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Preparation of two glycoside hydrolases for use in micro-aqueous media

Lundemo, Pontus LU ; Nordberg Karlsson, Eva LU and Adlercreutz, Patrick LU (2014) In Journal of Molecular Catalysis B: Enzymatic 108. p.1-6
Abstract
Enzymatic synthesis of alkyl glycosides using glycoside hydrolases is well studied, but has yet to reach industrial scale, primarily due to limited yields. Reduced water content should increase yields by limiting the unwanted hydrolytic side reaction. However, previous studies have shown that a reduction in water content surprisingly favors hydrolysis over transglycosylation. In addition, glycoside hydrolases normally require a high degree of hydration to function efficiently. This study compares six enzyme preparation methods to improve resilience and activity of two glycoside hydrolases from Thermotoga neapolitana (TnBgl3B and TnBgl1A) in micro-aqueous hexanol. Indeed, when adsorbed onto Accurel MP-1000 both enzymes increasingly favored... (More)
Enzymatic synthesis of alkyl glycosides using glycoside hydrolases is well studied, but has yet to reach industrial scale, primarily due to limited yields. Reduced water content should increase yields by limiting the unwanted hydrolytic side reaction. However, previous studies have shown that a reduction in water content surprisingly favors hydrolysis over transglycosylation. In addition, glycoside hydrolases normally require a high degree of hydration to function efficiently. This study compares six enzyme preparation methods to improve resilience and activity of two glycoside hydrolases from Thermotoga neapolitana (TnBgl3B and TnBgl1A) in micro-aqueous hexanol. Indeed, when adsorbed onto Accurel MP-1000 both enzymes increasingly favored transglycosylation over hydrolysis at low hydration, in contrast to freeze-dried or untreated enzyme. Additionally, they displayed 17–70× higher reaction rates compared to freeze-dried enzyme at low water activity, while displaying comparable or lower activity for fully hydrated systems. These results provide valuable information for use of enzymes under micro-aqueous conditions and build toward utilizing the full synthetic potential of glycoside hydrolases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Transglycosylation, Hydrolase, Immobilization, Organic solvent, Alkyl glycoside
in
Journal of Molecular Catalysis B: Enzymatic
volume
108
pages
1 - 6
publisher
Elsevier
external identifiers
  • wos:000341548800001
  • scopus:84904191330
ISSN
1873-3158
DOI
10.1016/j.molcatb.2014.06.009
language
English
LU publication?
yes
id
0648b9be-96bf-461a-b8e2-3aa7ccb4cbee (old id 4611413)
date added to LUP
2014-10-07 17:46:18
date last changed
2017-02-05 03:08:50
@article{0648b9be-96bf-461a-b8e2-3aa7ccb4cbee,
  abstract     = {Enzymatic synthesis of alkyl glycosides using glycoside hydrolases is well studied, but has yet to reach industrial scale, primarily due to limited yields. Reduced water content should increase yields by limiting the unwanted hydrolytic side reaction. However, previous studies have shown that a reduction in water content surprisingly favors hydrolysis over transglycosylation. In addition, glycoside hydrolases normally require a high degree of hydration to function efficiently. This study compares six enzyme preparation methods to improve resilience and activity of two glycoside hydrolases from Thermotoga neapolitana (TnBgl3B and TnBgl1A) in micro-aqueous hexanol. Indeed, when adsorbed onto Accurel MP-1000 both enzymes increasingly favored transglycosylation over hydrolysis at low hydration, in contrast to freeze-dried or untreated enzyme. Additionally, they displayed 17–70× higher reaction rates compared to freeze-dried enzyme at low water activity, while displaying comparable or lower activity for fully hydrated systems. These results provide valuable information for use of enzymes under micro-aqueous conditions and build toward utilizing the full synthetic potential of glycoside hydrolases.},
  author       = {Lundemo, Pontus and Nordberg Karlsson, Eva and Adlercreutz, Patrick},
  issn         = {1873-3158},
  keyword      = {Transglycosylation,Hydrolase,Immobilization,Organic solvent,Alkyl glycoside},
  language     = {eng},
  pages        = {1--6},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Catalysis B: Enzymatic},
  title        = {Preparation of two glycoside hydrolases for use in micro-aqueous media},
  url          = {http://dx.doi.org/10.1016/j.molcatb.2014.06.009},
  volume       = {108},
  year         = {2014},
}