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Reduced acoustic resonator dimensions improve focusing efficiency of bacteria and submicron particles

Ugawa, Masashi ; Lee, Hoyeon ; Baasch, Thierry LU ; Lee, Minho ; Kim, Soyun ; Jeong, OkChan ; Choi, Yong-Hoon ; Sohn, Daewon ; Laurell, Thomas LU and Ota, Sadao , et al. (2022) In Analyst 147(2). p.274-281
Abstract
In this study, we demonstrate an acoustofluidic device that enables single-file focusing of submicron particles and bacteria using a two-dimensional (2D) acoustic standing wave. The device consists of a 100 μm × 100 μm square channel that supports 2D particle focusing in the channel center at an actuation frequency of 7.39 MHz. This higher actuation frequency compared with conventional bulk acoustic systems enables radiation-force-dominant motion of submicron particles and overcomes the classical size limitation (≈2 μm) of acoustic focusing. We present acoustic radiation force-based focusing of particles with diameters less than 0.5 μm at a flow rate of 12 μL min−1, and 1.33 μm particles at flow rates up to 80 μL min−1. The device focused... (More)
In this study, we demonstrate an acoustofluidic device that enables single-file focusing of submicron particles and bacteria using a two-dimensional (2D) acoustic standing wave. The device consists of a 100 μm × 100 μm square channel that supports 2D particle focusing in the channel center at an actuation frequency of 7.39 MHz. This higher actuation frequency compared with conventional bulk acoustic systems enables radiation-force-dominant motion of submicron particles and overcomes the classical size limitation (≈2 μm) of acoustic focusing. We present acoustic radiation force-based focusing of particles with diameters less than 0.5 μm at a flow rate of 12 μL min−1, and 1.33 μm particles at flow rates up to 80 μL min−1. The device focused 0.25 μm particles by the 2D acoustic radiation force while undergoing a channel cross-section centered, single-vortex acoustic streaming. A suspension of bacteria was also investigated to evaluate the biological relevance of the device, which demonstrated the alignment of bacteria in the channel at a
flow rate of up to 20 μL min−1. The developed acoustofluidic device can align submicron particles within a narrow flow stream in a highly robust manner, validating its use as a flow-through focusing chamber to perform high-throughput and accurate flow cytometry of submicron objects (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analyst
volume
147
issue
2
pages
8 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85123499152
  • pmid:34889326
ISSN
1364-5528
DOI
10.1039/d1an01891d
language
English
LU publication?
yes
id
45800fac-6c0c-424a-b4a6-b68bbdb1989a
date added to LUP
2022-02-08 19:24:34
date last changed
2023-11-08 13:11:21
@article{45800fac-6c0c-424a-b4a6-b68bbdb1989a,
  abstract     = {{In this study, we demonstrate an acoustofluidic device that enables single-file focusing of submicron particles and bacteria using a two-dimensional (2D) acoustic standing wave. The device consists of a 100 μm × 100 μm square channel that supports 2D particle focusing in the channel center at an actuation frequency of 7.39 MHz. This higher actuation frequency compared with conventional bulk acoustic systems enables radiation-force-dominant motion of submicron particles and overcomes the classical size limitation (≈2 μm) of acoustic focusing. We present acoustic radiation force-based focusing of particles with diameters less than 0.5 μm at a flow rate of 12 μL min−1, and 1.33 μm particles at flow rates up to 80 μL min−1. The device focused 0.25 μm particles by the 2D acoustic radiation force while undergoing a channel cross-section centered, single-vortex acoustic streaming. A suspension of bacteria was also investigated to evaluate the biological relevance of the device, which demonstrated the alignment of bacteria in the channel at a<br/>flow rate of up to 20 μL min−1. The developed acoustofluidic device can align submicron particles within a narrow flow stream in a highly robust manner, validating its use as a flow-through focusing chamber to perform high-throughput and accurate flow cytometry of submicron objects}},
  author       = {{Ugawa, Masashi and Lee, Hoyeon and Baasch, Thierry and Lee, Minho and Kim, Soyun and Jeong, OkChan and Choi, Yong-Hoon and Sohn, Daewon and Laurell, Thomas and Ota, Sadao and Lee, SangWook}},
  issn         = {{1364-5528}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{274--281}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Analyst}},
  title        = {{Reduced acoustic resonator dimensions improve focusing efficiency of bacteria and submicron particles}},
  url          = {{http://dx.doi.org/10.1039/d1an01891d}},
  doi          = {{10.1039/d1an01891d}},
  volume       = {{147}},
  year         = {{2022}},
}