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A porous silicon immunoassay platform for fluorometric determination of alpha-synuclein in human cerebrospinal fluid

Lee, Sangwook; Silajdzic, Edina LU ; Yang, Hon; Björkqvist, Maria LU ; Kim, Soyon; Jeong, Ok Chan; Hansson, Oskar LU and Laurell, Thomas LU (2014) In Mikrochimica Acta 181(9-10). p.1143-1149
Abstract
Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only... (More)
Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only (including immobilization of capturing antibody). The limit of detection is 35 pg mL(-1) of alpha-synuclein in cerebrospinal fluid, and the dynamic analytical range extends from 0.01 to 100 ng center dot mL(-1). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Porous silicon, Antibody microarray, alpha-synuclein, Parkinson's, disease
in
Mikrochimica Acta
volume
181
issue
9-10
pages
1143 - 1149
publisher
Springer
external identifiers
  • wos:000338317000034
  • scopus:84903199111
ISSN
1436-5073
DOI
10.1007/s00604-014-1180-2
language
English
LU publication?
yes
id
7ea5c63c-7b82-497d-9cda-fc7a74c1263f (old id 4602824)
date added to LUP
2014-09-04 13:30:05
date last changed
2017-08-20 03:03:19
@article{7ea5c63c-7b82-497d-9cda-fc7a74c1263f,
  abstract     = {Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only (including immobilization of capturing antibody). The limit of detection is 35 pg mL(-1) of alpha-synuclein in cerebrospinal fluid, and the dynamic analytical range extends from 0.01 to 100 ng center dot mL(-1).},
  author       = {Lee, Sangwook and Silajdzic, Edina and Yang, Hon and Björkqvist, Maria and Kim, Soyon and Jeong, Ok Chan and Hansson, Oskar and Laurell, Thomas},
  issn         = {1436-5073},
  keyword      = {Porous silicon,Antibody microarray,alpha-synuclein,Parkinson's,disease},
  language     = {eng},
  number       = {9-10},
  pages        = {1143--1149},
  publisher    = {Springer},
  series       = {Mikrochimica Acta},
  title        = {A porous silicon immunoassay platform for fluorometric determination of alpha-synuclein in human cerebrospinal fluid},
  url          = {http://dx.doi.org/10.1007/s00604-014-1180-2},
  volume       = {181},
  year         = {2014},
}