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Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression.

Malmström, Erik LU ; Davidova, A; Mörgelin, Matthias LU ; Linder, Adam LU ; Larsen, M; Qvortrup, K; Nordenfelt, Pontus LU ; Shannon, Oonagh LU ; Dzupova, O and Holub, M, et al. (2014) In Thrombosis and Haemostasis 112(6). p.1230-1243
Abstract
Early diagnosis of severe infectious diseases is essential for timely implementation of lifesaving therapies. In a search for novel biomarkers in sepsis diagnosis we focused on polymorphonuclear neutrophils (PMNs). Notably, PMNs have their protein cargo readily stored in granules and following systemic stimulation an immediate increase of neutrophil-borne proteins can be observed into the circulation of sepsis patients. We applied a combination of mass spectrometry (MS) based approaches, LC-MS/MS and selected reaction monitoring (SRM), to characterise and quantify the neutrophil proteome in healthy or disease conditions. With this approach we identified a neutrophil-derived protein abundance pattern in blood plasma consisting of 20... (More)
Early diagnosis of severe infectious diseases is essential for timely implementation of lifesaving therapies. In a search for novel biomarkers in sepsis diagnosis we focused on polymorphonuclear neutrophils (PMNs). Notably, PMNs have their protein cargo readily stored in granules and following systemic stimulation an immediate increase of neutrophil-borne proteins can be observed into the circulation of sepsis patients. We applied a combination of mass spectrometry (MS) based approaches, LC-MS/MS and selected reaction monitoring (SRM), to characterise and quantify the neutrophil proteome in healthy or disease conditions. With this approach we identified a neutrophil-derived protein abundance pattern in blood plasma consisting of 20 proteins that can be used as a protein signature for severe infectious diseases. Our results also show that SRM is highly sensitive, specific, and reproducible and, thus, a promising technology to study a complex, dynamic and multifactorial disease such as sepsis. (Less)
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Contribution to journal
publication status
published
subject
in
Thrombosis and Haemostasis
volume
112
issue
6
pages
1230 - 1243
publisher
F K Schattauer Verlag Gmbh
external identifiers
  • pmid:25104417
  • wos:000346040500020
  • scopus:84914169130
ISSN
0340-6245
DOI
10.1160/TH14-04-0312
language
English
LU publication?
yes
id
f50881d3-a385-4f7e-b593-2c2cab8b70ae (old id 4615368)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25104417?dopt=Abstract
date added to LUP
2014-09-04 18:15:34
date last changed
2017-07-02 04:06:01
@article{f50881d3-a385-4f7e-b593-2c2cab8b70ae,
  abstract     = {Early diagnosis of severe infectious diseases is essential for timely implementation of lifesaving therapies. In a search for novel biomarkers in sepsis diagnosis we focused on polymorphonuclear neutrophils (PMNs). Notably, PMNs have their protein cargo readily stored in granules and following systemic stimulation an immediate increase of neutrophil-borne proteins can be observed into the circulation of sepsis patients. We applied a combination of mass spectrometry (MS) based approaches, LC-MS/MS and selected reaction monitoring (SRM), to characterise and quantify the neutrophil proteome in healthy or disease conditions. With this approach we identified a neutrophil-derived protein abundance pattern in blood plasma consisting of 20 proteins that can be used as a protein signature for severe infectious diseases. Our results also show that SRM is highly sensitive, specific, and reproducible and, thus, a promising technology to study a complex, dynamic and multifactorial disease such as sepsis.},
  author       = {Malmström, Erik and Davidova, A and Mörgelin, Matthias and Linder, Adam and Larsen, M and Qvortrup, K and Nordenfelt, Pontus and Shannon, Oonagh and Dzupova, O and Holub, M and Malmström, Johan and Herwald, Heiko},
  issn         = {0340-6245},
  language     = {eng},
  number       = {6},
  pages        = {1230--1243},
  publisher    = {F K Schattauer Verlag Gmbh},
  series       = {Thrombosis and Haemostasis},
  title        = {Targeted mass spectrometry analysis of neutrophil-derived proteins released during sepsis progression.},
  url          = {http://dx.doi.org/10.1160/TH14-04-0312},
  volume       = {112},
  year         = {2014},
}