planar recombinant antibody arrays for serum protein profiling.

Petersson, Linn; Coen, Michael; Amro, Nabil A; Truedsson, Lennart LU ; Borrebaeck, Carl Ak and Wingren, Christer (2014) In Bioanalysis 6(9). p.1175-1185
Abstract
Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen... (More)
Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen nanolithography technology, and interfaced with a high-resolution fluorescent-based scanner. The reproducibility, sensitivity, specificity, and applicability of the set-up were demonstrated by profiling a set of well-characterized serum samples. Conclusion: The designed antibody array platform opens up new possibilities for large-scale, multiplex profiling of crude proteomes in a miniaturized fashion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioanalysis
volume
6
issue
9
pages
1175 - 1185
publisher
Future Science
external identifiers
  • pmid:24946919
  • wos:000337945200012
  • scopus:84903121058
ISSN
1757-6180
DOI
10.4155/bio.13.342
language
English
LU publication?
yes
id
bc3f96e5-1b27-4814-84f0-e441e4f23691 (old id 4527947)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24946919?dopt=Abstract
date added to LUP
2014-07-06 19:12:55
date last changed
2017-01-01 03:10:57
@article{bc3f96e5-1b27-4814-84f0-e441e4f23691,
  abstract     = {Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm(2)/spot) and the array density was increased 19-times (38,000 spots/cm(2)). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen nanolithography technology, and interfaced with a high-resolution fluorescent-based scanner. The reproducibility, sensitivity, specificity, and applicability of the set-up were demonstrated by profiling a set of well-characterized serum samples. Conclusion: The designed antibody array platform opens up new possibilities for large-scale, multiplex profiling of crude proteomes in a miniaturized fashion.},
  author       = {Petersson, Linn and Coen, Michael and Amro, Nabil A and Truedsson, Lennart and Borrebaeck, Carl Ak and Wingren, Christer},
  issn         = {1757-6180},
  language     = {eng},
  number       = {9},
  pages        = {1175--1185},
  publisher    = {Future Science},
  series       = {Bioanalysis},
  title        = {Miniaturization of multiplexed planar recombinant antibody arrays for serum protein profiling.},
  url          = {http://dx.doi.org/10.4155/bio.13.342},
  volume       = {6},
  year         = {2014},
}