Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore.
Niksirat, Hamid ; Andersson, Liselotte LU ; James, Peter LU
; Kouba, Antonín
and Kozák, Pavel
(2014)
In Animal Reproduction Science
149(3-4).
p.335-344
- Abstract
- Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity... (More)
- Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity and cell defence categories in the protein profile of the crayfish egg. Differences between protein profiles of the crayfish egg and spermatophore show different functional priorities for each of gametes. Several proteins having possible roles in gametogenesis, capacitation, acrosome reaction, and fertilization were identified. This proteomic profile of signal crayfish gametes provides a basis for further investigation of functional roles of the identified proteins in aspects of reproduction such as capacitation and fertilization. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4613915
- author
- Niksirat, Hamid
; Andersson, Liselotte
LU
; James, Peter
LU
; Kouba, Antonín
and Kozák, Pavel
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Animal Reproduction Science
- volume
- 149
- issue
- 3-4
- pages
- 335 - 344
- publisher
- Elsevier
- external identifiers
-
- wos:000343620200032
- pmid:25156317
- scopus:84910089258
- pmid:25156317
- ISSN
- 1873-2232
- DOI
- 10.1016/j.anireprosci.2014.07.024
- language
- English
- LU publication?
- yes
- id
- 9996419c-ff41-4c2c-814d-f42756d69798 (old id 4613915)
- date added to LUP
- 2016-04-01 09:50:06
- date last changed
- 2023-11-09 05:26:24
@article{9996419c-ff41-4c2c-814d-f42756d69798,
abstract = {{Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity and cell defence categories in the protein profile of the crayfish egg. Differences between protein profiles of the crayfish egg and spermatophore show different functional priorities for each of gametes. Several proteins having possible roles in gametogenesis, capacitation, acrosome reaction, and fertilization were identified. This proteomic profile of signal crayfish gametes provides a basis for further investigation of functional roles of the identified proteins in aspects of reproduction such as capacitation and fertilization.}},
author = {{Niksirat, Hamid and Andersson, Liselotte and James, Peter and Kouba, Antonín and Kozák, Pavel}},
issn = {{1873-2232}},
language = {{eng}},
number = {{3-4}},
pages = {{335--344}},
publisher = {{Elsevier}},
series = {{Animal Reproduction Science}},
title = {{Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore.}},
url = {{http://dx.doi.org/10.1016/j.anireprosci.2014.07.024}},
doi = {{10.1016/j.anireprosci.2014.07.024}},
volume = {{149}},
year = {{2014}},
}