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Identification and qualitative characterization of high and low lignin lines from an oat TILLING population

Vivekanand, Vivekanand; Chawade, Aakash LU ; Larsson, Mikael; Larsson, Anette and Olsson, Olof LU (2014) In Industrial Crops and Products 59. p.1-8
Abstract
To identify differences in seed lignin content, 520 randomly chosen independent lines were screened in mutagenized oat population and lines with the seed lignin levels ranging from 20 to 63 gkg(-1) were identified. In the commercial variety Belinda, from which the mutated population was developed, seed lignin level was determined to be 41 g kg(-1). In Assiniboia, a Canadian low lignin variety, it was found to be 21 g kg(-1). To investigate if extracted lignin from the mutated lines were also qualitatively different from Belinda, two lines with the lowest and highest lignin levels were selected for structural analyses using XRD, UV and FT-IR spectroscopy. Results showed that there were significant qualitative differences in seed lignin in... (More)
To identify differences in seed lignin content, 520 randomly chosen independent lines were screened in mutagenized oat population and lines with the seed lignin levels ranging from 20 to 63 gkg(-1) were identified. In the commercial variety Belinda, from which the mutated population was developed, seed lignin level was determined to be 41 g kg(-1). In Assiniboia, a Canadian low lignin variety, it was found to be 21 g kg(-1). To investigate if extracted lignin from the mutated lines were also qualitatively different from Belinda, two lines with the lowest and highest lignin levels were selected for structural analyses using XRD, UV and FT-IR spectroscopy. Results showed that there were significant qualitative differences in seed lignin in the mutated lines and in Belinda, and based on this, we predict that lignin from the mutated seeds will be more digestible in ruminant animals than Belinda seeds. This prediction was confirmed in preliminary in vitro digestion experiments. (C) 2014 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Oat, Lignin, Belinda, Assiniboia, Dioxane, UV, FT-IR spectroscopy
in
Industrial Crops and Products
volume
59
pages
1 - 8
publisher
Elsevier
external identifiers
  • wos:000339600400001
  • scopus:84900793828
ISSN
0926-6690
DOI
10.1016/j.indcrop.2014.04.019
language
English
LU publication?
yes
id
6b1c230e-9d0e-4ae0-971f-09ef01e4cdb3 (old id 4590603)
date added to LUP
2014-09-04 11:13:11
date last changed
2017-10-29 04:00:51
@article{6b1c230e-9d0e-4ae0-971f-09ef01e4cdb3,
  abstract     = {To identify differences in seed lignin content, 520 randomly chosen independent lines were screened in mutagenized oat population and lines with the seed lignin levels ranging from 20 to 63 gkg(-1) were identified. In the commercial variety Belinda, from which the mutated population was developed, seed lignin level was determined to be 41 g kg(-1). In Assiniboia, a Canadian low lignin variety, it was found to be 21 g kg(-1). To investigate if extracted lignin from the mutated lines were also qualitatively different from Belinda, two lines with the lowest and highest lignin levels were selected for structural analyses using XRD, UV and FT-IR spectroscopy. Results showed that there were significant qualitative differences in seed lignin in the mutated lines and in Belinda, and based on this, we predict that lignin from the mutated seeds will be more digestible in ruminant animals than Belinda seeds. This prediction was confirmed in preliminary in vitro digestion experiments. (C) 2014 Elsevier B.V. All rights reserved.},
  author       = {Vivekanand, Vivekanand and Chawade, Aakash and Larsson, Mikael and Larsson, Anette and Olsson, Olof},
  issn         = {0926-6690},
  keyword      = {Oat,Lignin,Belinda,Assiniboia,Dioxane,UV,FT-IR spectroscopy},
  language     = {eng},
  pages        = {1--8},
  publisher    = {Elsevier},
  series       = {Industrial Crops and Products},
  title        = {Identification and qualitative characterization of high and low lignin lines from an oat TILLING population},
  url          = {http://dx.doi.org/10.1016/j.indcrop.2014.04.019},
  volume       = {59},
  year         = {2014},
}