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Cross-species utility of 22 microsatellite markers in the Melodious Warbler (Hippolais polyglotta)

Engler, Jan O.; Secondi, Jean LU ; Dawson, Deborah A.; Roderus, David; Elle, Ortwin and Hochkirch, Axel (2014) In Avian Biology Research 7(2). p.91-98
Abstract
Microsatellites are a valuable tool in the analysis of population genetic structure. Utilising microsatellite markers that were originally isolated from other species (cross-species amplification) can prove an efficient way, in terms of time and cost, to obtain markers for genetic studies. Here, 55 avian microsatellite primer pairs were tested for the cross-amplification in the Melodious Warbler (Hippolaispo/yglotta). Thirty-five markers amplified, of which 22 were polymorphic, displaying two to nine alleles in the 15 individuals genotyped. The 35 markers which amplified in the Melodious Warbler were tested in its sister species the lcterine Warbler (H. icterina). Twenty-four markers were amplified, 14 of which were polymorphic in the five... (More)
Microsatellites are a valuable tool in the analysis of population genetic structure. Utilising microsatellite markers that were originally isolated from other species (cross-species amplification) can prove an efficient way, in terms of time and cost, to obtain markers for genetic studies. Here, 55 avian microsatellite primer pairs were tested for the cross-amplification in the Melodious Warbler (Hippolaispo/yglotta). Thirty-five markers amplified, of which 22 were polymorphic, displaying two to nine alleles in the 15 individuals genotyped. The 35 markers which amplified in the Melodious Warbler were tested in its sister species the lcterine Warbler (H. icterina). Twenty-four markers were amplified, 14 of which were polymorphic in the five H. icterina individuals genotyped. Thirteen loci were polymorphic in both species. The polymorphic loci identified are suitable for analysing the genetic population structure and assigning parentage. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cross-species amplification, Hippolais, microsatellite, simple tandem, repeat
in
Avian Biology Research
volume
7
issue
2
pages
91 - 98
publisher
Science Reviews 2000 Ltd
external identifiers
  • wos:000340217100004
  • scopus:84902257478
ISSN
1758-1567
DOI
10.3184/175815514X13947968713023
language
English
LU publication?
yes
id
399c8aeb-e58d-49b8-9c66-69db809748f6 (old id 4665587)
date added to LUP
2014-09-25 14:45:46
date last changed
2017-08-20 03:25:49
@article{399c8aeb-e58d-49b8-9c66-69db809748f6,
  abstract     = {Microsatellites are a valuable tool in the analysis of population genetic structure. Utilising microsatellite markers that were originally isolated from other species (cross-species amplification) can prove an efficient way, in terms of time and cost, to obtain markers for genetic studies. Here, 55 avian microsatellite primer pairs were tested for the cross-amplification in the Melodious Warbler (Hippolaispo/yglotta). Thirty-five markers amplified, of which 22 were polymorphic, displaying two to nine alleles in the 15 individuals genotyped. The 35 markers which amplified in the Melodious Warbler were tested in its sister species the lcterine Warbler (H. icterina). Twenty-four markers were amplified, 14 of which were polymorphic in the five H. icterina individuals genotyped. Thirteen loci were polymorphic in both species. The polymorphic loci identified are suitable for analysing the genetic population structure and assigning parentage.},
  author       = {Engler, Jan O. and Secondi, Jean and Dawson, Deborah A. and Roderus, David and Elle, Ortwin and Hochkirch, Axel},
  issn         = {1758-1567},
  keyword      = {cross-species amplification,Hippolais,microsatellite,simple tandem,repeat},
  language     = {eng},
  number       = {2},
  pages        = {91--98},
  publisher    = {Science Reviews 2000 Ltd},
  series       = {Avian Biology Research},
  title        = {Cross-species utility of 22 microsatellite markers in the Melodious Warbler (Hippolais polyglotta)},
  url          = {http://dx.doi.org/10.3184/175815514X13947968713023},
  volume       = {7},
  year         = {2014},
}