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Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore.

Niksirat, Hamid; Andersson, Liselotte LU ; James, Peter LU ; Kouba, Antonín and Kozák, Pavel (2014) In Animal Reproduction Science 149(3-4). p.335-344
Abstract
Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity... (More)
Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity and cell defence categories in the protein profile of the crayfish egg. Differences between protein profiles of the crayfish egg and spermatophore show different functional priorities for each of gametes. Several proteins having possible roles in gametogenesis, capacitation, acrosome reaction, and fertilization were identified. This proteomic profile of signal crayfish gametes provides a basis for further investigation of functional roles of the identified proteins in aspects of reproduction such as capacitation and fertilization. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Animal Reproduction Science
volume
149
issue
3-4
pages
335 - 344
publisher
Elsevier
external identifiers
  • wos:000343620200032
  • pmid:25156317
  • scopus:84910089258
ISSN
1873-2232
DOI
10.1016/j.anireprosci.2014.07.024
language
English
LU publication?
yes
id
9996419c-ff41-4c2c-814d-f42756d69798 (old id 4613915)
date added to LUP
2014-09-11 16:56:14
date last changed
2017-10-29 03:00:45
@article{9996419c-ff41-4c2c-814d-f42756d69798,
  abstract     = {Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity and cell defence categories in the protein profile of the crayfish egg. Differences between protein profiles of the crayfish egg and spermatophore show different functional priorities for each of gametes. Several proteins having possible roles in gametogenesis, capacitation, acrosome reaction, and fertilization were identified. This proteomic profile of signal crayfish gametes provides a basis for further investigation of functional roles of the identified proteins in aspects of reproduction such as capacitation and fertilization.},
  author       = {Niksirat, Hamid and Andersson, Liselotte and James, Peter and Kouba, Antonín and Kozák, Pavel},
  issn         = {1873-2232},
  language     = {eng},
  number       = {3-4},
  pages        = {335--344},
  publisher    = {Elsevier},
  series       = {Animal Reproduction Science},
  title        = {Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore.},
  url          = {http://dx.doi.org/10.1016/j.anireprosci.2014.07.024},
  volume       = {149},
  year         = {2014},
}