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Human Lipoproteins at Model Cell Membranes : Effect of Lipoprotein Class on Lipid Exchange

Browning, K. L.; Lind, T. K.; Maric, S.; Malekkhaiat-Häffner, S.; Fredrikson, G. N. LU ; Bengtsson, E. LU ; Malmsten, M. and Cárdenas, M. LU (2017) In Scientific Reports 7(1).
Abstract

High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL... (More)

High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the 'good' cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the 'bad' cholesterol, depositing lipid material into the vascular wall.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
7
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85027222137
  • wos:000407080100108
ISSN
2045-2322
DOI
10.1038/s41598-017-07505-0
language
English
LU publication?
yes
id
45ebebb3-59c1-410a-9ff7-163a4de3a416
date added to LUP
2017-08-24 15:03:17
date last changed
2017-09-18 11:42:59
@article{45ebebb3-59c1-410a-9ff7-163a4de3a416,
  abstract     = {<p>High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the 'good' cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the 'bad' cholesterol, depositing lipid material into the vascular wall.</p>},
  articleno    = {7478},
  author       = {Browning, K. L. and Lind, T. K. and Maric, S. and Malekkhaiat-Häffner, S. and Fredrikson, G. N. and Bengtsson, E. and Malmsten, M. and Cárdenas, M.},
  issn         = {2045-2322},
  language     = {eng},
  month        = {08},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Human Lipoproteins at Model Cell Membranes : Effect of Lipoprotein Class on Lipid Exchange},
  url          = {http://dx.doi.org/10.1038/s41598-017-07505-0},
  volume       = {7},
  year         = {2017},
}