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Quantification of Osmotic Water Transport in vivo using Fluorescent Albumin.

Morelle, Johann; Sow, Amadou; Vertommen, Didier; Jamar, François; Rippe, Bengt LU and Devuyst, Olivier (2014) In American Journal of Physiology-Renal Physiology 307(8). p.981-989
Abstract
Osmotic water transport across the peritoneal membrane is applied during peritoneal dialysis to remove the excess of water accumulated in patients with end-stage renal disease. The discovery of aquaporin water channels and the generation of transgenic animals have stressed the need for novel and accurate methods to unravel molecular mechanisms of water permeability in vivo. Here we describe the use of fluorescently-labeled albumin as reliable indicator of osmotic water transport across the peritoneal membrane in a well-established mouse model of peritoneal dialysis. After detailed evaluation of the intraperitoneal tracer mass kinetics, the technique was validated against direct volumetry, considered as gold standard. The pH-insensitive dye... (More)
Osmotic water transport across the peritoneal membrane is applied during peritoneal dialysis to remove the excess of water accumulated in patients with end-stage renal disease. The discovery of aquaporin water channels and the generation of transgenic animals have stressed the need for novel and accurate methods to unravel molecular mechanisms of water permeability in vivo. Here we describe the use of fluorescently-labeled albumin as reliable indicator of osmotic water transport across the peritoneal membrane in a well-established mouse model of peritoneal dialysis. After detailed evaluation of the intraperitoneal tracer mass kinetics, the technique was validated against direct volumetry, considered as gold standard. The pH-insensitive dye AlexaFluor555-albumin was applied to quantify osmotic water transport across the mouse peritoneal membrane resulting from modulating dialysate osmolality and genetic silencing of the water channel AQP1. Quantification of osmotic water transport using AlexaFluor(555)-albumin closely correlated with direct volumetry and with estimations based on radio-iodinated ((125)I) serum albumin (RISA). The low intraperitoneal pressure probably accounts for the negligible disappearance of the tracer from the peritoneal cavity in this model. Taken together, these data demonstrate the appropriateness of the pH-insensitive AlexaFluor(555)-albumin as a practical and reliable intraperitoneal volume tracer to quantify osmotic water transport in vivo. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
American Journal of Physiology-Renal Physiology
volume
307
issue
8
pages
981 - 989
publisher
American Physiological Society
external identifiers
  • pmid:25100279
  • wos:000343233400012
  • scopus:84908078108
ISSN
1522-1466
DOI
10.1152/ajprenal.00098.2014
language
English
LU publication?
yes
id
fcdef47a-9b9c-4464-ae17-404413fa66f1 (old id 4615466)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25100279?dopt=Abstract
date added to LUP
2014-09-04 17:38:45
date last changed
2017-09-10 03:19:27
@article{fcdef47a-9b9c-4464-ae17-404413fa66f1,
  abstract     = {Osmotic water transport across the peritoneal membrane is applied during peritoneal dialysis to remove the excess of water accumulated in patients with end-stage renal disease. The discovery of aquaporin water channels and the generation of transgenic animals have stressed the need for novel and accurate methods to unravel molecular mechanisms of water permeability in vivo. Here we describe the use of fluorescently-labeled albumin as reliable indicator of osmotic water transport across the peritoneal membrane in a well-established mouse model of peritoneal dialysis. After detailed evaluation of the intraperitoneal tracer mass kinetics, the technique was validated against direct volumetry, considered as gold standard. The pH-insensitive dye AlexaFluor555-albumin was applied to quantify osmotic water transport across the mouse peritoneal membrane resulting from modulating dialysate osmolality and genetic silencing of the water channel AQP1. Quantification of osmotic water transport using AlexaFluor(555)-albumin closely correlated with direct volumetry and with estimations based on radio-iodinated ((125)I) serum albumin (RISA). The low intraperitoneal pressure probably accounts for the negligible disappearance of the tracer from the peritoneal cavity in this model. Taken together, these data demonstrate the appropriateness of the pH-insensitive AlexaFluor(555)-albumin as a practical and reliable intraperitoneal volume tracer to quantify osmotic water transport in vivo.},
  author       = {Morelle, Johann and Sow, Amadou and Vertommen, Didier and Jamar, François and Rippe, Bengt and Devuyst, Olivier},
  issn         = {1522-1466},
  language     = {eng},
  number       = {8},
  pages        = {981--989},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology-Renal Physiology},
  title        = {Quantification of Osmotic Water Transport in vivo using Fluorescent Albumin.},
  url          = {http://dx.doi.org/10.1152/ajprenal.00098.2014},
  volume       = {307},
  year         = {2014},
}