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Interaction of fengycin with stratum corneum mimicking model membranes: A calorimetry study.

Eeman, Marc; Olofsson, Gerd LU ; Sparr, Emma LU ; Nasir, Mehmet Nail; Nylander, Tommy LU and Deleu, Magali (2014) In Colloids and Surfaces B: Biointerfaces 121. p.27-35
Abstract
Based on its outstanding antifungal properties, it is reasonable to believe that fengycin might be efficient to topically treat localized dermatomycoses. Since most of the fungi species involved in the formation of those mycotic skin diseases colonize primarily the stratum corneum (SC), studying the interaction between fengycin and SC-mimicking lipid membranes is a primary step to determine the potential of fengycin to overcome the physical barrier of the skin. In this respect, multilamellar lipid vesicles (MLVs), with a lipid composition mimicking that of the SC, were prepared and characterized by differential scanning calorimetry (DSC). The critical micelle concentration (CMC) of fengycin was also assessed under skin conditions and found... (More)
Based on its outstanding antifungal properties, it is reasonable to believe that fengycin might be efficient to topically treat localized dermatomycoses. Since most of the fungi species involved in the formation of those mycotic skin diseases colonize primarily the stratum corneum (SC), studying the interaction between fengycin and SC-mimicking lipid membranes is a primary step to determine the potential of fengycin to overcome the physical barrier of the skin. In this respect, multilamellar lipid vesicles (MLVs), with a lipid composition mimicking that of the SC, were prepared and characterized by differential scanning calorimetry (DSC). The critical micelle concentration (CMC) of fengycin was also assessed under skin conditions and found to be 1.2±0.1μM. The molecular interactions of fengycin with SC-mimicking MLVs were investigated by both DSC and isothermal titration calorimetry (ITC). Results showed that the interactions were considerably affected by changes in lipid phase behaviour. At 40°C and below, fengycin induced exothermic changes in the lipid structures suggesting that less-ordered lipid domains became more-ordered in presence of fengycin. At 60°C, clearly endothermic interaction enthalpies were observed, which could arise from the "melting" of remaining solid domains enriched in high melting lipids that without fengycin melt at higher temperatures. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces B: Biointerfaces
volume
121
pages
27 - 35
publisher
Elsevier
external identifiers
  • pmid:24929530
  • wos:000341335800004
  • scopus:84902470115
ISSN
1873-4367
DOI
10.1016/j.colsurfb.2014.05.019
language
English
LU publication?
yes
id
57b64016-d794-41a1-a188-2e968875d34c (old id 4528453)
date added to LUP
2014-08-08 15:57:43
date last changed
2017-06-11 03:14:14
@article{57b64016-d794-41a1-a188-2e968875d34c,
  abstract     = {Based on its outstanding antifungal properties, it is reasonable to believe that fengycin might be efficient to topically treat localized dermatomycoses. Since most of the fungi species involved in the formation of those mycotic skin diseases colonize primarily the stratum corneum (SC), studying the interaction between fengycin and SC-mimicking lipid membranes is a primary step to determine the potential of fengycin to overcome the physical barrier of the skin. In this respect, multilamellar lipid vesicles (MLVs), with a lipid composition mimicking that of the SC, were prepared and characterized by differential scanning calorimetry (DSC). The critical micelle concentration (CMC) of fengycin was also assessed under skin conditions and found to be 1.2±0.1μM. The molecular interactions of fengycin with SC-mimicking MLVs were investigated by both DSC and isothermal titration calorimetry (ITC). Results showed that the interactions were considerably affected by changes in lipid phase behaviour. At 40°C and below, fengycin induced exothermic changes in the lipid structures suggesting that less-ordered lipid domains became more-ordered in presence of fengycin. At 60°C, clearly endothermic interaction enthalpies were observed, which could arise from the "melting" of remaining solid domains enriched in high melting lipids that without fengycin melt at higher temperatures.},
  author       = {Eeman, Marc and Olofsson, Gerd and Sparr, Emma and Nasir, Mehmet Nail and Nylander, Tommy and Deleu, Magali},
  issn         = {1873-4367},
  language     = {eng},
  pages        = {27--35},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Interaction of fengycin with stratum corneum mimicking model membranes: A calorimetry study.},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2014.05.019},
  volume       = {121},
  year         = {2014},
}