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Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and in type 2 diabetic Goto-Kakizaki rats.

Stenberg, Lena LU and Dahlin, Lars LU (2014) In BMC Neuroscience 15(1).
Abstract
In view of the global increase in diabetes, and the fact that recent findings indicate that diabetic neuropathy is more frequently seen in males, it is crucial to evaluate any gender differences in nerve regeneration in diabetes. Our aim was to evaluate in short-term experiments gender dissimilarities in axonal outgrowth in healthy and in genetically developed type 2 diabetic Goto-Kakizaki (GK) rats, and also to investigate the connection between activated (i.e. ATF-3, Activating Transcription Factor 3) and apoptotic (cleaved caspase 3) Schwann cells after sciatic nerve injury and repair. Female and male diabetic GK rats, spontaneously developing type 2 diabetes, were compared with corresponding healthy Wistar rats. The sciatic nerve was... (More)
In view of the global increase in diabetes, and the fact that recent findings indicate that diabetic neuropathy is more frequently seen in males, it is crucial to evaluate any gender differences in nerve regeneration in diabetes. Our aim was to evaluate in short-term experiments gender dissimilarities in axonal outgrowth in healthy and in genetically developed type 2 diabetic Goto-Kakizaki (GK) rats, and also to investigate the connection between activated (i.e. ATF-3, Activating Transcription Factor 3) and apoptotic (cleaved caspase 3) Schwann cells after sciatic nerve injury and repair. Female and male diabetic GK rats, spontaneously developing type 2 diabetes, were compared with corresponding healthy Wistar rats. The sciatic nerve was transected and instantly repaired. After six days the nerve was harvested to measure axonal outgrowth (i.e. neurofilament staining), and to quantify the number of ATF-3 (i.e. activated) and cleaved caspase 3 (i.e. apoptotic) stained Schwann cells using immunohistochemistry. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
BMC Neuroscience
volume
15
issue
1
publisher
BioMed Central
external identifiers
  • pmid:25216784
  • wos:000342366700001
  • scopus:84907280999
ISSN
1471-2202
DOI
10.1186/1471-2202-15-107
language
English
LU publication?
yes
id
84d6a244-512b-488c-827f-535cbfb3778e (old id 4691669)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25216784?dopt=Abstract
date added to LUP
2014-10-06 20:42:37
date last changed
2017-11-19 03:53:01
@article{84d6a244-512b-488c-827f-535cbfb3778e,
  abstract     = {In view of the global increase in diabetes, and the fact that recent findings indicate that diabetic neuropathy is more frequently seen in males, it is crucial to evaluate any gender differences in nerve regeneration in diabetes. Our aim was to evaluate in short-term experiments gender dissimilarities in axonal outgrowth in healthy and in genetically developed type 2 diabetic Goto-Kakizaki (GK) rats, and also to investigate the connection between activated (i.e. ATF-3, Activating Transcription Factor 3) and apoptotic (cleaved caspase 3) Schwann cells after sciatic nerve injury and repair. Female and male diabetic GK rats, spontaneously developing type 2 diabetes, were compared with corresponding healthy Wistar rats. The sciatic nerve was transected and instantly repaired. After six days the nerve was harvested to measure axonal outgrowth (i.e. neurofilament staining), and to quantify the number of ATF-3 (i.e. activated) and cleaved caspase 3 (i.e. apoptotic) stained Schwann cells using immunohistochemistry.},
  articleno    = {107},
  author       = {Stenberg, Lena and Dahlin, Lars},
  issn         = {1471-2202},
  language     = {eng},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {BMC Neuroscience},
  title        = {Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and in type 2 diabetic Goto-Kakizaki rats.},
  url          = {http://dx.doi.org/10.1186/1471-2202-15-107},
  volume       = {15},
  year         = {2014},
}