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Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature

Lind, Tania Kjellerup; Cardenas, Marite and Wacklin, Hanna LU (2014) In Langmuir 30(25). p.7259-7263
Abstract
We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly... (More)
We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid T-m. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
30
issue
25
pages
7259 - 7263
publisher
The American Chemical Society
external identifiers
  • wos:000338488600001
  • scopus:84903748464
ISSN
0743-7463
DOI
10.1021/la500897x
language
English
LU publication?
yes
id
46002feb-e68a-4297-acc1-c62ba0a8ec8d (old id 4602814)
date added to LUP
2014-09-04 12:57:36
date last changed
2017-11-12 03:06:18
@article{46002feb-e68a-4297-acc1-c62ba0a8ec8d,
  abstract     = {We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid T-m. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported.},
  author       = {Lind, Tania Kjellerup and Cardenas, Marite and Wacklin, Hanna},
  issn         = {0743-7463},
  language     = {eng},
  number       = {25},
  pages        = {7259--7263},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature},
  url          = {http://dx.doi.org/10.1021/la500897x},
  volume       = {30},
  year         = {2014},
}