PAPST1 regulates sulfation of heparan sulfate proteoglycans in epithelial MDCK II cells.
(2015) In Glycobiology 25(1). p.30-41- Abstract
- Proteoglycan (PG) sulfation depends on activated nucleotide sulfate, 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Transporters in the Golgi membrane translocate PAPS from the cytoplasm into the organelle lumen where PG sulfation occurs. Silencing of PAPS transporter (PAPST) 1 in epithelial MDCK cells reduced PAPS uptake into Golgi vesicles. Surprisingly, at the same time sulfation of heparan sulfate (HS) was stimulated. The effect was pathway specific in polarized epithelial cells. Basolaterally secreted PGs displayed an altered HS sulfation pattern and increased growth factor binding capacity. In contrast, the sulfation pattern of apically secreted PGs was unchanged while the secretion was reduced. Regulation of PAPST1 allows epithelial... (More)
- Proteoglycan (PG) sulfation depends on activated nucleotide sulfate, 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Transporters in the Golgi membrane translocate PAPS from the cytoplasm into the organelle lumen where PG sulfation occurs. Silencing of PAPS transporter (PAPST) 1 in epithelial MDCK cells reduced PAPS uptake into Golgi vesicles. Surprisingly, at the same time sulfation of heparan sulfate (HS) was stimulated. The effect was pathway specific in polarized epithelial cells. Basolaterally secreted PGs displayed an altered HS sulfation pattern and increased growth factor binding capacity. In contrast, the sulfation pattern of apically secreted PGs was unchanged while the secretion was reduced. Regulation of PAPST1 allows epithelial cells to prioritize between PG sulfation in the apical and basolateral secretory routes at the level of the Golgi apparatus. This provides sulfation patterns that ensure PG functions at the extracellular level, such as growth factor binding. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4614311
- author
- Dick, Gunnar ; Akslen-Hoel, Linn Kristin ; Grøndahl, Frøy ; Kjos, Ingrid ; Maccarana, Marco LU and Prydz, Kristian
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Glycobiology
- volume
- 25
- issue
- 1
- pages
- 30 - 41
- publisher
- Oxford University Press
- external identifiers
-
- pmid:25138304
- wos:000347410500004
- scopus:84924941678
- pmid:25138304
- ISSN
- 1460-2423
- DOI
- 10.1093/glycob/cwu084
- language
- English
- LU publication?
- yes
- id
- 6387ac4c-6771-45d1-b471-7a132dd67c67 (old id 4614311)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25138304?dopt=Abstract
- date added to LUP
- 2016-04-01 10:40:59
- date last changed
- 2022-04-12 08:36:51
@article{6387ac4c-6771-45d1-b471-7a132dd67c67, abstract = {{Proteoglycan (PG) sulfation depends on activated nucleotide sulfate, 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Transporters in the Golgi membrane translocate PAPS from the cytoplasm into the organelle lumen where PG sulfation occurs. Silencing of PAPS transporter (PAPST) 1 in epithelial MDCK cells reduced PAPS uptake into Golgi vesicles. Surprisingly, at the same time sulfation of heparan sulfate (HS) was stimulated. The effect was pathway specific in polarized epithelial cells. Basolaterally secreted PGs displayed an altered HS sulfation pattern and increased growth factor binding capacity. In contrast, the sulfation pattern of apically secreted PGs was unchanged while the secretion was reduced. Regulation of PAPST1 allows epithelial cells to prioritize between PG sulfation in the apical and basolateral secretory routes at the level of the Golgi apparatus. This provides sulfation patterns that ensure PG functions at the extracellular level, such as growth factor binding.}}, author = {{Dick, Gunnar and Akslen-Hoel, Linn Kristin and Grøndahl, Frøy and Kjos, Ingrid and Maccarana, Marco and Prydz, Kristian}}, issn = {{1460-2423}}, language = {{eng}}, number = {{1}}, pages = {{30--41}}, publisher = {{Oxford University Press}}, series = {{Glycobiology}}, title = {{PAPST1 regulates sulfation of heparan sulfate proteoglycans in epithelial MDCK II cells.}}, url = {{http://dx.doi.org/10.1093/glycob/cwu084}}, doi = {{10.1093/glycob/cwu084}}, volume = {{25}}, year = {{2015}}, }