Quantification of Osmotic Water Transport in vivo using Fluorescent Albumin.
(2014) In American Journal of Physiology-Renal Physiology 307(8). p.981-989- Abstract
- Osmotic water transport across the peritoneal membrane is applied during peritoneal dialysis to remove the excess of water accumulated in patients with end-stage renal disease. The discovery of aquaporin water channels and the generation of transgenic animals have stressed the need for novel and accurate methods to unravel molecular mechanisms of water permeability in vivo. Here we describe the use of fluorescently-labeled albumin as reliable indicator of osmotic water transport across the peritoneal membrane in a well-established mouse model of peritoneal dialysis. After detailed evaluation of the intraperitoneal tracer mass kinetics, the technique was validated against direct volumetry, considered as gold standard. The pH-insensitive dye... (More)
- Osmotic water transport across the peritoneal membrane is applied during peritoneal dialysis to remove the excess of water accumulated in patients with end-stage renal disease. The discovery of aquaporin water channels and the generation of transgenic animals have stressed the need for novel and accurate methods to unravel molecular mechanisms of water permeability in vivo. Here we describe the use of fluorescently-labeled albumin as reliable indicator of osmotic water transport across the peritoneal membrane in a well-established mouse model of peritoneal dialysis. After detailed evaluation of the intraperitoneal tracer mass kinetics, the technique was validated against direct volumetry, considered as gold standard. The pH-insensitive dye AlexaFluor555-albumin was applied to quantify osmotic water transport across the mouse peritoneal membrane resulting from modulating dialysate osmolality and genetic silencing of the water channel AQP1. Quantification of osmotic water transport using AlexaFluor(555)-albumin closely correlated with direct volumetry and with estimations based on radio-iodinated ((125)I) serum albumin (RISA). The low intraperitoneal pressure probably accounts for the negligible disappearance of the tracer from the peritoneal cavity in this model. Taken together, these data demonstrate the appropriateness of the pH-insensitive AlexaFluor(555)-albumin as a practical and reliable intraperitoneal volume tracer to quantify osmotic water transport in vivo. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4615466
- author
- Morelle, Johann ; Sow, Amadou ; Vertommen, Didier ; Jamar, François ; Rippe, Bengt LU and Devuyst, Olivier
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Physiology-Renal Physiology
- volume
- 307
- issue
- 8
- pages
- 981 - 989
- publisher
- American Physiological Society
- external identifiers
-
- pmid:25100279
- wos:000343233400012
- scopus:84908078108
- pmid:25100279
- ISSN
- 1522-1466
- DOI
- 10.1152/ajprenal.00098.2014
- language
- English
- LU publication?
- yes
- id
- fcdef47a-9b9c-4464-ae17-404413fa66f1 (old id 4615466)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25100279?dopt=Abstract
- date added to LUP
- 2016-04-01 10:42:30
- date last changed
- 2022-01-26 01:47:06
@article{fcdef47a-9b9c-4464-ae17-404413fa66f1,
abstract = {{Osmotic water transport across the peritoneal membrane is applied during peritoneal dialysis to remove the excess of water accumulated in patients with end-stage renal disease. The discovery of aquaporin water channels and the generation of transgenic animals have stressed the need for novel and accurate methods to unravel molecular mechanisms of water permeability in vivo. Here we describe the use of fluorescently-labeled albumin as reliable indicator of osmotic water transport across the peritoneal membrane in a well-established mouse model of peritoneal dialysis. After detailed evaluation of the intraperitoneal tracer mass kinetics, the technique was validated against direct volumetry, considered as gold standard. The pH-insensitive dye AlexaFluor555-albumin was applied to quantify osmotic water transport across the mouse peritoneal membrane resulting from modulating dialysate osmolality and genetic silencing of the water channel AQP1. Quantification of osmotic water transport using AlexaFluor(555)-albumin closely correlated with direct volumetry and with estimations based on radio-iodinated ((125)I) serum albumin (RISA). The low intraperitoneal pressure probably accounts for the negligible disappearance of the tracer from the peritoneal cavity in this model. Taken together, these data demonstrate the appropriateness of the pH-insensitive AlexaFluor(555)-albumin as a practical and reliable intraperitoneal volume tracer to quantify osmotic water transport in vivo.}},
author = {{Morelle, Johann and Sow, Amadou and Vertommen, Didier and Jamar, François and Rippe, Bengt and Devuyst, Olivier}},
issn = {{1522-1466}},
language = {{eng}},
number = {{8}},
pages = {{981--989}},
publisher = {{American Physiological Society}},
series = {{American Journal of Physiology-Renal Physiology}},
title = {{Quantification of Osmotic Water Transport in vivo using Fluorescent Albumin.}},
url = {{http://dx.doi.org/10.1152/ajprenal.00098.2014}},
doi = {{10.1152/ajprenal.00098.2014}},
volume = {{307}},
year = {{2014}},
}