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Acoustic radiation forces at liquid interfaces impact the performance of acoustophoresis.

Deshmukh, Sameer; Brzozka, Zbigniew; Laurell, Thomas LU and Augustsson, Per LU (2014) In Lab on A Chip 14(17). p.3394-3400
Abstract
Acoustophoresis is a method well suited for cell and microbead separation or concentration for downstream analysis in microfluidic settings. One of the main limitations that acoustophoresis share with other microfluidic techniques is that the separation efficiency is poor for particle-rich suspensions. We report that flow laminated liquids can be relocated in a microchannel when exposed to a resonant acoustic field. Differences in acoustic impedance between two liquids cause migration of the high-impedance liquid towards an acoustic pressure node. In a set of experiments we charted this phenomenon and show herein that it can be used to either relocate liquids with respect to each other, or to stabilize the interface between them. This... (More)
Acoustophoresis is a method well suited for cell and microbead separation or concentration for downstream analysis in microfluidic settings. One of the main limitations that acoustophoresis share with other microfluidic techniques is that the separation efficiency is poor for particle-rich suspensions. We report that flow laminated liquids can be relocated in a microchannel when exposed to a resonant acoustic field. Differences in acoustic impedance between two liquids cause migration of the high-impedance liquid towards an acoustic pressure node. In a set of experiments we charted this phenomenon and show herein that it can be used to either relocate liquids with respect to each other, or to stabilize the interface between them. This resulted in decreased medium carry-over when transferring microbeads (4% by volume) between suspending liquids using acoustophoresis. Furthermore we demonstrate that acoustic relocation of liquids occurs for impedance differences as low as 0.1%. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
14
issue
17
pages
3394 - 3400
publisher
Royal Society of Chemistry
external identifiers
  • pmid:25007385
  • wos:000340204300034
  • scopus:84905454530
ISSN
1473-0189
DOI
10.1039/c4lc00572d
language
English
LU publication?
yes
id
4a1f9cf3-934a-4be3-b0c8-a88e3127f698 (old id 4583225)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25007385?dopt=Abstract
date added to LUP
2014-08-07 19:34:13
date last changed
2017-09-10 03:22:12
@article{4a1f9cf3-934a-4be3-b0c8-a88e3127f698,
  abstract     = {Acoustophoresis is a method well suited for cell and microbead separation or concentration for downstream analysis in microfluidic settings. One of the main limitations that acoustophoresis share with other microfluidic techniques is that the separation efficiency is poor for particle-rich suspensions. We report that flow laminated liquids can be relocated in a microchannel when exposed to a resonant acoustic field. Differences in acoustic impedance between two liquids cause migration of the high-impedance liquid towards an acoustic pressure node. In a set of experiments we charted this phenomenon and show herein that it can be used to either relocate liquids with respect to each other, or to stabilize the interface between them. This resulted in decreased medium carry-over when transferring microbeads (4% by volume) between suspending liquids using acoustophoresis. Furthermore we demonstrate that acoustic relocation of liquids occurs for impedance differences as low as 0.1%.},
  author       = {Deshmukh, Sameer and Brzozka, Zbigniew and Laurell, Thomas and Augustsson, Per},
  issn         = {1473-0189},
  language     = {eng},
  number       = {17},
  pages        = {3394--3400},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Acoustic radiation forces at liquid interfaces impact the performance of acoustophoresis.},
  url          = {http://dx.doi.org/10.1039/c4lc00572d},
  volume       = {14},
  year         = {2014},
}