Uridine triphosphate (UTP) induces profibrotic responses in cardiac fibroblasts by activation of P2Y2 receptors
(2010) In Journal of Molecular and Cellular Cardiology 49(3). p.9-362- Abstract
Cardiac fibroblasts (CFs) play a key role in response to injury and remodeling of the heart. Nucleotide (P2) receptors regulate the heart but limited information is available regarding such receptors in CFs. We thus sought to determine if extracellular nucleotides regulate fibrotic responses (e.g., proliferation, migration and expression of profibrotic markers) of CFs in primary culture. UTP increased rat CF migration 3-fold (p<0.001), proliferation by 30% (p<0.05) and mRNA expression of profibrotic markers: alpha smooth muscle actin (alpha-SMA), plasminogen activator inhibitor-1 (PAI-1), transforming growth factor beta, soluble ST2, interleukin-6 and monocyte chemoattractant protein-1 (MCP-1) by 3.0-, 15-, 2.0-, 7.6-, 11-, and... (More)
Cardiac fibroblasts (CFs) play a key role in response to injury and remodeling of the heart. Nucleotide (P2) receptors regulate the heart but limited information is available regarding such receptors in CFs. We thus sought to determine if extracellular nucleotides regulate fibrotic responses (e.g., proliferation, migration and expression of profibrotic markers) of CFs in primary culture. UTP increased rat CF migration 3-fold (p<0.001), proliferation by 30% (p<0.05) and mRNA expression of profibrotic markers: alpha smooth muscle actin (alpha-SMA), plasminogen activator inhibitor-1 (PAI-1), transforming growth factor beta, soluble ST2, interleukin-6 and monocyte chemoattractant protein-1 (MCP-1) by 3.0-, 15-, 2.0-, 7.6-, 11-, and 6.1-fold, respectively (p<0.05). PAI-1 protein expression induced by UTP was dependent on protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), based on blockade by the PKC inhibitor Ro-31-8220 and the ERK inhibitor U0126, respectively. The rank order for enhanced expression of PAI-1 and alpha-SMA by nucleotides (UTPgammaS>UDPbetaS>ATPgammaS), the expression of P2Y2 receptors as the most abundantly expressed P2Y receptor in rat CFs and a blunted response to UTP in P2Y2(-/-) mice all implicate P2Y2 as the predominant P2Y receptor that mediates nucleotide-promoted profibrotic responses. Additional results indicate that P2Y2 receptor-promoted profibrotic responses in CFs are transient, perhaps as a consequence of receptor desensitization. We conclude that P2Y2 receptor activation is profibrotic in CFs; thus inhibition of P2Y2 receptors may provide a novel means to diminish fibrotic remodeling and turnover of extracellular matrix in the heart.
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- author
- Braun, Oscar O LU ; Lu, David ; Aroonsakool, Nakon and Insel, Paul A
- publishing date
- 2010-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Blotting, Western, Cell Movement/drug effects, Cell Proliferation/drug effects, Cells, Cultured, Chemokine CCL2/genetics, Extracellular Signal-Regulated MAP Kinases/genetics, Fibroblasts/drug effects, Flow Cytometry, Fluorescent Antibody Technique, Heart/drug effects, Interleukin-6/genetics, Male, Mice, Mice, Inbred C57BL, Mitogen-Activated Protein Kinases/genetics, Plasminogen Activator Inhibitor 1/genetics, Protein Kinase C/genetics, RNA, Messenger/genetics, Rats, Rats, Sprague-Dawley, Receptors, Purinergic P2Y2/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Uridine Triphosphate/pharmacology
- in
- Journal of Molecular and Cellular Cardiology
- volume
- 49
- issue
- 3
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:20471392
- scopus:77955272897
- ISSN
- 1095-8584
- DOI
- 10.1016/j.yjmcc.2010.05.001
- language
- English
- LU publication?
- no
- additional info
- Copyright 2010 Elsevier Ltd. All rights reserved.
- id
- 4529e470-624d-42be-972e-aac1e4fbdab8
- alternative location
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2917486/
- date added to LUP
- 2019-06-18 22:36:11
- date last changed
- 2024-01-01 11:04:05
@article{4529e470-624d-42be-972e-aac1e4fbdab8,
abstract = {{<p>Cardiac fibroblasts (CFs) play a key role in response to injury and remodeling of the heart. Nucleotide (P2) receptors regulate the heart but limited information is available regarding such receptors in CFs. We thus sought to determine if extracellular nucleotides regulate fibrotic responses (e.g., proliferation, migration and expression of profibrotic markers) of CFs in primary culture. UTP increased rat CF migration 3-fold (p<0.001), proliferation by 30% (p<0.05) and mRNA expression of profibrotic markers: alpha smooth muscle actin (alpha-SMA), plasminogen activator inhibitor-1 (PAI-1), transforming growth factor beta, soluble ST2, interleukin-6 and monocyte chemoattractant protein-1 (MCP-1) by 3.0-, 15-, 2.0-, 7.6-, 11-, and 6.1-fold, respectively (p<0.05). PAI-1 protein expression induced by UTP was dependent on protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), based on blockade by the PKC inhibitor Ro-31-8220 and the ERK inhibitor U0126, respectively. The rank order for enhanced expression of PAI-1 and alpha-SMA by nucleotides (UTPgammaS>UDPbetaS>ATPgammaS), the expression of P2Y2 receptors as the most abundantly expressed P2Y receptor in rat CFs and a blunted response to UTP in P2Y2(-/-) mice all implicate P2Y2 as the predominant P2Y receptor that mediates nucleotide-promoted profibrotic responses. Additional results indicate that P2Y2 receptor-promoted profibrotic responses in CFs are transient, perhaps as a consequence of receptor desensitization. We conclude that P2Y2 receptor activation is profibrotic in CFs; thus inhibition of P2Y2 receptors may provide a novel means to diminish fibrotic remodeling and turnover of extracellular matrix in the heart.</p>}},
author = {{Braun, Oscar O and Lu, David and Aroonsakool, Nakon and Insel, Paul A}},
issn = {{1095-8584}},
keywords = {{Animals; Blotting, Western; Cell Movement/drug effects; Cell Proliferation/drug effects; Cells, Cultured; Chemokine CCL2/genetics; Extracellular Signal-Regulated MAP Kinases/genetics; Fibroblasts/drug effects; Flow Cytometry; Fluorescent Antibody Technique; Heart/drug effects; Interleukin-6/genetics; Male; Mice; Mice, Inbred C57BL; Mitogen-Activated Protein Kinases/genetics; Plasminogen Activator Inhibitor 1/genetics; Protein Kinase C/genetics; RNA, Messenger/genetics; Rats; Rats, Sprague-Dawley; Receptors, Purinergic P2Y2/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Uridine Triphosphate/pharmacology}},
language = {{eng}},
number = {{3}},
pages = {{9--362}},
publisher = {{Elsevier}},
series = {{Journal of Molecular and Cellular Cardiology}},
title = {{Uridine triphosphate (UTP) induces profibrotic responses in cardiac fibroblasts by activation of P2Y2 receptors}},
url = {{http://dx.doi.org/10.1016/j.yjmcc.2010.05.001}},
doi = {{10.1016/j.yjmcc.2010.05.001}},
volume = {{49}},
year = {{2010}},
}